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Method for rapid dyeing and identification of zinc content in rice

A rapid dyeing and zinc content technology, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., to achieve the effects of wide applicability, fast identification and low cost

Pending Publication Date: 2019-08-02
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Purpose of the invention: A method for quick dyeing and identification of zinc content in rice is to overcome the defects of cumbersome pretreatment and expensive instrument purchase and operation required for the existing chemical quantitative detection of zinc content in rice. , A low-cost method for detecting zinc content in rice, providing theoretical support and practical guidance for the screening of high zinc content rice varieties, the screening of zinc-rich nutrient rice and the evaluation of rice zinc nutrient bioenhancement effects

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  • Method for rapid dyeing and identification of zinc content in rice
  • Method for rapid dyeing and identification of zinc content in rice
  • Method for rapid dyeing and identification of zinc content in rice

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A rapid staining and identification method for zinc content in rice is based on the characteristic of a unique red complex (Zn-DTZ) formed between zinc ions (Zn) on the surface of rice grains and diphenylthiocarbazone (DTZ) reagents. The color depth of the complex can be used to determine the level of zinc content in rice grains by intuitive qualitative or indirect quantitative methods, that is, the darker the dyeing color, the higher the zinc content.

[0032] The rice grains in this example are derived from the field rice varieties (A, B, C, D, E) comparative experiments. In this example, at the maturity stage of rice, 50 grains of rice of different rice varieties in the field were collected respectively, and the chaff of the rice was removed manually or by a huller, and then put into 15 mL centrifuge tubes respectively, and then added 500 mg L -1 Submerge the grains in about 10 ml of dithizone solution, cover and shake well, soak at room temperature 25±5°C for 30 min...

Embodiment 2

[0034] It is basically the same as Example 1, the difference is that the rice grains of this example are derived from the treatment experiments of different zinc fertilizer application methods in the field (no zinc fertilizer, soil zinc fertilizer, spray zinc fertilizer), soil zinc fertilizer treatment 50 kg / hm 1 day before rice transplanting 2 Zinc sulfate heptahydrate (ZnSO 4 ·7H 2 O) Soil application, spraying zinc fertilizer at 800 L / hm at the tillering stage of rice and 7 days after flowering 2 The concentration of 0.3% zinc sulfate was sprayed on the leaves, and the rice grains treated with different zinc fertilizer amounts and application methods during the harvest period were removed from the glumes and quickly stained with DTZ for identification. the result shows( figure 2 ), the effect of foliar spraying zinc fertilizer on enhancing the zinc content of rice grains was significantly higher than that of soil application, and the results of dyeing identification amo...

Embodiment 3

[0036] Substantially the same as Examples 1 and 2, the difference is that the rice grains of the present embodiment are derived from different zinc fertilizer varieties in the field (ionic state zinc fertilizer ZnSO 4 ·7H 2 O. Comparative test of organic chelated zinc fertilizer (HBED-Zn) and application methods (soil application, foliage spraying), soil application of zinc fertilizer treatment is 50 kg / hm 1 day before rice transplanting 2 Zinc sulfate heptahydrate (ZnSO 4 ·7H 2 O) Soil application, spraying zinc fertilizer at 800 L / hm at the tillering stage of rice and 7 days after flowering 2 The concentration of 0.3% zinc sulfate was sprayed on the leaves, and the rice grains treated with different zinc fertilizer varieties and application methods at the harvest period were removed from the glumes and then quickly stained with DTZ for identification. the result shows( image 3 ), the effect of soil application of organic chelated zinc fertilizer on enhancing the zinc co...

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Abstract

The invention provides a method for rapid dyeing and identification of the zinc content in rice. According to the method, on the basis of the principle of forming a red complex (Zn-DTZ) by a diphenylthiocarbazone (DTZ) reagent and a zinc ion, the zinc content of rice is measured by matching the chemical analysis; a theoretical relationship model is established with diphenylthiocarbazone dyeing andidentification, so that a technical support is provided for determining the zinc content of the rice rapidly by observing the color of the red complex. The method is not limited by varieties, seasonsand experimental conditions and is operated conveniently; the identification is done rapidly; the cost is low; and the method has the high operability and applicability. With the provided method, thezinc nutrition level of rice can be estimated; and the theoretical basis and practical guidance are provided for screening the high-zinc rice and zinc-rich nutritious rice and evaluating the nutrition biological enhancement effect of the rice zinc under the zinc fertilizer optimization management reasonably.

Description

technical field [0001] The invention belongs to the technical field of agriculture and agricultural bioengineering, and specifically relates to a method for rapid dyeing and identification of zinc content in rice. Diphenylthiocarbazone (DTZ) reagents are used to form a red complex (Zn-DTZ) with zinc ions. The method for quickly judging the zinc content level of rice by the color depth of the red complex is used for screening high zinc content rice seeds and zinc-enriched nutrient rice, and reasonably evaluating the effect of zinc nutrient bioaugmentation in rice for optimal management of zinc fertilizers. Background technique [0002] Zinc (Zn) is an essential micronutrient element for plants. It is a component of more than 300 enzymes in plants. It participates in the synthesis of auxin and chlorophyll, participates in the photosynthesis of plants and the production and transport of photosynthetic compounds, and then regulates the growth and development of plants. Growth an...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 郭九信王玉雯李延
Owner FUJIAN AGRI & FORESTRY UNIV
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