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Microfluidic device for screening high motility sperm

A microfluidic device, high activity technology, applied in enzymology/microbiology devices, biochemical cleaning devices, biochemical instruments, etc., can solve the problems of unavoidable centrifugal operation, inability to guarantee sperm activity, sperm damage, etc., to achieve structural The effect of compact and reasonable setting, convenient application and low production cost

Inactive Publication Date: 2019-08-27
NANJING JINGJING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a microfluidic device for high-motility sperm screening, which solves the problem of unavoidable centrifugal operation, density gradient centrifugation and separation liquid in the existing sperm screening process. Influence of ingredients The operation will cause damage to the sperm, and the problem of sperm activity cannot be guaranteed

Method used

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  • Microfluidic device for screening high motility sperm
  • Microfluidic device for screening high motility sperm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A microfluidic device for screening high-motility sperm, comprising a cylinder, the inner cavity of the cylinder is divided into two inner and outer chambers by a thin annular wall, and the inner chamber and the outer chamber are concentric circular structures, and the inner chamber The chamber is filled with sperm nutrient solution, the outer chamber is equipped with a semen sample, and the semen sample just covers the upper surface of the inner chamber, the radius of the inner chamber is 2.5 mm, and the depth is 20 mm. The ring-shaped net radius of the outer chamber is 2mm, the depth is 20mm, the thickness of the thin annular wall between the inner and outer chambers is 0.5mm, and the height is 20mm. The sperm nutrient solution is a self-prepared sperm nutrient solution, and the semen sample comes from a male patient of an infertile couple. The sperm nutrition In the process of self-preparation, the solution is composed of the following components: NaCl 0.721g, MgSO 4...

Embodiment 2

[0054] A microfluidic device for screening high-motility sperm, comprising a cylinder, the inner cavity of the cylinder is divided into two inner and outer chambers by a thin annular wall, and the inner chamber and the outer chamber are concentric circular structures, and the inner chamber The chamber is filled with sperm nutrient solution, the outer chamber is equipped with a semen sample, and the semen sample just covers the upper surface of the inner chamber, the radius of the inner chamber is 2.5 mm, and the depth is 20 mm. The ring-shaped net radius of the outer chamber is 2mm, depth 20mm, thickness of the thin annular wall between the inner and outer chambers is 0.5mm, height 20mm, sperm nutrient solution is commercial Earle's solution, and semen samples are from male patients of infertile couples.

[0055] A method for preparing a high-motility sperm screening microfluidic device, specifically comprising the following steps:

[0056] S1. Prepare a nickel-based, monocrys...

Embodiment 3

[0070] A microfluidic device for screening high-motility sperm, comprising a cylinder, the inner cavity of the cylinder is divided into two inner and outer chambers by a thin annular wall, and the inner chamber and the outer chamber are concentric circular structures, and the inner chamber The chamber is filled with sperm nutrient solution, the outer chamber is equipped with a semen sample, and the semen sample just covers the upper surface of the inner chamber, the radius of the inner chamber is 2.5 mm, and the depth is 20 mm. The ring-shaped net radius of the outer chamber is 2mm, depth 20mm, thickness of the thin annular wall between the inner and outer chambers is 0.5mm, height 20mm, sperm nutrient solution is BWW solution, and semen samples are from male patients of infertile couples.

[0071] A method for preparing a high-motility sperm screening microfluidic device, specifically comprising the following steps:

[0072] S1. Prepare a nickel-based, monocrystalline silicon...

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Abstract

The invention discloses a microfluidic device for screening high motility sperm, and relates to the technical field of biology. The device comprises a cylinder body, wherein the inner cavity of the cylinder body is divided into an inner chamber and an outer chamber by a thin layer annular wall; the inner and outer chambers are of concentric circle structures; the inner chamber is filled with a sperm nutrient solution; the outer chamber is filled with a sperm sample; the sperm sample just covers the upper surface of the inner chamber. According to the microfluidic device for screening the highmotility sperm, sperm characteristics of swim-up and swimming motility along an interface are utilized, potential adverse effects of various operations of in vitro centrifugation, washing and the likeon the sperm in an existing assisted reproductive technology process are avoided, structure arrangement is compact and reasonable, the high motility sperm can be effectively concentrated, the high motility sperm needed by an assisted reproductive technology can be obtained so as to greatly improve success rate of in vitro fertilization, the application range is wide, the device is not only applicable to human reproduction such as reproductive centers of various medical institutions, but also applicable to animal breeding, and the device can be used in both human reproduction and animal breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microfluidic device for screening high-motility sperm. Background technique [0002] The incidence of infertility in the world is about 15%-20%, and there is an increasing trend year by year. According to WHO prediction, in the near future, reproductive health problems will be the next threat to human health after cardiovascular and cerebrovascular diseases and tumors. The third major disease in China, the increase in the incidence of infertility, has greatly promoted the development of assisted reproductive technology. Many infertile couples have brought good news; the second-generation IVF technology - intracytoplasmic sperm injection (ICSI) technology and the third-generation technology - preimplantation genetic diagnosis (PGD) have been further enriched and improved However, many operations in the process of assisted reproductive technology may cause damage to sperm, eventuall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00B01L3/00
CPCB01L3/502707C12M47/04
Inventor 陆金春
Owner NANJING JINGJING BIOTECHNOLOGY CO LTD