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Propargite hapten and synthesis method and application thereof

A synthesis method and technology of propargite, which are applied in the field of hapten and synthesis of propargite, achieve the effects of simple experimental operation, high purity and yield, and cheap and easy-to-obtain raw materials

Pending Publication Date: 2019-08-23
SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no relevant report on the hapten of propargite in China

Method used

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  • Propargite hapten and synthesis method and application thereof
  • Propargite hapten and synthesis method and application thereof
  • Propargite hapten and synthesis method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing a propargite hapten, comprising the steps of:

[0039] (1) 4-tert-butylphenol and 1,2-epoxy-4-vinylcyclohexane react to prepare intermediate product I:

[0040] Add 4-tert-butylphenol (1.00g, 6.66mmol) and 1,2-epoxy-4-vinylcyclohexane (1.00g, 8.00mmol) successively to a 50ml single-necked bottle, stir at room temperature until dissolved; Add potassium hydroxide (75mg, 1.33mmol) and toluene (1.00ml), raise the temperature to 105°C and stir for 18h; drop to room temperature and stir, add toluene (20ml), wash with 5% sodium hydroxide solution (60ml) in three equal portions, Get the organic layer; the organic layer is dried with anhydrous sodium sulfate, filtered, and the filtrate is taken; the filtrate column chromatography, the eluent is petroleum ether: ethyl acetate, and the volume ratio is 10:1; the target liquid is separated, and the solvent is removed by distillation. 1.7 g of a colorless transparent oily substance was obtained, which was the ...

Embodiment 2

[0048] A method for preparing a propargite hapten, comprising the steps of:

[0049] (1) 4-tert-butylphenol and 1,2-epoxy-4-vinylcyclohexane react to prepare intermediate product I:

[0050] Add sodium hydroxide (350mg, 8.66mmol) and purified water (13.00ml) to a 50ml single-necked bottle in turn, stir at room temperature until it dissolves; add 4-tert-butylphenol (1.00g, 6.66mmol), stir at room temperature until it dissolves Add 1,2-epoxy-4-vinylcyclohexane (1.00g, 8.00mmol) dropwise in 3 to 5 minutes, stir at room temperature until dissolved, and react at room temperature for 18 hours; filter the reaction solution, and filter the cake with purified water ( 40ml) was washed twice, and then washed with n-hexane (20ml), and the obtained filter cake was dried at room temperature; 1.65g of white solid was intermediate product I, yield: 90.3%.

[0051] (2) intermediate product I reacts with sulfur oxychloride to prepare intermediate product II

[0052] Add toluene (1.10ml) and t...

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Abstract

The invention aims to provide a propargite hapten and a synthesis method and application thereof. By combining structural characteristics of propargite to design the synthesis method of the propargitehapten and applying the synthesis method of the propargite hapten to synthesis of an artificial propargite antigen, an immune animal is induced to generate an antibody, and various immunoassay methods of propargite are built. The synthesis method of the propargite hapten comprises the following steps of (1) carrying out reaction on p-tert-butylphenol and 1,2-epoxy-4-vinylcyclohexane to produce anintermediate product I; (2) carrying out reaction on the intermediate product I and thionyl chloride to produce an intermediate II; (3) carrying out reaction on the intermediate II and propargyl alcohol to produce an intermediate III; and (4) carrying out reaction on the intermediate III and 3-mercaptopropionic acid to produce the hapten IV. According to the synthesized propargite hapten, not only is the characteristic structure of the propargite maintained furthest to obviously enhance the immunogenicity of the propargite hapten, but also the synthesized propargite hapten has a carboxyl group which can be coupled with a carrier and provides a basis for building the various immunoassay methods of the propargite subsequently.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a propargite hapten and its synthesis method and application. Background technique [0002] Acetate, also known as gramate, is an organic sulfur acaricide with high efficiency, low toxicity, broad-spectrum and long-lasting effect. It can effectively prevent and control the harm of mites. Control various pest mites in cotton, vegetables, apples, citrus, tea, flowers and other crops. The invention has the advantages of good control effect, low cost, not easy to produce drug resistance and the like. It has low toxicity to humans and animals, and is safe to natural enemies and economic insects such as ladybugs, lacewings, bees and silkworms. It is very popular because of its remarkable medicinal effect and it is not easy to produce drug resistance. A small amount of pesticide residues will not cause acute poisoning, but long-term consumption of fruits and...

Claims

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Application Information

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IPC IPC(8): C07C323/64C07C319/16C07C301/00C07C41/03C07C43/205G01N33/68
CPCC07C41/03C07C301/00C07C319/16C07C323/64C07C2601/14G01N33/68C07C43/205
Inventor 叶雅真田宏杨星星魏雄军黎维杨中杨林林
Owner SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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