Serum-free, animal-derived-component-free culture medium with definite components and application thereof

Abstract

The invention provides a serum-free, animal-derived-component-free culture medium with definite components and application thereof, and relates to the technical field of cell culture. The serum-free and animal-derived-component-free culture medium with definite components comprises a basic culture medium and cytokines, hormones, transferrin, albumin, fetal globulin, putrescine and serotonin whichare added into the basic culture medium. According to the serum-free, animal-derived-component-free culture medium with definite components, substances such as cytokines, hormones and the like are added into a basic culture medium to replace serum, so that the culture of human cells is realized. The components of the culture medium are definite, no serum and animal-derived-component components areadded, so that the technical problems of poor repeatability of the traditional serum-containing culture medium for cell culture, low cell activity and low cell amplification efficiency of other serum-free culture mediums can be mitigated.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a serum-free, animal-source component-free medium with defined components and an application thereof. Background technique [0002] Cell culture technology is an important and commonly used technology in cell biology research methods. By simulating the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro, the cells can survive, grow, reproduce and maintain the main structure and function. Obtain a large number of cells to study cell signal transduction, anabolism, growth and proliferation, etc. Of all cell cultures in vitro, the most difficult is human cell culture. [0003] The nutrients required for in vitro cell culture are basically the same as those in the body. For example, sugars, amino acids, inorganic salts, growth-promoting factors, trace elements, etc. are required. The above-mentioned substances required by ...

AI Technical Summary

Problems solved by technology

Although the traditional culture medium added with serum and other animal-derived components can realize the in vitro culture of human cells, there are substances that damage cell growth and reproduction, such as complement, immunoglobulin and some growth inhibitory factors, and the components are not clear; traditional culture The reproducibility of the base for cell culture is also poor, which affects the safety and stability of clinical (human) application; and the serum activity of different animals and different batches is quite different, which affects the stability of the culture effect; at the same time, containing Cell viability and cell expansion efficiency are low in serum-based traditional media

In order to avoid the above-mentioned problems caused by the traditional culture medium, some scholars have developed a serum-free medium by adding a defined substance to the medium instead of serum. Although these serum-free medium solve the problem of traditional medium for human cell culture The problem of poor reproducibility has improved the safety and stability of clinical applications, but the cells obtained from it still have the problems of low cell viability and low cell expansion efficiency

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