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Method for establishing HPLC (high-performance liquid chromatography) fingerprint spectrum of Shuganning injection

A technology for fingerprinting and establishing methods, which can be applied to instruments, measuring devices, scientific instruments, etc., and can solve the problems of limiting the technical improvement and in-depth development of Shuganning injection.

Active Publication Date: 2019-06-25
GUIZHOU RUIHE PHARMA CO LTD LONGLI PHARMA FACTORY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the fingerprint conditions of Shuganning Injection fail to fully reflect the characteristic components of Ganoderma lucidum extract in the prescription, which limits the technical improvement and in-depth development of Shuganning Injection

Method used

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  • Method for establishing HPLC (high-performance liquid chromatography) fingerprint spectrum of Shuganning injection
  • Method for establishing HPLC (high-performance liquid chromatography) fingerprint spectrum of Shuganning injection
  • Method for establishing HPLC (high-performance liquid chromatography) fingerprint spectrum of Shuganning injection

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Embodiment 1

[0233] A method for establishing the HPLC fingerprint of Shuganning Injection, characterized in that: the Shuganning Injection is mainly extracted from capillary wormwood extract 4g, Gardenia jasmine extract 3g, baicalin 22g, isatis root extract 5g and ganoderma lucidum 3.5g of the product is prepared according to the following method: for the above five flavors, take baicalin and add 10 times the amount of water for injection to suspend, and add 1200ml of 10% sodium hydroxide solution to dissolve; Dissolve in water, mix well, add 0.2% activated carbon, stir well, boil for 15 minutes, filter, adjust the pH value to 7.5-8.0 with 10% sodium hydroxide solution, add water for injection to 1000mL, filter, potting, and sterilize , that is;

[0234] The establishment method of described HPLC fingerprint collection, comprises the following steps:

[0235] (1) Preparation of reference solution: take adenosine reference substance, thymidine reference substance, ganoderma acid A referen...

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Abstract

The invention discloses a method for establishing the HPLC (high-performance liquid chromatography) fingerprint spectrum of Shuganning injection. According to the method of the invention, on the basisof high polarity of the components of a ganoderma lucidum extract, the injection finished product fingerprint spectrum of the Ganoderma lucidum extract is established; mobile phases, detection wavelengths and other high-performance liquid chromatography conditions are optimized, and comprehensive methodological investigation is carried out; it can be seen from systematic research that the peaks of the characteristic components of a Ganoderma lucidum medicinal material, the Ganoderma lucidum extract and a finished preparation have high correlation; the unique chemical components of the Ganoderma lucidum can be transferred to the finished preparation; and the fingerprint spectrum can better reflect the unique components of the Ganoderma lucidum. With the method adopted, the types and quantities of chemical components contained in the Shuganning injection can be comprehensively reflected, and then, overall description and evaluation can be performed on the quality of the Shuganning injection; many chemical components are contained in the fingerprint spectrum of the Shuganning injection detected by the method; the height ratios of the characteristic peaks of the chemical components are moderate; the baseline of the fingerprint spectrum is stable; and the resolution, peak shape and column efficiency of the fingerprint spectrum are good.

Description

technical field [0001] The invention relates to a method for establishing HPLC fingerprints of medicines, in particular to a method for establishing HPLC fingerprints of Shuganning injection. technical background [0002] Shuganning injection is a liver-protecting drug, mainly made of capillary extract, gardenia extract, baicalin, isatis root extract and ganoderma lucidum extract. It has the effects of clearing away heat and detoxification, promoting dampness and reducing jaundice, nourishing qi and strengthening the body, and protecting the liver. It is used for damp-heat jaundice, yellow eyes and eyes, fullness of breast ribs, nausea and vomiting, yellow and red urine, fatigue, anorexia, and loose stools; acute and chronic viral hepatitis see the aforementioned symptoms. [0003] Shuganning Injection was developed from an ancient prescription of traditional Chinese medicine. Limited by the level of scientific research at that time, its quality standard was relatively low,...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 赵云张昀何峰原勤熊毅刚
Owner GUIZHOU RUIHE PHARMA CO LTD LONGLI PHARMA FACTORY
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