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White blood cell classification reagent and method

A white blood cell classification and reagent technology, applied in the field of blood analysis, can solve the problems of dependence on imports, high use costs, and inability to meet the normal use of small and medium-sized hospitals in a timely manner, so as to avoid misdiagnosis, improve accuracy, and simple and easy measurement methods.

Inactive Publication Date: 2019-07-19
SONOSCAPE MEDICAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above reagents or kits that can detect immature white blood cells all need fluorescent dyes to stain the cellular nucleic acid. Use on certain hematology analyzers capable of detecting fluorescent signals has considerable limitations

Method used

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  • White blood cell classification reagent and method
  • White blood cell classification reagent and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057]

[0058] Adjust the pH to 6.0 and the osmotic pressure to 800mOsm / kg with the reagents configured according to the above formula.

[0059] Under the condition that the temperature was kept at 35° C., 0.7 mL of the above reagent was added to 20 μL of blood sample containing immature leukocytes. Detection of leukocytes by electrical impedance and laser detection was started after 20 seconds of mixing. The volume information of white blood cells is measured by electrical impedance method, and the complexity characteristic information of white blood cells is measured by high-angle laser scattered light with a measuring angle of 8-20 degrees.

[0060] The result is as figure 1 As shown, Neu indicates neutrophils (plus basophils), Lym indicates lymphocytes, Mon indicates monocytes, Eos indicates eosinophils, and Naive indicates immature leukocytes. White blood cells are divided into five types: neutrophils (plus basophils), lymphocytes, monocytes, eosinophils, and immatu...

Embodiment 2

[0062]

[0063] Adjust the pH to 7.0 and the osmotic pressure to 1000mOsm / kg with the reagents configured according to the above formula.

[0064] Adopt embodiment 1 method to detect the leukocyte in the blood sample, the result is as follows figure 2 shown. The results showed that white blood cells were divided into five categories: neutrophils (plus basophils), lymphocytes, monocytes, eosinophils, and immature leukocytes, among which immature leukocytes were grouped separately with clear boundaries.

Embodiment 3

[0066]

[0067] Adjust the pH to 8.0 and the osmotic pressure to 850mOsm / kg with the reagent configured above.

[0068] Adopt embodiment 1 method to detect the leukocyte in the blood sample, the result is as follows image 3 shown. The results showed that white blood cells were divided into five categories: neutrophils (plus basophils), lymphocytes, monocytes, eosinophils, and immature leukocytes, among which immature leukocytes were grouped separately with clear boundaries.

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Abstract

The invention relates to the technical field of blood analysis, in particular to a white blood cell classification reagent and method. The white blood cell classification reagent is prepared from thecomponents in parts by weight: 0.1-5 parts of a zwitterionic surfactant, 0.5-10 parts of a nonionic surfactant, 0.1-30 parts of a cosolvent, 5-30 parts of an osmotic pressure regulator, 0.1-2 parts ofa preservative, 5-20 parts of a buffer agent, 0.001-0.2 part of dye and 0.1-2 parts of a fixing agent. The white blood cell classification reagent can carry out quartered grouping (4 groups corresponding to neutrophils and basophils, lymphocytes, monocytes and eosinophils) on normal blood samples and can further carry out detection on blood samples containing juvenile white blood cells of patients with specific diseases, and the blood samples of the patients can be divided into 5 groups in total which are the 4 groups corresponding to neutrophils and basophils, lymphocytes, monocytes and eosinophils and a group corresponding to the juvenile white blood cells.

Description

technical field [0001] The invention relates to the technical field of blood analysis, in particular to a white blood cell classification reagent and method. Background technique [0002] For normal healthy people, the immature white blood cells before the bone marrow cells will not appear in the peripheral blood, and there are few rod-shaped nuclear cells. However, under special circumstances, such as leukemia and other blood diseases, bone marrow cancer metastasis, and severe infection, immature white blood cells often appear. Therefore, the determination of immature white blood cells is of great significance for the diagnosis of diseases. [0003] Chinese patent CN1095481A discloses a method for classifying and counting immature white blood cells. This patent uses a hemolytic agent containing non-ionic surfactants and amino acids. The pH of the liquid is 5.0-9.0, the osmotic pressure is 150-600mOsm / kg, and the conductivity is 6.0 - 9.0 mS / cm of buffer. After treatment ...

Claims

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Application Information

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IPC IPC(8): G01N15/10
CPCG01N15/10G01N15/1031
Inventor 王泽巩刘兵
Owner SONOSCAPE MEDICAL CORP
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