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Method for separation and detection of mycotoxins produced by corn during transportation in bulk grain containers

A transportation process, mycotoxin technology, applied in the field of detection, can solve the problems of endangering the health of livestock and poultry, endangering human health, economic losses of livestock and poultry enterprises, etc.

Active Publication Date: 2022-03-01
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Mycotoxins seriously endanger the health of livestock and poultry in production, indirectly endanger human health, and cause serious economic losses to livestock and poultry enterprises

Method used

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  • Method for separation and detection of mycotoxins produced by corn during transportation in bulk grain containers
  • Method for separation and detection of mycotoxins produced by corn during transportation in bulk grain containers
  • Method for separation and detection of mycotoxins produced by corn during transportation in bulk grain containers

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Embodiment Construction

[0037] The method for separating and detecting mycotoxins produced by corn during bulk grain container transportation provided by the present invention can not only quickly and thoroughly separate mycotoxins from high-fat, high-protein complex matrices, but also quickly and effectively detect Mycotoxins in corn, the research on the separation and detection method is a project of the National Key Research and Development Program (2018YFD0401403).

[0038] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0039] 1. Prepare samples

[0040] Three varieties of corn samples in the container were randomly selected.

[0041] The three varieties of corn samples were pulverized separately to ensure that at least 75% of the corn flour of each variety of corn samples passed through the 80-mesh sieve, and the corn flour of each variety of corn samples passed the 80-mesh sieve and did not pass through the ...

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Abstract

The invention discloses a method for separating and detecting mycotoxins produced by corn during transportation in bulk grain containers. Compared, the extraction agent of the present invention is cheaper, more environmentally friendly and the obtained extract is cleaner; the mass mixed reagents such as diatomaceous earth and ethylenediamine-N-propylsilane bonded phase silica gel are used as the adsorbent, the adsorbent The analyte is adsorbed in a non-polar system, the amount of fat removal is large, and the fat content in the extract has little effect on the extraction efficiency. It can be seen that this separation method expands the selection of mycotoxin extractants in high-fat solid samples. And the optimization of mixed adsorbents is promising in improving the extraction and purification efficiency of mycotoxins in high-fat solid samples.

Description

technical field [0001] The invention relates to a method for separating and detecting mycotoxins, in particular to a method for separating and detecting mycotoxins produced by corn during bulk grain container transportation, and belongs to the technical field of detection. Background technique [0002] In 1960, about 100,000 turkeys died in the UK due to acute poisoning by aflatoxin. In the following year, it was confirmed that aflatoxin has serious carcinogenicity. Since then, mycotoxin has attracted widespread attention. [0003] Mycotoxins, also known as mycotoxins, are a series of toxic secondary metabolites produced by some molds (mainly including Aspergillus, Penicillium and Fusarium) after they mature. According to the different growth environments of molds, mycotoxins can be divided into two types: field toxins and storage toxins. The identification and classification of molds are traditionally based on morphological and cultural characteristics, but the morphologic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34
Inventor 张丽媛钱丽丽于英博张东杰
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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