A kind of chrysanthemum chrysanthemum leafhopper repellant and its application method
A technology of small green leafhoppers and repellents, applied in animal repellants, botanical equipment and methods, applications, etc., can solve problems such as excessive chemical pesticide residues
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Embodiment 1
[0019] The effect of embodiment 1 lavender plant odor repelling chrysanthemum small green leafhopper
[0020] 1 Experimental materials
[0021] Insects tested: Chrysanthemum chrysanthemum leafhoppers were collected from the Chrysanthemum chrysanthemum nursery of Tongxiang Agricultural Bureau, Zhejiang Province, and inoculated in the chrysanthemum garden of China Jiliang University to reproduce on the chrysanthemum plants generation by generation. Adults were collected from the chrysanthemum plants before the experiment and fasted for 0.5 hours.
[0022] Flavor source of lavender for testing: Lavender was planted by the chrysanthemum garden of China Jiliang University, and its top branches and leaves were cut as plant flavor sources. There were 7 flavors of 1.0g, 2.0g, 3.0g, 4.0g, 5.00g, 6.00g and 7.00g. dose. Take clean air as CK.
[0023] 2 Y-shape olfactometer and the behavior determination procedure of Chrysanthemum chrysanthemum leafhopper
[0024] The Y-shaped tube is...
Embodiment 2
[0030] The identification of embodiment 2 lavender plant odor
[0031] 1. Collection of lavender plant odor
[0032] Put the lavender tender stems into a glass cylinder (diameter 20cm, volume 4L). The cylinder consists of two parts that can be ground in tightly, or opened and tightened after the sample is placed. There is an air inlet and an air outlet at both ends of the cylinder. The air inlet is connected to an air filter (filled with activated carbon) and a flow meter in turn, and the air outlet is connected to a Super Q adsorption column (100mg), a water absorption device, and an air pump in turn. The parts are connected with Teflon tubes. The flow rate is 500mL / min, continuous pumping, the flow rate of the gas outlet is slightly smaller than the flow rate of the inlet port, so as to ensure that the cylinder is filled with clean air. Vials accept. Add 10 μL of 10 -4 g / mL ethyl caprate was used as the internal standard, and after shaking well, draw 1 μL into the GC-MS...
Embodiment 3
[0044] Example 3 The repellency of main components of indoor lavender odor for Chrysanthemum chrysanthemum leafhopper
[0045] The main components with a relative content greater than 500 in Table 1 are α-phellandrene, E-ocimene, 2-isopropyl-4-methylanisole, thymol, β-parsnolene, terpinolene and lauryl Moderate olefin content. These 7 compounds were formulated into 10 with n-hexane -2 g / mL solution as a taste source. Draw 1mL into the open ampoule bottle, put the ampoule bottle into the empty flavor source bottle of the above-mentioned embodiment 1; then inject 1mL of n-hexane into the open ampoule bottle, and put it into the empty control bottle of the above-mentioned embodiment 1 As a control, according to the procedure of Example 1, the behavioral measurement was carried out on Leafhopper chrysanthemum (Table 2).
[0046] Results: Thymol and 2-isopropyl-4-methylanisole repelled Chrysanthemum chrysanthemum very significantly, terpinolene and myrcene repelled significantly...
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