Meyerozyma guilliermondii suspension for controlling postharvest diseases of cherry tomato fruits

A technology for cherry tomato and postharvest diseases, which is applied in the directions of yeast-containing food ingredients, microorganism-based methods, and applications, etc., can solve the serious harm to human health and environmental safety of chemical fungicides, affect the effect of disease control, and the resistance of pathogenic microorganisms. and other problems, to achieve the effect of reducing the incidence of Botrytis cinerea, cost-effective, and convenient cultivation.

Active Publication Date: 2019-07-26
ZHEJIANG UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Residues of chemical fungicides are likely to cause serious harm to human health and environmental safety, and at the same time lead to drug resistance of pathogenic microorganisms, affecting the effect of disease control

Method used

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  • Meyerozyma guilliermondii suspension for controlling postharvest diseases of cherry tomato fruits
  • Meyerozyma guilliermondii suspension for controlling postharvest diseases of cherry tomato fruits

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1, the screening of Pichia mongolica yeast:

[0027] Sea sand from the Sansha Islands in Hainan Province is selected;

[0028] The culture reagent is tiger red (Red Bengal) medium;

[0029] The cultivation and screening conditions are: weigh 10g of sea sand, add 100mL of sterile water, shake at 200r / min for 30min, draw the supernatant, and then dilute the supernatant gradient with sterile water for 10, 10 2 、10 3 、10 4 Draw 100 μL from each gradient and spread evenly on the tiger red medium plate, culture in the incubator at 28°C for 3 days, select and purify according to the colony shape, microscopic examination and PCR amplification 16s rDNA sequencing confirmed that it was yeast Afterwards, they were stored on slant planes of NYDA medium.

[0030] Finally, the strain J-SS-CX was screened out, identified as Meyerozyma guilliermondii by 16s rDNA, and its colony characteristics were as follows: round, milky white, with neat edges, smooth and wet surface. ...

Embodiment 2

[0035] Embodiment 2, the preparation method of Pichia mongolia suspension:

[0036]Pichia mongolica J-SS-CX (CGMCC NO.17240) was stored in NYDA medium at low temperature (4°C), and when activated, it was taken out and cultured in NYDA medium at 28°C for 48 hours, and then subcultured repeatedly After 2 times, use an inoculation loop to inoculate the activated Pichia monteritima into the NYDB medium, cultivate it at 200 rpm and 28°C for 24 hours, and then absorb 1 / 50 of the volume of the medium to contain 12% NaCl. In the NYDB medium (that is, bacterial solution: NYDB medium containing 12% NaCl = 1:50 volume ratio), subculture at 200rpm and 28°C for 24h, collect the culture solution, and store at 4000rpm and 4°C After centrifugation for 15 min, the cells collected by centrifugation were washed twice with sterile distilled water to remove the culture medium. Dilute and resuspend the yeast cells with sterile distilled water, use a hemocytometer to determine the concentration und...

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Abstract

The invention discloses J-SS-CX which is meyerozyma guilliermondii with under the preservation number CGMCC NO.17240. The invention also discloses a meyerozyma guilliermondii suspension for controlling postharvest diseases of cherry tomato fruits. The preparation method of the meyerozyma guilliermondii suspension comprises the steps of inoculating meyerozyma guilliermondii J-SS-CX with an activated NYDA solid culture medium into an NYDB culture medium for one generation, then inoculating it into a NYDB culture medium containing 12% NaCl for culture, washing the obtained thalli with sterile distilled water and diluting it. The yeast preparation can effectively control postharvest diseases of fruits on the premise that a chemical bactericide is not used, and is convenient to operate, good in effect, low in cost, safe and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of prevention and control of post-harvest fruit diseases, in particular to a biological fresh-keeping technology for directly inhibiting post-harvest disease of fruits. Background technique [0002] Affected by postharvest diseases, logistics technology and various abiotic factors, postharvest fruit losses are relatively serious. Among them, the infection of pathogenic fungi is the main cause of postharvest fruit rot. The common pathogenic fungi mainly include Penicillum spp., Botrytis spp. and Alternaria spp. .)Wait. According to data, nearly 80 million tons of fruits in my country are affected by post-harvest diseases and cannot be sold every year, causing economic losses of 100 billion yuan, which seriously limits the development of my country's fruit industry. [0003] At present, chemical fungicides are still the main measures to control postharvest fruit diseases in my country. Taking citrus as an e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155C12N1/16C12R1/84
CPCA23B7/155C12N1/16A23V2002/00C12R2001/84C12N1/165A23V2200/10A23V2250/76
Inventor 郑晓冬曹璇
Owner ZHEJIANG UNIV
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