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Metal-organic frameworks type nanometer drug and preparation method and application thereof

A metal-organic framework and nano-drug technology, applied in the field of nano-materials, can solve the problems of immunogenic toxicity, short half-life, poor stability, etc., and achieve the effects of increased porosity, simple operation and mild reaction conditions

Active Publication Date: 2019-08-23
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, GOx itself has poor stability, short half-life in vivo, and has immunogenicity and systemic toxicity.
After exogenous injection of GOx, because of the ubiquitous presence of glucose and oxygen throughout the body, it leads to the uncontrollable production of H 2 o 2 causing systemic toxicity

Method used

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  • Metal-organic frameworks type nanometer drug and preparation method and application thereof
  • Metal-organic frameworks type nanometer drug and preparation method and application thereof
  • Metal-organic frameworks type nanometer drug and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of GM / ZIF-8 Nanomedicine

[0034] experiment procedure:

[0035] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0036] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0037] 3) First add 2.5mg GOx to solution A, then add solution B to react for 30min under room temperature stirring, centrifuge, redissolve in 10mL pure water, add 500μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0038] 4) Examine the structure of GM / ZIF-8 with SEM and TEM.

[0039] test results:

[0040] See attached figure 1 , attached figure 2 . From the transmission electron microscope ...

Embodiment 2

[0043] Preparation of GM / ZIF-8 Nanomedicine

[0044] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0045] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0046] 3) First add 1mg GOx to solution A, then add solution B to react for 30min under room temperature stirring, centrifuge, redissolve in 10mL pure water, add 200μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0047] Test results: see Figure 4

Embodiment 3

[0049] Preparation of GM / ZIF-8 Nanomedicine

[0050] 1) Dissolve 250mg BSA in 7mL pure water, add 3mL KMnO dropwise to the BSA solution at 37°C under continuous stirring 4 solution (0.067mmol / mL), dialyzed after 2h of reaction, constant volume to obtain MnO 2 Nanocolloids.

[0051] 2) 1.1g 2-MIM was dissolved in 5mL pure water to obtain solution A, and 0.1g (Zn(NO 3 ) 2 ·6H 2 O) was dissolved in 1 mL of pure water to obtain solution B.

[0052] 3) First add 5 mg GOx to solution A, then add solution B to react for 30 min under room temperature stirring, centrifuge, redissolve in 10 mL of pure water, add 50 μL MnO 2 The nanocolloid was stirred for 2 hours, centrifuged, washed twice with pure water, and dried under vacuum at room temperature to obtain GM / ZIF-8.

[0053] Test results: see Figure 5

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Abstract

The invention provides an MOFs type nanometer drug and a preparation method thereof in order to improve the deficiencies in an existing nanometer drug preparation technology. ZIF-8 is adopted as a drug delivery carrier, glucose oxidase (GOx) is encapsulated into channels of the ZIF-8, and MnO2 nanometer particles are supported to prepare the GM-ZIF-8 nanometer composite drug. The method for preparing the GM-ZIF-8 nanometer composite drug is simple in operation and mild in reaction condition, and no complicated preparation instruments are needed. The nanometer drug has a good inhibition effecton tumor cell proliferation.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials, and in particular relates to a loaded metal-organic framework nanomedicine and a preparation method and application thereof. Background technique [0002] Metal-organic frameworks (MOFs) are a class of crystalline hybrid materials composed of metal ions or metal cluster nodes coordinated with organic ligands. MOFs materials have a high specific surface area and porosity, which is beneficial to the loading and transportation of biological agents; MOFs materials can not only be used as a transport carrier for therapeutic drugs, but because of the diversity and controllability of their surfaces, it is reasonable to design and modify them , the intervention of corresponding components can realize the function of integration of diagnosis and treatment, and has great potential in accurate diagnosis and optimization of tumor treatment effect. [0003] The infinite proliferation of tumor cells nee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/54A61K47/52A61K38/44A61K33/32A61P35/00C01G45/02B82Y40/00B82Y30/00
CPCA61K47/54A61K47/52A61K38/443A61K33/32A61P35/00C01G45/02B82Y40/00B82Y30/00A61K2300/00
Inventor 田甘丁帅帅曾丽娟卞修武
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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