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Method for detecting target object by spectrophotometry method or fluorescence method

A technology for spectrophotometry and target detection, which is applied in the field of target detection by spectrophotometry or fluorescence, which can solve the problems of cumbersome operation steps, cumbersome detection of light, and high operation requirements, and achieve a wide range of detection values, easy operation, and high accuracy. high effect

Inactive Publication Date: 2018-02-06
上海溯源生物技术有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the spectrophotometric detection method and the fluorescence detection method need to use different detection devices, and the operation steps of these detection devices are often very cumbersome, it takes a lot of time, and sometimes even requires the use of large instruments or dangerous gases such as acetylene. relatively high
[0003] In addition, current devices cannot use both spectrophotometry and fluorescence methods, and researchers need to replace the detection device from time to time in daily detection according to the detection object
At the same time, because different detection objects are suitable for different test strips used in fluorescence methods, and are suitable for the detection light corresponding to different test strips, conventional devices in this field require manual repeated inspection and adjustment of detection light is very cumbersome

Method used

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  • Method for detecting target object by spectrophotometry method or fluorescence method
  • Method for detecting target object by spectrophotometry method or fluorescence method
  • Method for detecting target object by spectrophotometry method or fluorescence method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Embodiment 1 Spectrophotometry detects ions or small biomolecules

[0098] A. Determination of calcium content in milk

[0099] Raw milk samples were purchased from the Analysis and Measurement Center of Mengniu Group.

[0100] The concentration of the calcium calibrator is 100mg / 100mL.

[0101] Spectrophotometric detection using a multifunction device:

[0102] (1) Sample dilution: Take 100 μl of raw milk sample and 900 μl of diluent and add them into EP tubes, shake and mix to obtain sample milk. Wherein, the diluent 1 is the following components: 0.5% sodium hydroxide, and the percentages are mass percentages. Diluent 2 has the following components: 2% hydrochloric acid and 0.5% Triton X-100, and the percentages are mass percentages.

[0103] (2) Shake and mix the sample milk, water and calcium calibration product obtained in step (1) respectively with the determination reagent, and then react in a water bath at 37°C for 5 minutes to obtain a sample solution, a bla...

Embodiment 2

[0117] Example 2 Detection of small biological molecules using small molecule chromatography test paper fluorescence method

[0118] Determination of Melamine Content in Milk

[0119] Take 100 μl of raw milk sample, add it into the freeze-dried microwells with fluorescent probes, and then insert chromatography test strips, wherein, the preparation method of the fluorescent probes and the chromatography test papers can be found in Chinese patent application CN103013492A, published on 2013 April 13th.

[0120] One end of the sample pad of the chromatography test paper is immersed in the liquid, inserted into the test strip carrier 17 of the multifunctional device of the present invention after immersion for 5 minutes, the test strip carrier 17 is inserted into the second opening 4, and detection is started, and the detection result shows On the LED screen 31.

[0121] It was found that the lower limit of quantitative detection of melamine in raw milk samples was 10 ng / mL, and ...

Embodiment 3

[0122] Example 3 Detection of biological macromolecules using macromolecular chromatography test paper fluorescence method

[0123] Determination of the content of human pregnancy-associated protein A (PAPP-A) and free human chorionic gonadotropin β subunit (Free-β-HCG) in samples such as serum, plasma and whole blood

[0124] Take 200 μl of diluent and add it to the sample tube, add 20 μl of serum, plasma and whole blood, and mix well. Take 200 μl of the clarified sample solution after sample pretreatment and add it into the sample hole of the fluorescence quantitative detection test strip. Wherein, the preparation method of the fluorescent quantitative detection test strip refers to Chinese patent application CN 103760369A, published on April 30, 2014. After reacting for 5-8 minutes, insert the test strip carrier 17 of the multifunctional device of the present invention, insert the test strip carrier 17 into the second opening 4, start detection, and the detection result is...

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Abstract

The invention discloses a method for detecting a target object by a spectrophotometry method or a fluorescence method. The method comprises the following step of putting a sample to be detected into adetection device to be detected, wherein the detecting device is a multifunctional detection device comprising a detection platform, a first light source, a second light source and a receiving device; the detection platform carries samples to be detected; the first light source is positioned above the detection platform and emits first light to the detection platform; the second light source is positioned under the detection platform and emits second light to the detection platform; the receiving device is positioned above the detection platform and receives detection light from the sample tobe detected. The method has the advantages that both the spectrophotometry method and the fluorescence method are used; the operation is simple and convenient; the accuracy is high; the detection object range is wide; the detection value range is wide.

Description

technical field [0001] This field relates to the field of biomedicine, in particular to a method for detecting target objects by spectrophotometry or fluorescence. Background technique [0002] For researchers in the field of biomedicine, the current conventional methods for detecting calcium, iron, zinc, sodium, potassium, magnesium, copper and manganese ions or small molecules such as melamine are spectrophotometric or fluorescent detection methods. However, the spectrophotometric detection method and the fluorescence detection method need to use different detection devices, and the operation steps of these detection devices are often very cumbersome, it takes a lot of time, and sometimes even requires the use of large instruments or dangerous gases such as acetylene. relatively high. [0003] In addition, current devices cannot use both spectrophotometry and fluorescence methods. In daily detection, researchers need to replace detection devices from time to time accordin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N21/64G01N33/76G01N33/72G01N33/558
CPCG01N21/33G01N21/6408G01N21/6486G01N33/558G01N33/72G01N33/76G01N2201/061G01N2201/0618
Inventor 刘斌虎余欧明梁俊张琪
Owner 上海溯源生物技术有限公司
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