Application of chromatin remodeling complex core catalytic subunit to preparation of drug for treating or preventing hepatitis B virus infection

A technology for hepatitis B virus and chromatin remodeling, which is used in antiviral agents, pharmaceutical formulations, peptide/protein components, etc.

Pending Publication Date: 2019-08-16
SHANGHAI CITY PUDONG NEW AREA GONGLI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research report on the relationship between BRG1 expression and HBV replication

Method used

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  • Application of chromatin remodeling complex core catalytic subunit to preparation of drug for treating or preventing hepatitis B virus infection
  • Application of chromatin remodeling complex core catalytic subunit to preparation of drug for treating or preventing hepatitis B virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0043] [Example 2] The anti-HBV activity of the core catalytic subunit protein BRG1 of the chromatin remodeling complex was evaluated by interference experiments.

[0044] The application of a chromatin remodeling complex core catalytic subunit protein BRG1 in the preparation of medicines for treating or preventing hepatitis B virus infection, the steps of which are:

[0045] 1. Experimental materials:

[0046] The interference RNA package was purchased from Guangzhou Ruibo Company.

[0047] 2. Experimental method

[0048] Same as Example 1.

[0049] 3. Experimental results:

[0050] Firstly, the interference effect of interfering RNA was detected, such as figure 1 As shown in C, the inhibition efficiency is about 80%. Such as figure 1 D, Interference BRG1 shown in 1E up-regulates the expression of HBeAg and HBsAg and the level of HBV replication intermediate nucleocapsid-related DNA in cells, indicating that BRG1 inhibits HBV replication and BRG1 has anti-HBV activity. ...

Embodiment 3

[0053] Such as figure 2 The corresponding amount of pCAGGS-BRG1 plasmid was transfected as shown in HepG2 cells, and pCAGGS empty load was used as a control, and the cell viability was detected with MTS (purchased from Promega) 48 hours after transfection. HepG2 cell viability was not affected.

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Abstract

The invention discloses an application of a chromatin remodeling complex core catalytic subunit to preparation of a drug for treating or preventing hepatitis B virus infection, and belongs to the field of antiviral drugs. A human BRG1 over-expression plasmid or small interfering RNA (ribonucleic acid) and a plasmid pHBV1.3 capable of expressing an HBV (hepatitis B virus) are co-transfected into ahepatic cell system HepG2 or Huh7, and the expression quantity of HBV secreted antigen HBeAg and HBsAg in cell culture supernate and the level of intracellular HBV replicative intermediate nucleocapsid related DNA (deoxyribonucleic acid) are detected to prove that BRG1 inhibits HBV replication and has hepatitis B virus resisting activity. As an antiviral mechanism is diversified, the virus cannoteasily generate drug resistance, and a safe and efficient polypeptide with small toxic and side effects is provided for clinical hepatitis B treatment.

Description

technical field [0001] The present invention relates to the field of antiviral drugs, and relates to a new application of a chromatin remodeling complex core catalytic subunit protein BRG1, and more specifically relates to a chromatin remodeling complex core catalytic subunit protein BRG1 in the preparation of treatment or prevention of B The application of hepatitis virus (Hepatitis B virus, HBV) infection medicine. Background technique [0002] Hepatitis B virus (HBV) infection is one of the main factors causing liver cancer, which seriously threatens human life and health. my country is a high-incidence area of ​​hepatitis B virus infection. There are about 120 million chronic hepatitis B patients, and about 300,000 people die of hepatocellular carcinoma caused by chronic hepatitis B every year. HBV belongs to the hepadnaviridae family, and its genome is partially double-stranded circular DNA. The core particle diameter of HBV is 28nm, which is a 20-hedron, and the vira...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61P31/20
CPCA61K38/1709A61P31/20
Inventor 刘兴晖宋惠徐莉敏徐凤霞沈振华刘倩倩鲁作华
Owner SHANGHAI CITY PUDONG NEW AREA GONGLI HOSPITAL
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