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Molecular marker detection system for improvement of 1BL/1RS chromosome in 1BL/1RS translocation line wheat and application of molecular marker detection system

A molecular marker and molecular detection technology, applied in the field of molecular breeding, can solve the problems of increased water absorption of dough, decreased stability and extensibility of dough, and decreased water holding capacity.

Pending Publication Date: 2019-07-23
SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ω-rye alkali dissolves quickly after absorbing a large amount of water, which increases the water absorption of the dough and reduces the water holding capacity, resulting in a decrease in the stability and extensibility of the dough, resulting in a decrease in the baking and cooking quality of 1BL / 1RS wheat

Method used

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  • Molecular marker detection system for improvement of 1BL/1RS chromosome in 1BL/1RS translocation line wheat and application of molecular marker detection system
  • Molecular marker detection system for improvement of 1BL/1RS chromosome in 1BL/1RS translocation line wheat and application of molecular marker detection system
  • Molecular marker detection system for improvement of 1BL/1RS chromosome in 1BL/1RS translocation line wheat and application of molecular marker detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0061] Example 2. Molecular detection of 1RS and 1BS chromosome arms.

[0062] See attached figure 2 , is the molecular detection of the 1RS and 1BS chromosome arms of the present invention. In the figure: A, molecular marker AF1 / AF4; B, molecular marker O11B3 / O11B5; C, multiplex PCR of molecular marker AF1 / AF4 and O11B3 / O11B5; M, DNA molecular weight standard (D2000); 1, A94 (Shimai 15 ω-ryeine expression deletion body); 2, Baidangdong; 3, Hardzao 2018; 4, Gaoyou 503; 5, Yannong 19; 6, Baidandong×A94 F1; 7, Hardzao 2018×A94 F1 ; 8, Gaoyou 503×A94 F1; 9, Tobacco 19×A94F1; 10, ddH 2 O.

[0063] The identification of the 1RS and 1BS chromosome arms was continued throughout the process of creating the improved type II and improved type III 1BL / 1RS chromosomes. The synthetic 1RS and 1BS chromosome arm molecular markers were compared and studied, and the 1RS chromosome arm molecular markers AF1 / AF4 and the 1BS chromosome arm molecular markers O11B3 / O11B5 were determined, and o...

Embodiment 3

[0075] Example 3. Molecular detection of high-quality subunits 7+8, 13+16, 14+15 and 17+18.

[0076] See attached image 3 , is the molecular detection of the high molecular weight glutenin subunit of the present invention. In the figure, A, molecular marker ZSBy8F5 / R5; B, molecular marker ZSBy9aF1 / R3; C, molecular marker ZSBy9F2 / R2; D, molecular marker Bx14; E, molecular marker Bx17; M, DNA molecular weight standard (D2000); 1, A94 (Shimai 15 ω-rye alkali expression deletion body); 2, Baidangdong; 3, Hardzao 2018; 4, Gaoyou 503; 5, Yannong 19; 6, Baidandong×A94 F1; 7, Hardzao 2018×A94 F1; 8, Gaoyou 503×A94 F1; 9, Yannong 19×A94 F1; 10, ddH 2 O.

[0077] Wheat high molecular weight glutenin subunits (HMW-GS) are closely related to wheat quality, especially have a significant impact on bread baking quality. The introduction of high-quality high-molecular-weight glutenin subunits into the 1BL chromosome arm can make up for the poor quality of the 1BL / 1RS translocation line t...

Embodiment 4

[0095] Embodiment 4, hexaploid wheat suppresses partial homologous chromosome pairing gene ( Ph1 ) for molecular detection.

[0096] See attached Figure 4 , for the present invention ph1 molecular detection. In the figure: A, molecular marker PSR128; B, molecular marker PSR574; M, DNA molecular weight standard (D2000); 1, Pavon ph1b Mutant; 2, A94 (Shimai 15 ω-rye alkali expression deletion mutant); 3, Baidiandong; 4, Hardzao 2018; 5, Gaoyou 503; 6, Yannong 19; 7, A94×Pavon ph1b Mutant F1;8, white hard winter × Pavon ph1b Mutant F1; 9, Hard Early 2018 Pavon ph1b Mutant F1;10, high excellent 503 × Pavon ph1b Mutant F1;11, Yannong 19 × Pavon ph1b Mutant F1;12, ddH 2 O.

[0097] Sears (1977) treated Chinese spring wheat seeds with X-rays and obtained 1 chromosome arm at 5BL Ph1 Site deletions of approximately 70 Mb fragments ph1b mutant( CSph1b ). Lukaszewski (2014) transferred the Chinese spring 5B chromosome (long arm deletion containing the Ph1 locus f...

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Abstract

The invention discloses a molecular marker detection system for improvement of 1BL / 1RS chromosome in 1BL / 1RS translocation line wheat and application of the molecular marker detection system. According to the present invention, the molecular marker detection system for chromosome improvement of the 1BL / 1RS translocation line wheat is provided, and a molecular breeding technology can be used to efficiently create the new 1BL / 1RS chromosome with high yield and stress resistance of rye 1RS and without significant influence on wheat processing quality based on the system, so then the processing quality of the 1BL / 1RS translocation line wheat is improved and a new germplasm resource is provided for wheat quality breeding.

Description

technical field [0001] The invention relates to the related technical field of agriculture and forestry breeding, in particular to a molecular breeding technology related to 1BL / 1RS translocation wheat. Background technique [0002] The wheat-rye 1BL / 1RS translocation line formed by replacing the wheat 1BS chromosome arm with the 1RS chromosome arm of rye has the disease resistance genes such as leaf rust, stem rust, stripe rust and powdery mildew carried by rye 1RS, and has good fertility and adaptability. In the world wheat breeding, the 1BL / 1RS translocation line is widely used as an excellent gene resource. It is the most successful example of exogenous genes used in wheat variety improvement, and it is also the second revolution in the history of world wheat breeding after the dwarf gene. sex work. [0003] In the 1970s, my country introduced Lovrin 13, Kavkaz, Predgornia 2, Aurora, Neuzucht and other wheat varieties from Romania. Lovelin 10, Lovelin 13, Caucasus, an...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13
Inventor 李亚青李孟军张士昌周硕史占良何明琦张楠彭义峰
Owner SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI