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Preparation method of gelidium amanisi polysaccharide

A technology of agaricus polysaccharides and agaricus polysaccharides, which is applied in the field of preparation of agaricus polysaccharides, can solve the problems of poor polysaccharide quality, high temperature, and long time consumption, and achieve the effects of shortening extraction time, reducing protein content, and high efficiency

Inactive Publication Date: 2019-07-12
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of polysaccharides from Geliflower mostly depends on the method of water extraction and alcohol precipitation, which takes a long time and high temperature, which is easy to cause the degradation of polysaccharides, and the quality of the extracted polysaccharides is far inferior to that of new extraction methods such as enzymatic methods

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  • Preparation method of gelidium amanisi polysaccharide
  • Preparation method of gelidium amanisi polysaccharide
  • Preparation method of gelidium amanisi polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Embodiment 1 compound enzymatic extraction method

[0062] The ratio of solid to liquid, the amount of compound enzyme added, and the extraction temperature were selected as single factor experiments; the amount of enzyme added, the amount of extracted enzyme, and the ratio of solid to liquid were selected as the investigation factors in the Box-Behnken test, and the yield of polysaccharide was used as the response value. Through response surface analysis ( Table 3), to determine the optimal process parameters for the extraction of polysaccharides from Geliflower by compound enzymatic method.

[0063] Table 3 Box-Benhnken design scheme of compound enzyme method and polysaccharide yield

[0064]

[0065] According to the response surface analysis in Table 3, the ratio of solid to liquid is 1:30~35 (m / v), the amount of compound enzyme (papain: cellulase=2:1) ​​is 2.0~2.5%, and the extraction temperature is 55~65℃, the extraction time is 2~2.5h, at this time, the yield...

Embodiment 2

[0067] The method for preparing polysaccharides from agaricus using complex enzymes provided by this embodiment comprises the following steps:

[0068] (1) Weigh 5g of agaricus powder, add water according to the ratio of 1:32 (m / v) to liquid and preheat at 50°C for 10 minutes, then add 2.3% compound enzyme (papain: cellulase = 2:1) in Extract at 60°C for 2 hours, and inactivate the enzyme in a boiling water bath for 15 minutes;

[0069] (2) Centrifuge at 4000r / min for 10 minutes to discard the filter residue, and rotary evaporate to 1 / 3 of the original volume;

[0070] (3) Add 4 times the volume of absolute ethanol, and alcohol precipitation at 4°C for 12 hours;

[0071] (4) Centrifuge at 4500r / min for 10 minutes, discard the supernatant, wash the precipitate three times with absolute ethanol, acetone, and ether, and then freeze-dry to obtain Geliflower polysaccharide.

[0072] When using the complex enzymatic method of this example to extract the polysaccharides from Glycyr...

Embodiment 3

[0077] The method for preparing polysaccharides from agaricus using complex enzymes provided by this embodiment comprises the following steps:

[0078] (1) Weigh 5g of agaricus powder, add water according to 1:30 (m / v) ratio of solid to liquid and preheat at 50°C for 10 minutes, then add 2.0% compound enzyme (papain: cellulase = 2:1) in Extract at 60°C for 2.0 hours, and inactivate the enzyme in a boiling water bath for 15 minutes;

[0079] (2) Centrifuge at 4500r / min for 20 minutes to discard the filter residue, and rotary evaporate to 1 / 2 of the original volume;

[0080] (3) Add 3 times the volume of absolute ethanol, and ethanol precipitation at 4°C for 8 hours;

[0081] (4) Centrifuge at 4500r / min for 20 minutes, discard the supernatant, wash the precipitate three times with absolute ethanol, acetone, and ether, and then freeze-dry to obtain the polysaccharide of Geliflower.

[0082] When using the compound enzyme method of this example to extract the polysaccharides fro...

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Abstract

The invention discloses a preparation method of gelidium amanisi polysaccharide. The preparation method comprises the following steps: (1) pre-treating a dry gelidium amanisi product and superfinely grinding and screening the dry gelidium amanisi product to obtain a gelidium amanisi powder; (2) adding water into the gelidium amanisi powder, adding a compound enzyme of papain and cellulose after preheating, and carrying out enzyme deactivation treatment after enzymolysis; (3) carrying out centrifugalization after enzyme deactivation, abandoning residues, and rotatably concentrating a supernate;(4) adding absolute ethyl alcohol into the rotary concentrated product for alcohol precipitation; and (5) carrying out centrifugalization after alcohol precipitation, abandoning the supernate, and washing the precipitate and freezing and drying the precipitate to obtain the gelidium amanisi polysaccharide. The method is short in production period, high in efficiency and hard to degrade polysaccharide, and the obtained gelidium amanisi polysaccharide is good in quality.

Description

technical field [0001] The invention belongs to the technical field of agaricus, in particular to a preparation method of agaricus polysaccharide. Background technique [0002] Geliphyllum, also known as sea jelly, red silk, and phoenix tail, is one of the important economic red algae in China. It grows on sea rocks near and below the tide line of the main dry and 6-10m below. It is common in the coasts of the Yellow Sea and Bohai Sea, Zhejiang, Fujian, and Taiwan It also grows along the coast. The polysaccharides contained in agaricus have been proven to have biological activities such as antibacterial, anti-oxidation, and anti-coagulation, and have extremely high "medicine and food" value. [0003] Among the various biologically active substances contained in Gemweed, Gemweed polysaccharides are high in content, widely used, and highly available, and are especially concerned by researchers. As the first step in the research and utilization of Gemweed polysaccharides, the ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 翟红蕾杨贤庆李来好戚勃裴若楠林婉玲郝淑贤黄卉
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI