Method for detecting target object by spectrophotometry method or fluorescence method
A technology for spectrophotometry and target detection, which is applied in the field of target detection by spectrophotometry or fluorescence, which can solve the problems of cumbersome operation steps, cumbersome detection of light, and high operation requirements, and achieve a wide range of detection values, easy operation, and high accuracy. high effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0097] Embodiment 1 Spectrophotometry detects ions or small biomolecules
[0098] A. Determination of calcium content in milk
[0099] Raw milk samples were purchased from the Analysis and Measurement Center of Mengniu Group.
[0100] The concentration of the calcium calibrator is 100mg / 100mL.
[0101] Spectrophotometric detection using a multifunction device:
[0102] (1) Sample dilution: Take 100 μl of raw milk sample and 900 μl of diluent and add them into EP tubes, shake and mix to obtain sample milk. Wherein, the diluent 1 is the following components: 0.5% sodium hydroxide, and the percentages are mass percentages. Diluent 2 has the following components: 2% hydrochloric acid and 0.5% Triton X-100, and the percentages are mass percentages.
[0103] (2) Shake and mix the sample milk, water and calcium calibration product obtained in step (1) respectively with the determination reagent, and then react in a water bath at 37°C for 5 minutes to obtain a sample solution, a bla...
Embodiment 2
[0117] Example 2 Detection of small biological molecules using small molecule chromatography test paper fluorescence method
[0118] Determination of Melamine Content in Milk
[0119] Take 100 μl of raw milk sample, add it into the freeze-dried microwells with fluorescent probes, and then insert chromatography test strips, wherein, the preparation method of the fluorescent probes and the chromatography test papers can be found in Chinese patent application CN103013492A, published on 2013 April 13th.
[0120] One end of the sample pad of the chromatography test paper is immersed in the liquid, inserted into the test strip carrier 17 of the multifunctional device of the present invention after immersion for 5 minutes, the test strip carrier 17 is inserted into the second opening 4, and detection is started, and the detection result shows On the LED screen 31.
[0121] It was found that the lower limit of quantitative detection of melamine in raw milk samples was 10 ng / mL, and ...
Embodiment 3
[0122] Example 3 Detection of biological macromolecules using macromolecular chromatography test paper fluorescence method
[0123] Determination of the content of human pregnancy-associated protein A (PAPP-A) and free human chorionic gonadotropin β subunit (Free-β-HCG) in samples such as serum, plasma and whole blood
[0124] Take 200 μl of diluent and add it to the sample tube, add 20 μl of serum, plasma and whole blood, and mix well. Take 200 μl of the clarified sample solution after sample pretreatment and add it into the sample hole of the fluorescence quantitative detection test strip. Wherein, the preparation method of the fluorescent quantitative detection test strip refers to Chinese patent application CN 103760369A, published on April 30, 2014. After reacting for 5-8 minutes, insert the test strip carrier 17 of the multifunctional device of the present invention, insert the test strip carrier 17 into the second opening 4, start detection, and the detection result is...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com



