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Aspergillus strain and application thereof

A technology of Aspergillus fungi and strains, applied in the fields of application, fungi, biocides, etc., can solve 3R problems, achieve rapid growth, large spore production, and simple operation process

Active Publication Date: 2019-07-05
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the future, the areas invaded by RIFA will increase rapidly, which will inevitably lead to an increase in the use of chemical agents, but traditional chemical control will lead to serious 3R problems

Method used

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  • Aspergillus strain and application thereof
  • Aspergillus strain and application thereof
  • Aspergillus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] ——Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) Acquisition, identification and preservation of ANHHYSFJ01.

[0066] (1) Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) Acquisition and identification of ANHHYSFJ01.

[0067] The fungus of the genus Aspergillus described in the present invention is isolated from red fire ants, which are collected from Dongsanhuan Road, Kunming City, Yunnan Province, Panlong District, Chenggong District, Dongchuan District, Mangshi, Dehong Dai and Jingpo Autonomous Prefecture. The complete nests with RIFA queens were reared in the laboratory.

[0068] Wash the red imported fire ants with a 75% ethanol solution for 5 seconds, then disinfect the surface of the ants with a 0.1% mercury solution for 2-3 minutes, slowly rinse with sterile water for 3 times, and use sterilized The tweezers separate the body of the RIFA according to different parts of the head, thorax and abdomen, and carefully place the different b...

Embodiment 2

[0080] ——Jifeng Aspergillus (Aspergillus genus) Aspergillus nomius ) ANHHYSFJ01 to prepare biocontrol agents.

[0081] (1) Preparation of spore suspension.

[0082] First, the Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) ANHHYSFJ01 strain was inoculated on ordinary PDA medium, and cultured in a 25°C ± incubator for 15 days at a constant temperature.

[0083] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0084] Sterilized sterile water was then added with 0.05% Tween-80.

[0085] Rinse the spores from the Petri dish with sterile aqueous solution.

[0086] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.

[0087] Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells.

[0088] Finally ...

Embodiment 3

[0092] ——Jifeng Aspergillus (Aspergillus genus) Aspergillus nomius ) ANHHYSFJ01 to prepare biocontrol agents.

[0093] (1) Preparation of spore suspension.

[0094] First, the Aspergillus genus Aspergillus jifeng ( Aspergillus nomius ) ANHHYSFJ01 strain was inoculated on ordinary PDA medium and cultured in a 26°C incubator for 15 days.

[0095] The components of the PDA solid medium are preferably in g / L: 200 g of potatoes, 20 g of glucose, 20 g of agar, and a natural pH.

[0096] Sterilized sterile water was then added with 0.05% Tween-80.

[0097] Rinse the spores from the Petri dish with sterile aqueous solution.

[0098] Pour the bacteria solution and spores in the petri dish into the cup and shake it for 30 minutes, filter it with a lens tissue, and finally get the uncounted spore suspension.

[0099] Take out the hemocytometer, place it under the microscope, and record the number of all spores in the inner 400 cells.

[0100] Finally calculate the total spore numbe...

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Abstract

The invention discloses an Aspergillus strain and an application thereof. The Aspergillus strain is named as Aspergillus nomius in a classified manner and collected in a China General MicrobiologicalCulture Collection Center (CGMCC) on December 28, 2018, the collection number of the strain is CGMCC No. 17066, and Aspergillus belongs to kingdom fungi, Eumycota, hyphomycetes, hyphomycetales, Moliliaceae and aspergilli. The Aspergillus strain is applied to preparation of biological agents for biological control of red imported fire ants.

Description

technical field [0001] The invention belongs to the technical field of microorganism isolation and cultivation, and in particular relates to an Aspergillus fungus strain and application thereof. Background technique [0002] On September 23, 2004, the Plant Quarantine Station of Wuchuan City reported the serious damage of the local red imported fire ants. On January 17, 2005, my country added RIFA as an inspection pest of imported plants, a national plant quarantine pest, a national forestry quarantine pest, and a national forestry dangerous pest. [0003] The rapid invasion of red imported fire ants is due to the strong fecundity and attack ability, and the rapid population growth leads to high density of ant nests. The red imported imported imported imported imported imported imported imported imported imported imported imported imported imported imported fire ants originated from South America. The adults have a variety of food habits. The red imported imported imported ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/66
CPCC12N1/14C12R2001/66C12N1/145A01N63/30
Inventor 马焕成伍建榕洪永生魏玉倩马翔梁倩刘丽龙娟
Owner SOUTHWEST FORESTRY UNIVERSITY
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