Liquid quality analysis method for detecting catecholamine content in 24H urine
A catecholamine and analysis method technology, which is applied in the field of detecting the content of neurological substances catecholamine in 24H urine, can solve the problems of easy oxidation, poor activity and stability of catecholamine substance, and difficulty in accurate determination of catecholamine concentration.
Pending Publication Date: 2019-07-02
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology
Due to the extremely low content of catecholamines in biological samples, most literatures report that the content of catecholamines in actual biological samples is extremely small, the stability of the activity of catecholamines is extremely poor, and they are easily oxidized. In addition, various structural similarities exist in biological samples and / or endogenous chemical interferents of metabolites with the same chemical group, leading to difficulties in the precise determination of catecholamine concentrations in biological samples
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[0050] The liquid mass analysis method of detecting catecholamine content in 24H urine of the present invention comprises the following steps:
[0051] (1) Calibration of standard solution
[0052] (a) Preparation of standard working solution:
[0053] Accurately weigh 3.2 mg of norepinephrine standard substance and place it in a 2mL volumetric flask, dissolve it with 0.04% formic acid aqueous solution to obtain standard stock solution A, the concentration of its norepinephrine is 1324.5 μg / mL, and dissolve it with 0.04% formic acid The aqueous solution was diluted from the standard stock solution A to obtain the standard intermediate solution B, and its norepinephrine concentration was 32 μg / mL; accurately weighed 2.80 mg of the adrenaline standard substance and placed it in a 2 mL volumetric flask, and dissolved it with 0.04% formic acid aqueous solution to obtain Prepare standard stock solution C, whose adrenaline concentration is 1400 μg / mL, dilute with 0.04% formic acid ...
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Abstract
The invention provides a liquid quality analysis method for detecting the catecholamine content in 24H urine to calibrate a standard solution by using a high performance liquid chromatography triple quadrupole tandem mass spectrometer. Standard curve equations y1=a*x1+b, y2=c*x2+d and Y3=e*x3+f are fit. After the urine to be tested is treated, the urine to be tested is detected by the high performance liquid chromatography triple quadrupole tandem mass spectrometer. The y1, y2 and y3 values of the urine to be tested are substituted into the standard curve equations. The relative concentrationsx1, x2 and x3 of three substances of catecholamine in the urine sample to be tested are calculated. The concentration of an internal standard in the standard internal standard solution is known. Theconcentration of three substances of catecholamine in the urine to be tested is calculated. The method provided by the invention has the advantages of simple and rapid detection process and high detection sensitivity, and is more favorable for measuring the content of catecholamine in a patient in clinical treatment.
Description
technical field [0001] The invention relates to the technical field of clinical content concentration monitoring of nerve substances, in particular to a method for detecting the content of nerve substance catecholamines in 24H urine. Background technique [0002] Pheochromocytoma originates from the adrenal medulla, sympathetic ganglion, and parasympathetic ganglion can secrete excess catecholamines (norepinephrine, epinephrine, dopamine, the specific chemical structure is as follows figure 1 , figure 2 , image 3 shown) and tumors in chromaffin tissues such as the adrenal gland, bladder, abdominal aorta, retroperitoneum, and heart. Because the tumor tissue can secrete a large amount of catecholamines paroxysmally or continuously, clinical symptoms of paroxysmal or persistent hypertension often appear, and the manifestations are diversified. There are also potential connections. The condition of pheochromocytoma is dangerous and changes drastically. If it is not treated...
Claims
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Login to View More IPC IPC(8): G01N30/02G01N30/72G01N30/04G01N30/06G01N30/88
CPCG01N30/02G01N30/04G01N30/06G01N30/72G01N30/88G01N2030/045G01N2030/062G01N2030/8813
Inventor 刘春冉翟瑞雪贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD