Preparation method and application of high-purity confusarin

Abstract

The invention discloses a preparation method of high-purity confusarin, which comprises the steps of cutting fresh dendrobium fimbriatum into sections, conducting homogenizing, extracting, concentrating, carrying out chromatographic separation, removing pigment and carrying out gradient elution, and confusarin with the purity of more than 95% can be obtained by the method. The invention also discloses an application of high-purity confusarin in identification of dendrobium fimbriatum, which comprises the preparation steps of a reference substance solution and a test solution and the identification step using thin-layer chromatography. According to the invention, dendrobium fimbriatum is identified in a qualitative and quantitative mode through the thin-layer chromatography, the problem ofwrong identification in a character identification method is solved, a more scientific and standard identification mode is provided for identification of the dendrobium fimbriatum, and an idea is provided for making identification standards of the dendrobium fimbriatum for industries or departments; meanwhile, the purity of the prepared confusarin can reach more than 95%, so that a support is provided for experimental research of confusarin, and a foundation is laid for industrial production of confusarin.

Description

Background technique [0001] Dendrobium is also known as Immortal Dendrobium Orchid Rhyme, Immortal Grass, Resurrection Grass, Ziying Immortal Strain, Chlorophytum, Forest Orchid, Forbidden Life, Nobile Flower, etc.; the stem is erect, fleshy and thick, slightly flattened cylindrical, 10-60 cm long , as thick as 1.3 cm. Medicinal plant, sweet and slightly salty in nature and flavor, cold, and returns to the stomach, kidney, and lung meridian. Reinforcing stomach and promoting body fluid, nourishing yin and clearing heat; used for yin injury and body fluid deficiency, dry mouth and polydipsia, retching due to lack of food, asthenia heat after illness, and blurred vision. Dendrobium is elegant, exquisite and lovely, with bright colors and fragrant smell. It is known as one of the "four major ornamental foreign flowers". [0002] Dendrobium can be subdivided into seventy or eighty different varieties according to different origins and characters, and different dendrobiums have d...

AI Technical Summary

Problems solved by technology

However, at present, the identification of Dendrobium fringe is mostly focused on the stage of character identification of Dendrobium, which is very prone to misjudgment; at the same...

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