Bifidobacterium pseudocatenulatum CCFM1048, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1048 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1048

A bifidobacterium and fermented food technology, applied in the field of microorganisms, can solve the problems of irritating the gastrointestinal tract, liver function damage, affecting patients, etc., and achieve the effect of alleviating weight gain, alleviating metabolic syndrome, and reducing the lower area

Active Publication Date: 2019-08-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have a good curative effect on each single risk factor of metabolic syndrome. However, long-term use of drugs has certain side effects. For example, long-term use of metformin can cause irritat

Method used

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  • Bifidobacterium pseudocatenulatum CCFM1048, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1048 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1048
  • Bifidobacterium pseudocatenulatum CCFM1048, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1048 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1048
  • Bifidobacterium pseudocatenulatum CCFM1048, composition, fermented food, application and bacterial agent of bifidobacterium pseudocatenulatum CCFM1048 and preparation method of bacterial agent of bifidobacterium pseudocatenulatum CCFM1048

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1: Bifidobacterium CCFM1048 has good tolerance to simulated gastrointestinal fluid The frozen Bifidobacterium CCFM1048 is inoculated in mMRS medium (MRS medium+0.05% cysteine ​​salt In salt), anaerobic culture at 37°C for 48 hours, and subcultured by mMRS culture medium for 2 to 3 times, 1 mL of the culture liquid of Bifidobacterium pseudospermia CCFM1048 was mixed with 9.0 mL of pH 2.5 artificially simulated gastric juice (containing 1% pepsin, mMRS medium with pH=2.5) were mixed, and cultured anaerobically at 37°C, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultured with mMRS agar medium, plate colonies were counted, and determined The number of viable bacteria was counted and their survival rate was calculated.

[0085] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the time of 0h in the culture fluid, expresse...

Embodiment 2

[0090] Example 2: Bifidobacterium pseudosmall chain CCFM1048 has no toxic and side effects on C57BL / 6J mice Bifidobacterium pseudosmall chain CCFM1048 bacterium was resuspended in 3% sucrose solution to make a concentration of 3.0 × 108 CFU / mL bacteria Suspension. Get 6 healthy male C57BL / 6J mice with a body weight of about 20g. After acclimating to the environment for a week, give the bacterial suspension of this concentration orally once a day, observe for a week, and record the death and body weight.

[0091] The results of these tests are listed in Table 3. These results show that feeding concentration of 3.0 × 108CFU / mL of pseudo small chain Bifidobacterium CCFM1048 does not cause significant impact on mice, body weight does not change significantly, and no death occurs. The appearance of the mice had no obvious pathological symptoms.

[0092] Table 3 Changes in body weight and death of mice

[0093]

[0094] Note: -: no death of mice

Embodiment 3

[0095] Embodiment 3: Pseudosmall chain Bifidobacterium CCFM1048 has recovery effect to the intestinal flora disorder caused by high-fat feed

[0096] 36 healthy male C57BL / 6J mice weighing 18-20 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-fat model control group (HFD), simvastatin control group (SC ), rosiglitazone control group (RH), Bifidobacterium pseudospermia CCFM1048 intervention group (CCFM1048), Bifidobacterium animalis BB12 control group (BB12), each group contained 6 mice, and the bacteria suspension The dose is 3.0×108CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:

[0097] Table 4 Grouping of experimental animals

[0098]

[0099] The body weight of the mice was monitored regularly during the experiment.

[0100] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the...

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Abstract

The invention discloses bifidobacterium pseudocatenulatum CCFM1048, a composition, fermented food, application and bacterial agent of the bifidobacterium pseudocatenulatum CCFM1048 and a preparation method of the bacterial agent of the bifidobacterium pseudocatenulatum CCFM1048. The bifidobacterium pseudocatenulatum CCFM1048 can tolerate the gastrointestinal environment of the human body, significantly relieve the rise, caused by the high fat diet, of content of triglyceride in the liver of a mouse with the metabolic syndrome, relieve the weight rise of the mouse with the metabolic syndrome, significantly improve abnormal fasting blood glucose and abnormal oral glucose tolerance of the mouse with the metabolic syndrome, reduce the area under the drug-time curve of glucose tolerance, improve the serum insulin level, significantly relieve the rise, caused by the high fat diet, of content of low density lipoprotein cholesterol in serum and significantly relieve the rise, caused by the high fat diet, of Proteus in the intestinal tract; the bifidobacterium pseudocatenulatum CCFM1048 is used for preparing the medicine composition and the fermented food which relieve the metabolic syndrome and reduce the occurrence of diseases such as the Parkinson disease, intestinal infection and urinary tract and kidney infection and has a quite wide application prospect.

Description

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Claims

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Application Information

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Owner JIANGNAN UNIV
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