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High-yield and high-efficiency cultivation method of French Armillaria

A cultivation method, the technology of Armillaria armillaria, which is applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems that there are no public reports on artificially cultivated fruiting bodies of Armillaria armillaria, and achieve the effect of increasing the amount of mushrooms

Active Publication Date: 2021-10-15
彝良县天麻产业开发中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Armillaria fragrans is a fungus of the genus Armillaria that can be used as Gastrodia elata and Polyporus genus for planting. Its fruiting body is mainly obtained from the wild. There is no public report on the artificial cultivation of Armillaria armillaria.

Method used

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  • High-yield and high-efficiency cultivation method of French Armillaria
  • High-yield and high-efficiency cultivation method of French Armillaria
  • High-yield and high-efficiency cultivation method of French Armillaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The acquisition of embodiment 1 bacterial strain, strain identification

[0031] 1.1 Strain isolation

[0032] Two strains of Armillaria armillaria were isolated from wild Gastrodia elata (gastrodia elata tubers with fungal cords) in Xiaocaoba Town, Yiliang County, Yunnan Province, numbered AM1 and AM4. The specific separation method is as follows:

[0033] Wash the soil on Gastrodia elata with clean water, rinse with sterile water 3 times after running tap water for 15 minutes, soak in 0.1% mercuric solution for 1-2 minutes, rinse with sterile water 2-3 times, remove residual disinfectant, and then use sterile Cut out the required part of the tissue with a sterile knife and place it in a sterile petri dish, absorb the surface moisture with sterile filter paper, put it on a petri dish with PDA medium added with chloramphenicol, and start cultivating at a constant temperature of 25°C for 5 days Grow new strands.

[0034] The morphological characteristics of the above-...

Embodiment 2

[0052] Embodiment 2 Gastrodia elata cultivation test

[0053] This example examines the influence of French Armillaria gallica Xiaocaoba No. 1 (CGMCC NO.16781) on the yield and quality of Gastrodia elata as companion planting. For ease of description, the strain is still coded as AM1.

[0054] Taking Armillaria AM2, AM3, and AM4 described in Example 1 as controls, the above four strains were subjected to the concomitant planting experiment of Gastrodia elata.

[0055] Source of Gastrodia elata planting: the first generation of Gastrodia elata locally produced in Xiaocaoba, Yiliang County; cultivation location: Gastrodia elata demonstration garden in Xiaocaoba, 2010m above sea level, 104°12′2″E, 27°45′21″N. The cultivation method of "three nests" is adopted, that is, the method of placing the cultivation holes at the same time by using tree sticks, different Armillaria strains, and Gastrodia elata seeds, and the cultivation was carried out on March 20, 2017; the cultivation ho...

Embodiment 3

[0062] Embodiment 3 Biological properties compare

[0063] This example examines the biological properties of Armillaria gallica Xiaocaoba No. 1 (CGMCC NO.16781). For ease of description, the strain is still coded as AM1.

[0064] Armillaria AM2 described in Example 1 was used as a control.

[0065] The improved solid PDA medium was quantitatively inoculated with young fungal cords of strains AM1 and AM2, cultured in the dark at 25°C, and the experimental data were regularly observed and recorded.

[0066] Determination of growth

[0067] From the fourth day of inoculation, the growth amount of the strain was measured by the cross method, and was measured every day until it was overgrown.

[0068] Extracellular enzyme activity assay

[0069] Preparation of crude enzyme solution

[0070] Fill a 2.0mL centrifuge tube with the culture medium around the mycelium at random with an inoculation needle, centrifuge at 12000r / min for 15min, suck out 1mL of the supernatant and distill...

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Abstract

The invention relates to the technical field of Armillaria armillaria cultivation, in particular to a high-yield and high-efficiency cultivation method for Armillaria armillaria. The method is to inoculate Armillaria gallica Xiaocaoba No. 1 (CGMCC NO.16781) in the culture medium of the cultivation bag, and put the culture medium and sweet potatoes into the cultivation bed after the mycelium is overgrown. Inside, it can be cultivated after covering with soil. The invention firstly realizes the stable fruiting of fruiting body of Armillaria fragrans artificially cultivated, can increase the fruiting amount, and can be popularized and applied in industrialization.

Description

technical field [0001] The invention relates to the technical field of Armillaria armillaria cultivation, in particular to a cultivation method of Armillaria Armillaria Xiaocaoba No. 1 in France. Background technique [0002] Armillaria gallica (Armillaria gallica) is a fungus belonging to the genus Armillaria, which belongs to the genus Basidiomycotina, Agaricaceae, and Armillaria. ,full of nutrition. Armillaria armillaria is a wild fungus that grows on the roots of coniferous and broad-leaved trees. The growth period is from August to September every year. It is distributed in Jilin, Liaoning, Hebei, Shanxi, Gansu, Qinghai, and Sichuan. At present, it is naturally occurring in the wild woodland and picked in disorder, the natural yield is low, and its wild resources are decreasing day by day. Armillaria fragrans is a fungus of the genus Armillaria that can be planted as Gastrodia elata and Polyporus umbilicalis at present. Its fruiting body is mainly obtained from the wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/20A01G18/00
CPCA01G18/20A01G18/00A01G22/25
Inventor 王忠巧刘朝斌孙友强徐万雷李才汪唐静
Owner 彝良县天麻产业开发中心
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