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Use method of single-layer molybdenum disulfide and DNA cleaving method

A single-layer molybdenum disulfide, reaction technology, applied in the field of biomedicine

Active Publication Date: 2019-08-02
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102286458A discloses a DNA cutting method based on metal ions and graphene oxide. This patent prepares an aqueous solution of graphene oxide, and adds the sterilized graphene oxide and metal ions to the DNA buffer, and reacts at room temperature. Realize DNA cleavage; although the patent does not need to add any auxiliary reagents or use light irradiation, graphene oxide still needs to be combined with metal ions to complete DNA cleavage

Method used

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  • Use method of single-layer molybdenum disulfide and DNA cleaving method
  • Use method of single-layer molybdenum disulfide and DNA cleaving method
  • Use method of single-layer molybdenum disulfide and DNA cleaving method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for cutting DNA, the preparation method is as follows:

[0042] The monolayer molybdenum disulfide with a size of 225nm is mixed with the PBS buffer of DNA and reacted at 25°C for 18h to realize the cleavage of DNA;

[0043] Wherein, the concentration of monolayer molybdenum disulfide is 50 mg / L, the concentration of DNA is 0.015 μg / μL, the pH value of the reaction system is 7, and the DNA is pBR322.

Embodiment 2-4

[0045] The only difference from Example 1 is that in this example, the size of the monolayer molybdenum disulfide is 175nm (Example 2), 300nm (Example 3), and 325nm (Example 4).

Embodiment 5-7

[0047] The only difference from Example 1 is that in this example, the concentration of monolayer molybdenum disulfide is 10mg / L (Example 5), 100mg / L (Example 6), and 200mg / L (Example 7).

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Abstract

The invention provides a use method of single-layer molybdenum disulfide and a DNA cleaving method. The single-layer molybdenum disulfide is used for cleaving DNA. The DNA cleaving method provided bythe invention can achieve DNA cleavage at normal temperature by using only the single-layer molybdenum disulfide without adding any other auxiliary reagent.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for using monolayer molybdenum disulfide and a method for cutting DNA. Background technique [0002] Nucleic acid is an important biomacromolecule of life. It not only plays an important role in the continuation of life, the maintenance of genetic characteristics of biological species, growth and development, and cell differentiation, but also plays an important role in biological variation, such as tumors, genetic diseases, metabolic diseases, etc. are also closely related. Therefore, how to effectively manipulate nucleic acid molecules has potential application value not only in the treatment of cancer and other diseases, but also in biotechnology itself. In the early 1950s, biologists discovered the restriction and modification of DNA through the infection behavior of phages. In 1970, the first restriction endonuclease Hind II was isolated from Haemophilus influenz...

Claims

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Application Information

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IPC IPC(8): C12N15/10
CPCC12N15/10
Inventor 蒋兴宇赵樱灿徐佳仪
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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