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Array sensor for detecting pesticide residues, and preparation method and application of array sensor

An array sensor and pesticide residue technology, applied in the field of analytical chemistry, can solve problems such as powerlessness and no strong interaction, and achieve the effects of simple production process, wide range of detection objects and low cost

Active Publication Date: 2019-06-18
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Then there will be such a problem that the visual array sensing technology is incapable of detecting those analytes that do not have a strong interaction with the sensing unit, no chromophore, etc. (ie weakly reactive analytes, such as pesticides)

Method used

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  • Array sensor for detecting pesticide residues, and preparation method and application of array sensor
  • Array sensor for detecting pesticide residues, and preparation method and application of array sensor
  • Array sensor for detecting pesticide residues, and preparation method and application of array sensor

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preparation example Construction

[0027] As an aspect of the technical solution of the present invention, it relates to a method for preparing an array sensor for detecting pesticide residues, which includes:

[0028] Provide a series of uniform mixed systems containing different concentrations of potassium permanganate and / or potassium dichromate and sulfuric acid as the detection solution;

[0029] Each detection solution is respectively placed in each sensing unit of the array sensor to prepare an array sensor for detecting pesticide residues.

[0030] In one embodiment, the concentration of potassium permanganate or potassium dichromate in the detection solution ranges from 0.01 to 0.1 mMol / L.

[0031] Further, the concentration of sulfuric acid in the detection solution ranges from 0.1 to 1.0 Mol / L.

[0032] Further, the molar ratio of potassium permanganate and / or potassium dichromate to sulfuric acid in the detection solution ranges from 0.00001 to 0.001:1.

[0033] Further, there are 2 to 10 sensing ...

Embodiment 1

[0050] Embodiment 1 realizes the identification and distinction to multiple pesticide residues

[0051] Standard solutions of the same concentration and different pesticide residues are respectively added to each sensing unit of the array sensor and mixed with the detection solution therein. The sensing units of the array sensor for detecting pesticide residues are mixed with different pesticide residues Different color changes were produced after the reaction, and the color changes of each mixed reaction system were read by a scanner; the color changes read by the scanner were digitally processed by Photoshop software to obtain the RGB values ​​corresponding to the front and rear images of different sensing units, and the The RGB value of the image after the reaction is subtracted from the RGB value of the image before the reaction, and the △R, △G and △B values ​​of the subtraction image are extracted, and the experiment is paralleled 5 times; then a series of △R, △G and △B va...

Embodiment 2

[0052] The establishment of embodiment 2 pesticide residue standard fitting curve

[0053] A series of standard solutions containing different concentrations of pesticide residues were added to each sensing unit of the array sensor and mixed with the detection solution therein, and a certain volume was put into a 96-well plate, which was used to detect pesticide residues The sensing unit of the array sensor reacts with the pesticide residues to produce different color changes, and the color changes of each mixed reaction system are read by a scanner; the color changes read by the scanner are digitally processed by Photoshop software, and different color changes are obtained. The RGB values ​​corresponding to the front and rear images of the sensing unit are subtracted from the RGB values ​​of the post-reaction image by the RGB values ​​of the pre-reaction image to extract the △R, △G and △B values ​​of the subtracted image, and the △R, △G and △B The value is converted into the ...

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Abstract

The invention discloses an array sensor for detecting pesticide residues, and a preparation method and an application of the array sensor. The preparation method comprises the steps of providing a series of uniformly mixed systems containing potassium permanganate and / or potassium dichromate and sulfuric acid with different concentrations as detection liquids; and putting the detection liquids into sensing units of the array sensor to prepare the array sensor for detecting the pesticide residues. The array sensor for detecting the pesticide residues can realize qualitative and semi-quantitative detection of various pesticide residues; meanwhile, the array sensor is simple in preparation process, high in detection speed and relatively high in detection sensitivity; and a basis and a possibility are provided for realizing the application of an array sensing technology to the detection of a weak reactive analyte.

Description

technical field [0001] The present invention relates to an array sensor for detecting pesticide residues, in particular to an array sensor for detecting pesticide residues and a preparation method thereof, and the application of the array sensor in detecting pesticide residues, belonging to analytical chemistry technology field. Background technique [0002] Pesticide residues (referred to as pesticide residues) commonly used analysis and detection methods mainly include gas chromatography (GC), high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (HPLC-MS) Wait. Although these methods have the advantages of high sensitivity and accurate quantification, the detection steps are cumbersome, the detection cycle is relatively long, and expensive large-scale instruments and professional technicians are required, so real-time detection cannot be achieved on site. Sensor technology has gradually becom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 钱四化林恒伟王宇辉乔丽娜
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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