Metal-organic frameworks type nanometer drug and preparation method and application thereof

Abstract

The invention provides an MOFs type nanometer drug and a preparation method thereof in order to improve the deficiencies in an existing nanometer drug preparation technology. ZIF-8 is adopted as a drug delivery carrier, glucose oxidase (GOx) is encapsulated into channels of the ZIF-8, and MnO2 nanometer particles are supported to prepare the GM-ZIF-8 nanometer composite drug. The method for preparing the GM-ZIF-8 nanometer composite drug is simple in operation and mild in reaction condition, and no complicated preparation instruments are needed. The nanometer drug has a good inhibition effecton tumor cell proliferation.

Description

technical field

[0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a loaded metal-organic framework nanomedicine and a preparation method and application thereof. Background technique

[0002] Metal-organic frameworks (MOFs) are a class of crystalline hybrid materials composed of metal ions or metal cluster nodes coordinated with organic ligands. MOFs materials have a high specific surface area and porosity, which is beneficial to the loading and transportation of biological agents; MOFs materials can not only be used as a transport carrier for therapeutic drugs, but because of the diversity and controllability of their surfaces, it is reasonable to design and modify them , the intervention of corresponding components can realize the function of integration of diagnosis and treatment, and has great potential in accurate diagnosis and optimization of tumor treatment effect.

[0003] The infinite proliferation of tumor cells nee...

Examples

Embodiment 1

[0033] Preparation of GM / ZIF-8 Nanomedicine

[0034] experiment procedure:

[0035] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0036] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0037] 3) First add 2.5mg GOx to solution A, then add solution B to react for 30min under room temperature stirring, centrifuge, redissolve in 10mL pure water, add 500μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0038] 4) Examine the structure of GM / ZIF-8 with SEM and TEM.

[0039] test results:

[0040] See attached figure 1 , attached figure 2 . From the transmission electron microscope ...

Embodiment 2

[0043] Preparation of GM / ZIF-8 Nanomedicine

[0044] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0045] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0046] 3) First add 1mg GOx to solution A, then add solution B to react for 30min under room temperature stirring, centrifuge, redissolve in 10mL pure water, add 200μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0047] Test results: see Figure 4

Embodiment 3

[0049] Preparation of GM / ZIF-8 Nanomedicine

[0050] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0051] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0052] 3) First add 5 mg GOx to solution A, then add solution B to react for 30 min under room temperature stirring, centrifuge, redissolve in 10 mL of pure water, add 50 μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0053] Test results: see Figure 5

technical field

[0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a loaded metal-organic framework nanomedicine and a preparation method and application thereof. Background technique

[0002] Metal-organic frameworks (MOFs) are a class of crystalline hybrid materials composed of metal ions or metal cluster nodes coordinated with organic ligands. MOFs materials have a high specific surface area and porosity, which is beneficial to the loading and transportation of biological agents; MOFs materials can not only be used as a transport carrier for therapeutic drugs, but because of the diversity and controllability of their surfaces, it is reasonable to design and modify them , the intervention of corresponding components can realize the function of integration of diagnosis and treatment, and has great potential in accurate diagnosis and optimization of tumor treatment effect.

[0003] The infinite proliferation of tumor cells nee...

Examples

Embodiment 1

[0033] Preparation of GM / ZIF-8 Nanomedicine

[0034] experiment procedure:

[0035] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0036] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0037] 3) First add 2.5mg GOx to solution A, then add solution B to react for 30min under room temperature stirring, centrifuge, redissolve in 10mL pure water, add 500μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0038] 4) Examine the structure of GM / ZIF-8 with SEM and TEM.

[0039] test results:

[0040] See attached figure 1 , attached figure 2 . From the transmission electron microscope ...

Embodiment 2

[0043] Preparation of GM / ZIF-8 Nanomedicine

[0044] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0045] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0046] 3) First add 1mg GOx to solution A, then add solution B to react for 30min under room temperature stirring, centrifuge, redissolve in 10mL pure water, add 200μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0047] Test results: see Figure 4

Embodiment 3

[0049] Preparation of GM / ZIF-8 Nanomedicine

[0050] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0051] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0052] 3) First add 5 mg GOx to solution A, then add solution B to react for 30 min under room temperature stirring, centrifuge, redissolve in 10 mL of pure water, add 50 μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0053] Test results: see Figure 5

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