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Preparation method of RNA triple helix hydrogel for targeted therapy of triple negative breast cancer

A triple-negative breast cancer, targeted therapy technology, applied in the field of biochemical nanomaterials, can solve the problems of poor prognosis, difficulty in controlling mortality and metastasis tendency, unsatisfactory treatment effect, etc., and achieve good biocompatibility

Active Publication Date: 2021-08-17
LINYI UNIVERSITY
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  • Application Information

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Problems solved by technology

Clinical studies have proved that TNBCs are the most aggressive type of breast cancer compared with other types of breast cancer, with poor prognosis, mortality and metastasis tendency are more difficult to control
Traditional therapy includes hormone therapy, targeting the three receptors, but the therapeutic effect is not satisfactory

Method used

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  • Preparation method of RNA triple helix hydrogel for targeted therapy of triple negative breast cancer
  • Preparation method of RNA triple helix hydrogel for targeted therapy of triple negative breast cancer
  • Preparation method of RNA triple helix hydrogel for targeted therapy of triple negative breast cancer

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Embodiment Construction

[0044] The preparation method of the RNA triple helix hydrogel for the targeted therapy of triple-negative breast cancer, first design the linear DNA transcription template, and form the hydrogel of the pure RNA system by rolling circle transcription, and CXCR4 is designed in the linear DNA The antisense sequence of the sequence and the triple-helix antisense sequence that can carry two therapeutic genes behind it will produce the therapeutic gene CXCR4 during the transcription and replication process, and then the other two therapeutic microRNAs and the nucleic acid targeting MDA-MB-231 cells Aptamers were hybridized by the principles of Watson-Crick and Hoogsteen base pairing, and centrifuged to form RNA triple-helix hydrogel nanostructures.

[0045] The nucleotide sequence of the linear DNA transcription template is shown in SEQ ID No.1, and the 5' end is phosphorylated.

[0046] The other two therapeutic microRNAs are tumor suppressor microRNAs and oncomiR inhibitors. Prefe...

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Abstract

The invention discloses a preparation method of RNA triple helix hydrogel for targeted therapy of triple negative breast cancer. The carrier is a pure RNA system, which is formed by rolling circle transcription. During the transcription and replication process, the therapeutic gene CXCR4 is produced, and two other therapeutic microRNAs are hybridized: tumor suppressor microRNA and oncomiR inhibitor; on the linear template Complementary sequences of nucleic acid aptamers that can target MDA-MB-231 cells are designed, cholesterol is designed on the complementary sequences of the nucleic acid aptamers, and RNA hydrogel is formed after high-speed centrifugation. The RNA triple-helix hydrogel has been successfully applied to drug sustained-release systems with good biocompatibility, and the addition of the triple-helix structure enables the RNA hydrogel to deliver three therapeutic genes at the same time, which is effective for targeted cell MDA‑ MB‑231 has broad prospects in the fields of tumor cell growth inhibition and biomedicine.

Description

technical field [0001] The invention relates to the technical field of biochemical nanometer materials, in particular to a method for preparing an RNA triple-helix hydrogel for targeted therapy of triple-negative breast cancer. Background technique [0002] Breast cancer is one of the most common cancers in women, and triple-negative breast cancers (TNBCs) refer to a type of breast cancer that lacks the expression of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) type. Clinical studies have proved that TNBCs is the most aggressive type of breast cancer compared with other types of breast cancer. Its prognosis is poor, and its mortality and metastasis tend to be more difficult to control. Traditional therapy includes hormone therapy, targeting the three receptors, but the therapeutic effect is not satisfactory. Therefore, it is urgent and important to develop more effective diagnosis and combination therapy of TNBCs. ...

Claims

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Application Information

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IPC IPC(8): A61K31/7105A61K9/06A61K48/00A61P15/14A61P35/00C12N15/10
CPCA61K9/06A61K31/7105A61P15/14A61P35/00C12N15/10C12Q2531/125C12Q2521/501C12Q2521/101
Inventor 李雪梅丁来荣赵飞浪张书圣
Owner LINYI UNIVERSITY
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