Gingival cell hydrogel filling agent and preparation method and application thereof
A hydrogel and filler technology, applied in the field of cell application, can solve the problems of insignificant therapeutic effect, poor stability, and low cell survival rate, and achieve good economic benefits, good therapeutic effect, and simple operation steps
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Embodiment 1
[0037] 1. Extraction of patient's autologous gingival cells and cell culture passage:
[0038] (1) Extraction of patient's own gingival cells
[0039] Precautions for taking materials in the operating room: before deciding to take gingiva tissue, medical personnel with the qualifications of medical practitioners shall explain the reasons to the patient or the family members of the patients, and obtain the consent of the patients or family members. , and draw materials in accordance with the surgical principles stipulated by the hospital. Patients undergo physical examinations and laboratory tests, including routine tests and inspections of infectious disease pathogens (such as HIV, hepatitis virus, etc.). The taken gingiva was immediately put into a sterile container by professionals, and immediately refrigerated (below 10°C), and then processed according to the prescribed standard operating procedures.
[0040] Isolation and culture of gingival fibroblast-like cells, compri...
Embodiment 2
[0076] Other operating steps are consistent with Example 1, except that the method for constructing a bioscaffold attached with autologous gingival fibroblast-like cells specifically includes the following steps:
[0077] Use soluble medicinal chitosan as material, mix with DMEM medium to prepare a chitosan mixed solution with a concentration of 10wt%, and pre-cool it at 4°C for later use. According to the concentration of 8mg / ml, add 4*10 3 Cell / ml fibroblast-like cells were mixed at 30°C for 30 minutes, and mixed repeatedly to form a biological scaffold with autologous gingival fibroblast-like cells attached, and stored at 4°C for later use.
Embodiment 3
[0079] Other operating steps are consistent with Example 1, except that the method for constructing a bioscaffold attached with autologous gingival fibroblast-like cells specifically includes the following steps:
[0080] Use soluble medicinal chitosan as material, mix with DMEM medium to prepare a chitosan mixed solution with a concentration of 7wt%, and pre-cool it at 8°C for later use. According to the concentration of 6mg / ml, add 6*10 3 Cell / ml fibroblast-like cells were mixed at 20°C for 20 minutes, and mixed repeatedly to form a biological scaffold with autologous gingival fibroblast-like cells attached, and stored at 8°C for later use.
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