Application of apolipoprotein D (ApoD) in preparation of Parkinson's disease medicament, ApoD preparation and preparation method thereof

A Parkinson's disease and drug technology, applied in the field of apolipoprotein D drug research, can solve the problems of less drug development and underutilized medicinal value, etc., to improve the targeting of brain tissue, prolong the circulation time in the body, The effect of improving sustained release properties

Active Publication Date: 2019-07-16
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is still little drug development for ApoD, and its medicinal value has not been fully utilized
At present, no research has found the application of ApoD as an acti

Method used

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  • Application of apolipoprotein D (ApoD) in preparation of Parkinson's disease medicament, ApoD preparation and preparation method thereof
  • Application of apolipoprotein D (ApoD) in preparation of Parkinson's disease medicament, ApoD preparation and preparation method thereof
  • Application of apolipoprotein D (ApoD) in preparation of Parkinson's disease medicament, ApoD preparation and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0035] The present embodiment provides a kind of preparation method of the ApoD oral preparation for the treatment of Parkinson's disease, and its steps are as follows:

[0036] 1. Preparation of inner layer liposomes:

[0037] Take 30mmol·L-1 lecithin and 45mmol·L-1 cholesterol ethanol solution, evaporate under reduced pressure in rotary motion, form a phospholipid bilayer film on the inner surface of the container, then add ApoD, protease inhibitor PMSF, ether solution, and conduct ultrasonic Stir for 12 minutes at a temperature of 16° C. to obtain a liposome solution; the dosage of ApoD is 10-500 mg / ml. The concentration of protease inhibitor PMSF is 1-100ug / ml. Then, slowly inject the PEG aqueous solution with a syringe while stirring in the liposome solution, the injection speed is 1ml / min, finally obtain liposome I, carry out freeze-drying process to obtain liposome I freeze-dried powder.

[0038] 2. Immunomodification of inner liposomes:

[0039]The transferrin recep...

Embodiment 2

[0044] The present embodiment provides a kind of preparation method for the oral preparation of ApoD for the treatment of Parkinson's disease, the experimental steps are as follows:

[0045] 1. Preparation of inner layer liposomes:

[0046] Evaporate an equal volume of 35mmol·L-1 lecithin and 40mmol·L-1 cholesterol in ethanol under reduced pressure to form a phospholipid bilayer film on the inner surface of the container, then add an appropriate amount of ApoD, protease inhibitor PMSF, and ether solution , ultrasonically stirred for 15 min at a temperature of 10° C. to obtain a liposome solution. Then, slowly inject the PEG aqueous solution with a syringe while stirring in the liposome solution, the injection speed is 1.5ml / min, finally obtain liposome I, carry out freeze-drying process to obtain liposome I freeze-dried powder.

[0047] 2. Immunomodification of inner liposomes:

[0048] The transferrin receptor antibody OX26 was subjected to thiol treatment, and the method w...

Embodiment 3

[0053] The present embodiment provides a kind of preparation method of the ApoD oral preparation for the treatment of Parkinson's disease, and its steps are as follows:

[0054] 1. Preparation of inner layer liposomes:

[0055] Take 50mmol·L-1 lecithin and 30mmol·L-1 cholesterol ethanol solution, and evaporate under reduced pressure by rotating to form a phospholipid bilayer film on the inner surface of the container; add ApoD, protease inhibitor PMSF, ether solution, and carry out ultrasonic stirring 10min, the temperature is 20 ℃, obtain liposome solution; Next, slowly inject PEG aqueous solution with syringe while stirring into liposome solution, injection speed is 2ml / min, finally obtain liposome I, carry out freeze-drying treatment to obtain Liposome I lyophilized powder. Other conditions are identical with embodiment 1.

[0056] 2. Immunomodification of inner liposomes:

[0057] The transferrin receptor antibody OX26 was subjected to thiol treatment, and the method wa...

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Abstract

The invention discloses application of apolipoprotein D (ApoD) in the preparation of a Parkinson's disease medicament, an ApoD oral preparation and a preparation method thereof. The preparation methodof the ApoD oral preparation comprises the following steps: S1, preparation of an inner-layer liposome: mixing liposome raw materials, carrying out rotary decompression evaporation and blow-drying, adding ApoD, auxiliary materials and ether solution, and carrying out ultrasonic stirring to obtain the liposome I; S2, modification of the liposome: adding transferrin receptor antibody OX26 subjectedto sulfhydrylation treatment into the liposome I, carrying out coupling reaction, connecting the OX26 antibody to the liposome I so as to obtain liposome II, and carrying out chromatography treatment; and S3, combining the outer layer of the liposome II with a chitosan layer to obtain composite liposome III. The invention discloses the application of the ApoD in the preparation of the Parkinson'sdisease medicament for the first time, develops the application value of the ApoD in the Parkinson's disease medicament field, and provides a brand-new Parkinson's disease medicament type-ApoD oral preparation. The preparation has good targeting effect, is not easy to degrade, and has long drug effect duration.

Description

technical field [0001] The invention relates to the field of drug research on apolipoprotein D, in particular to the application of ApoD in the preparation of Parkinson's disease medicine, the ApoD preparation and the preparation method thereof. Background technique [0002] Parkinson's disease and Parkinson's syndrome (Parkinson, PD) is a common clinical disease, is a kind of senile degenerative encephalopathy, is a degenerative disease of the central nervous system with progressive motor dysfunction as the main clinical manifestation, tremor, stiffness and movement Reduction is its most important clinical manifestation. While the prevalence of the disease is rapidly increasing, but its cure rate is low. However, there are many types of ApoD drugs currently on the market, either with poor curative effect or certain adverse reactions, and people urgently need to develop better therapeutic drugs. [0003] ApoD, namely apolipoprotein D, is very different in structure from ot...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K47/69A61K47/68A61P25/16
CPCA61K38/1709A61K47/6913A61P25/16
Inventor 阎春玲姜宏杜希恂焦倩
Owner QINGDAO UNIV
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