A kind of phthalate compound and its extraction method
An extraction method and compound technology, applied in the fields of organic chemistry, antibacterial drugs, and resistance to vector-borne diseases, etc., can solve the problems of drug resistance, pain, and increased antibacterial effects of bacteria, and achieve the effect of not easy to relapse and good antibacterial effect
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Embodiment 1
[0028] A method for extracting phthalein compounds, comprising the following steps:
[0029] (1) Cultivate Pseudomonas genus in rice culture medium, then soak with methanol for 24 hours, filter, and get the filtrate to obtain a crude extract for subsequent use;
[0030] (2) Extract the crude extract prepared in step (1) with ethyl acetate, collect the ethyl acetate phase, concentrate and dry under reduced pressure with a rotary evaporator to obtain the extract;
[0031] (3) The extract prepared in step (2) is separated by column chromatography, then separated by high performance liquid chromatography, concentrated by a rotary evaporator under reduced pressure, and dried in a vacuum drying oven to obtain the phthalein compound.
[0032] The preparation process of the rice culture medium described in step (1) is as follows: Weigh 45g of pearl rice, add 0.1gNaCl and 150mL of water into a 1L Erlenmeyer flask, stir evenly until the NaCl is completely dissolved, then sterilize at 11...
Embodiment 2
[0040] A method for extracting phthalein compounds, comprising the following steps:
[0041] (1) Cultivate Pseudomonas genus in rice culture medium, then soak with methanol for 30 hours, filter, and get the filtrate to obtain a crude extract for subsequent use;
[0042] (2) Extract the crude extract prepared in step (1) with ethyl acetate, collect the ethyl acetate phase, concentrate and dry under reduced pressure with a rotary evaporator to obtain the extract;
[0043] (3) The extract prepared in step (2) is separated by column chromatography, then separated by high performance liquid chromatography, concentrated by a rotary evaporator under reduced pressure, and then dried in a vacuum oven to obtain the phthalein compound.
[0044] The culture medium described in the step (1) is a rice culture medium, and the preparation process of the rice culture medium is as follows: take 50g pearl rice, add 0.5gNaCl and 200mL water in a 1L Erlenmeyer flask, stir until the NaCl is complet...
Embodiment 3
[0052] A method for extracting phthalein compounds, comprising the following steps:
[0053] (1) Cultivate Pseudomonas genus in culture medium, then soak for 50 hours with an organic solvent, filter, get the filtrate, promptly make the crude extract, and set aside;
[0054] (2) Extract the crude extract prepared in step (1) with ethyl acetate, collect the ethyl acetate phase, concentrate and dry under reduced pressure with a rotary evaporator to obtain the extract;
[0055] (3) The extract prepared in step (2) is separated by column chromatography, then separated by high performance liquid chromatography, concentrated by a rotary evaporator under reduced pressure, and then dried in a vacuum oven to obtain the phthalein compound.
[0056] The culture medium described in step (1) is a rice culture medium, and the preparation process of the rice culture medium is as follows: take 55g of pearl rice, add 0.5gNaCl and 250mL of water in the Erlenmeyer flask, stir until the NaCl is co...
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