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Rapid germination method of aconitum brachypodum var.laxiflorum seeds

A seed and rapid technology, applied in the direction of seed and rhizome treatment, application, root crop cultivation, etc., can solve the problems of effective judgment of seed maturity, complicated technical method operation, complicated operation, etc., so as to avoid the pre-germination process and simplify the technology. Operation process, the effect of expanding the stock of resources

Inactive Publication Date: 2019-08-06
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, the breeding and sowing methods of Artemisia spp. involved in the prior art (CN101699945B, CN107027496A, CN108605752A) have not effectively judged the maturity of the seeds, did not describe the characteristics of the sowing substrate, and did not count the germination time, germination rate and migration. Survival rate and other indicators of planting; some technical methods are more complicated to operate, and special containers must be used to realize the sowing process of Artemisia spp.
There are still some defects in the germination method of Artemisia spp. above, and the operation is relatively complicated, which is not conducive to large-scale demonstration and promotion.

Method used

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  • Rapid germination method of aconitum brachypodum var.laxiflorum seeds
  • Rapid germination method of aconitum brachypodum var.laxiflorum seeds
  • Rapid germination method of aconitum brachypodum var.laxiflorum seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Materials and methods:

[0029] In December 2017, at the planting base of Lijiang Alpine Botanical Garden of Kunming Institute of Botany, a mature and disease-free stalk of Artemisia pods on snow was harvested in the field. After rubbing, obtain the fresh seeds of Artemisia annua on Snow, observe and screen the seeds, remove the seeds with smaller particles and lighter color (light brown), select the seeds with a diameter of about 2mm, and dark black seeds, set up the screening group and the control group respectively, press There are three repetitions in each group, 200 seeds per repetition, sowing in a 30cm*50cm*8cm seeding basket; at the same time, set the surface direct sowing and soil covering group for comparison, wherein the soil covering group is 0.5cm thick after seed sowing, sowing The baskets were placed in the greenhouse to overwinter and germinate (the daily temperature changes between 5-22°C), and water was regularly sprayed to keep them moist according...

Embodiment 2

[0035] 1. Materials and methods:

[0036]In January 2018, at the planting base of Lijiang Alpine Botanical Garden of Kunming Institute of Botany, a mature and disease-free Artemisia spp. pod was harvested in the field. After kneading, get the fresh seeds of Artemisia annua, observe and screen the seeds, remove the seeds with smaller particles and lighter color (light brown), select the seeds with a diameter of about 2mm and dark black seeds, and set the constant temperature group (20°C) and For the natural control group (10-22°C), the germination substrates were selected as mountain base soil + grass coal soil, mountain base soil + perlite, grass coal soil + perlite, mountain base soil + grass coal soil + perlite, and the matrix matched The proportions are equal volumes, and the pH value of the matrix is ​​measured at the same time. 200 seeds were sown in each group, and the thickness of the covering soil was 0.5 cm after the seeds were sown, and water was regularly sprayed t...

Embodiment 3

[0043] 1. Materials and methods:

[0044] In January 2018, at the planting base of Lijiang Alpine Botanical Garden of Kunming Institute of Botany, a mature and disease-free Artemisia spp. pod was harvested in the field. After kneading, the fresh seeds of Artemisia annua on Snow were obtained. The seeds were not screened, and they were germinated at a constant temperature of 20°C. The mixed matrix of mountain base soil + grass coal soil + perlite (the proportion of the matrix was equal volume) was selected, and different concentrations were used before germination. Soak the GA3 solution overnight (12h), set the concentration gradient to 0ppm, 50ppm, 100ppm, 150ppm, 200ppm, 250ppm, 300ppm, 400ppm, 500ppm, 200 seeds in each group, and cover the soil with a thickness of 0.5cm after the seeds are sown. The surface humidity of the substrate was sprayed with water to keep it moist, and the germination results were observed after 30 days.

[0045] 2. Results and analysis:

[0046] T...

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Abstract

The invention provides a rapid germination method of aconitum brachypodum var.laxiflorum seeds. The method comprises the steps that mature aconitum brachypodum var.laxiflorum pods in the current yearare selected, and rubbed to obtain seeds, the seeds are observed and screened, the seeds which are smaller in size and lighter in color (sandy beige) are removed, the dark black seeds with the diameters of about 2 millimeters are selected, soaked in a GA3 solution with a 250 ppm concentration for 12 hours, and sown on the surface of a seeding matrix with humic soil and black soil which are sievedby a 40-mesh sieve and uniformly mixed in a ratio of 1:1, 0.5-centimeter soil is spread for coverage, a tunnel shed is built, the seeds germinate at the normal temperature under the condition that thelowest temperature is greater than 5 DEG C, wetting of the surface of a germination matrix is maintained, the seeds root and germinate after 30-40 days, the cotyledons grow out 10 days later, and thecomprehensive seedling rate is 90% or above. Through the simple and rapid germination method of the aconitum brachypodum var.laxiflorum seeds, the resource reserve is effectively increased, and a keytechnology of the rapid germination method of the aconitum brachypodum var.laxiflorum seeds is obtained.

Description

[0001] Technical field: [0002] The invention belongs to the field of biotechnology, and in particular relates to a method for rapid germination of Artemisia spp. [0003] Background technique: [0004] Aconitum brachypodum var. laxiflorum is a variant of Aconitum brachypodum var. laxiflorum, a species of Aconitum brachypodum in the Ranunculaceae family. Mainly distributed in the northeast and northwest of Yunnan, such as Dongchuan, Huize, Xuanwei, Zhaotong, Qiaojia, Ninglang, Yongsheng, Dali, Lijiang, Zhongdian and other places, wild in alpine grasslands at an altitude of 3100-4300m, On rocky slopes or under sparse forests. [0005] Artemisia annua on the snow is a painkiller for bruises and wounds that originated in Yunnan and is widely circulated and used among the people in Sichuan. Although it has an immediate curative effect on tumbling and swelling, rheumatism, redness and swelling, especially various internal and external pains, it is very toxic, and folks often caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01G22/25
CPCA01C1/00A01G22/25
Inventor 刘维暐许琨黄华朱文浩明升平范中玉李金
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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