Gingival cell hydrogel filling agent and preparation method and application thereof

A hydrogel and filler technology, applied in the field of cell application, can solve the problems of insignificant therapeutic effect, poor stability, and low cell survival rate, and achieve good economic benefits, good therapeutic effect, and simple operation steps

Inactive Publication Date: 2019-08-02
张永国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the application of cell therapy in the gums found that the survival rate of the cells is not high, the stability is not good, and the therapeutic effect is not significant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Extraction of patient's autologous gingival cells and cell culture passage:

[0038] (1) Extraction of patient's own gingival cells

[0039] Precautions for taking materials in the operating room: before deciding to take gingiva tissue, medical personnel with the qualifications of medical practitioners shall explain the reasons to the patient or the family members of the patients, and obtain the consent of the patients or family members. , and draw materials in accordance with the surgical principles stipulated by the hospital. Patients undergo physical examinations and laboratory tests, including routine tests and inspections of infectious disease pathogens (such as HIV, hepatitis virus, etc.). The taken gingiva was immediately put into a sterile container by professionals, and immediately refrigerated (below 10°C), and then processed according to the prescribed standard operating procedures.

[0040] Isolation and culture of gingival fibroblast-like cells, compri...

Embodiment 2

[0076] Other operating steps are consistent with Example 1, except that the method for constructing a bioscaffold attached with autologous gingival fibroblast-like cells specifically includes the following steps:

[0077] Use soluble medicinal chitosan as material, mix with DMEM medium to prepare a chitosan mixed solution with a concentration of 10wt%, and pre-cool it at 4°C for later use. According to the concentration of 8mg / ml, add 4*10 3 Cell / ml fibroblast-like cells were mixed at 30°C for 30 minutes, and mixed repeatedly to form a biological scaffold with autologous gingival fibroblast-like cells attached, and stored at 4°C for later use.

Embodiment 3

[0079] Other operating steps are consistent with Example 1, except that the method for constructing a bioscaffold attached with autologous gingival fibroblast-like cells specifically includes the following steps:

[0080] Use soluble medicinal chitosan as material, mix with DMEM medium to prepare a chitosan mixed solution with a concentration of 7wt%, and pre-cool it at 8°C for later use. According to the concentration of 6mg / ml, add 6*10 3 Cell / ml fibroblast-like cells were mixed at 20°C for 20 minutes, and mixed repeatedly to form a biological scaffold with autologous gingival fibroblast-like cells attached, and stored at 8°C for later use.

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PUM

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Abstract

The invention provides a gingival cell hydrogel filling agent and a preparation method and application thereof. The gingival cell hydrogel filling agent adopts a water-soluble chitosan material as a holder, wherein autologous gingival fibroblasts and / or gingival mesenchymal cells are attached to the holder. The gingival cell hydrogel filling agent for injection provided by the invention is composed of autologous hypodermal cells attached to the chitosan hydrogel holder; the autologous fibroblast-like cells are obtained by performing in-vitro expansion and culture on fibroblast-like cells derived from autologous gingiva of a patient; the treatment effect is good; the stability is improved; the holder per se has the advantages that first, the attachment growth of the fibroblast-like cells can be supported; second, the holder can be absorbed; third, the holder is soft and injectable; fourth, certain temperature-sensitive properties are achieved, hydrogel is automatically formed at a bodytemperature of 37 DEG C, and therefore, the holder is worthy of being popularized and applied widely.

Description

technical field [0001] The invention relates to the field of cell application, in particular to a gingival cell hydrogel filler and its preparation method and application. Background technique [0002] With the advancement of society and the vigorous development of science and technology, human beings have higher expectations for the quality of life and life expectancy. It is everyone's dream to have a healthy, happy and happy life cycle. However, like tumors and other stubborn diseases, traditional treatment methods have entered a plateau, and the side effects brought about by them have also made many patients miserable. [0003] Cell therapy is a new technology for disease treatment that has emerged in recent years. It refers to the use of the characteristics of certain cells with specific functions, which are obtained by bioengineering methods and / or processed by in vitro expansion and special culture to enhance the ability of these cells. Immunity, killing pathogens an...

Claims

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Application Information

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IPC IPC(8): A61K35/33A61K35/28A61K9/06A61K47/36A61P1/02
CPCA61K9/0024A61K9/0063A61K9/06A61K35/28A61K35/33A61K47/36A61P1/02A61K2300/00
Inventor 张永国何君
Owner 张永国
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