Method for rapid dyeing and identification of zinc content in rice

A rapid dyeing and zinc content technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., to achieve the effects of wide applicability, fast identification and low cost

Pending Publication Date: 2019-08-02
FUJIAN AGRI & FORESTRY UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Purpose of the invention: A method for quick dyeing and identification of zinc content in rice is to overcome the defects of cumbersome pretreatment and expensive instrument purchase and operation required for the existing chemical quantitative detection of zinc content

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapid dyeing and identification of zinc content in rice
  • Method for rapid dyeing and identification of zinc content in rice
  • Method for rapid dyeing and identification of zinc content in rice

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1

[0031] A method for rapid dyeing and identification of zinc content in rice is based on the characteristic of forming a unique red complex (Zn-DTZ) between zinc ions (Zn) and diphenylthiocarbazone (DTZ) reagents on the surface of rice grains. The color depth of the complex can be used to determine the zinc content of rice grains by intuitive qualitative or indirect quantitative methods, that is, the darker the dyeing color, the higher the zinc content.

[0032] The rice grains in this example were derived from a comparative test of field rice varieties (A, B, C, D, E). In this example, at the maturity stage of rice, 50 grains of different rice varieties were collected in the field, and the husks were removed from the grains manually or by a rice hulling machine, and then put into 15 mL centrifuge tubes, and then 500 mg L of prepared rice was added. -1 About 10 ml of dithizone solution immerse the grains, cover the lid, shake well, soak the grains at room te...

Example Embodiment

[0033] Example 2

[0034] It is basically the same as Example 1, the difference is that the rice grains in this example come from different zinc fertilizer application methods in the field (no zinc fertilizer, soil zinc fertilizer, spray zinc fertilizer), soil zinc fertilizer treatment 50 kg / hm 1 day before rice transplanting 2 Zinc sulfate heptahydrate (ZnSO 4 ·7H 2 O) Soil application, spraying zinc fertilizer at the rate of 800 L / hm at tillering stage and 7 days after flowering respectively 2 The concentration of zinc sulfate was 0.3% for foliar spraying, and the rice grains treated with different amounts of zinc fertilizer and application methods at the harvest period were dehulled and identified by DTZ rapid staining. the result shows( figure 2 ), the effect of foliar spraying zinc fertilizer on enhancing the zinc content of rice grains was significantly higher than that of soil application, and the results of dyeing identification among different treatments were con...

Example Embodiment

[0035] Example 3

[0036] Basically the same as Examples 1 and 2, the difference is that the rice grains in this example are derived from different zinc fertilizer varieties in the field (ionic zinc fertilizer ZnSO). 4 ·7H 2 Comparison test of O, organic chelated zinc fertilizer HBED-Zn) and application methods (soil application, foliar spraying), soil application of zinc fertilizer is 50 kg / hm 1 day before rice transplanting 2 Zinc sulfate heptahydrate (ZnSO 4 ·7H 2 O) Soil application, spraying zinc fertilizer at the rate of 800 L / hm at tillering stage and 7 days after flowering respectively 2 The concentration of 0.3% zinc sulfate was used for foliar spraying, and the rice grains treated with different zinc fertilizer varieties and application methods at the harvest period were dehulled and identified by DTZ rapid dyeing. the result shows( image 3 ), the soil application of organic chelated zinc fertilizer was more effective than ionic zinc fertilizer in enhancing the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for rapid dyeing and identification of the zinc content in rice. According to the method, on the basis of the principle of forming a red complex (Zn-DTZ) by a diphenylthiocarbazone (DTZ) reagent and a zinc ion, the zinc content of rice is measured by matching the chemical analysis; a theoretical relationship model is established with diphenylthiocarbazone dyeing andidentification, so that a technical support is provided for determining the zinc content of the rice rapidly by observing the color of the red complex. The method is not limited by varieties, seasonsand experimental conditions and is operated conveniently; the identification is done rapidly; the cost is low; and the method has the high operability and applicability. With the provided method, thezinc nutrition level of rice can be estimated; and the theoretical basis and practical guidance are provided for screening the high-zinc rice and zinc-rich nutritious rice and evaluating the nutrition biological enhancement effect of the rice zinc under the zinc fertilizer optimization management reasonably.

Description

technical field [0001] The invention belongs to the technical field of agriculture and agricultural bioengineering, and specifically relates to a method for rapid dyeing and identification of zinc content in rice. Diphenylthiocarbazone (DTZ) reagents are used to form a red complex (Zn-DTZ) with zinc ions. The method for quickly judging the zinc content level of rice by the color depth of the red complex is used for screening high zinc content rice seeds and zinc-enriched nutrient rice, and reasonably evaluating the effect of zinc nutrient bioaugmentation in rice for optimal management of zinc fertilizers. Background technique [0002] Zinc (Zn) is an essential micronutrient element for plants. It is a component of more than 300 enzymes in plants. It participates in the synthesis of auxin and chlorophyll, participates in the photosynthesis of plants and the production and transport of photosynthetic compounds, and then regulates the growth and development of plants. Growth an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 郭九信王玉雯李延
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap