Neuroprotection effect of biochanin A

A technology for chickpea sprouts and neuroprotection, which can be applied in the fields of nervous system diseases, medical preparations containing active ingredients, peptide/protein ingredients, etc., and can solve problems such as little research.

Inactive Publication Date: 2019-08-30
TAISHAN MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

In recent years, the interaction between GH and estrogen has been confirmed by many experiments, but there are few studies on the influence of the two on the nervous system

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0006] The present invention will be described in detail below in combination with specific embodiments.

[0007] The neuroprotective effect of biochanin A, specifically, biochanin A plays a neuroprotective role through the regulation mechanism of growth hormone JAK / STAT signaling pathway.

[0008] The hippocampal neurons were stimulated with different concentrations of GH (5, 25, 125, 250, 500 ng / ml) for 24 hours, and the effect of GH on the viability of hippocampal neurons was observed by CCK8 method. According to the screened optimal concentration of BioA and GH, after 24 hours of acting alone or in combination on hippocampal neurons, CCK8 was used to measure the effect of single or combined drugs on the viability of hippocampal neurons, and to observe whether the combination of the two drugs had a synergistic effect. Choose different concentrations of GH (0.1, 1, 5, 10, 20ng / ml) or different action time (15, 30, 60, 90min) to stimulate the hippocampal neuron cells, extract...

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Abstract

The invention discloses a neuroprotection effect of biochanin A. The biochanin A achieves the neuroprotection effect through the regulatory mechanism of a growth hormone JAK/STAT signal path. The 125ng/ml of GH and 10<-5>mol/L of Bio A are used for stimulating a hippocampal neuron independently or jointly, combined medicine has a better effect for promoting cell reproduction of hippocampal neuronsthan that of a single medicine, when the Bio A at 10<-8>mol/L acts for 60 minutes, the cell SOCS2 protein has the most remarkable expression difference. Combined administration of the Bio A and GH can remarkably increase the expression level of STAT5 and P-STAT5 proteins, and further, administration of treating the Bio A 60 minutes earlier than treating the GH can cause up-regulation of the expression level of the STAT5 and P-STAT5 proteins more than simultaneous administration of the Bio A and GH; and the expression level of SOCS2 protein can be down regulated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the neuroprotective effect of chickpein A by regulating the growth hormone JAK-STAT signal pathway. Background technique [0002] Alzheimer's disease (AD), commonly known as senile dementia, belongs to the categories of "dementia", "dementia" and "dementia" in traditional Chinese medicine, and is one of the most common types of senile dementia. Epidemiological and clinical experimental studies at home and abroad have shown that with the decline of estrogen levels in middle-aged and elderly women after menopause, their risk and rate of AD will also increase greatly, and the degree of cognitive impairment in AD patients is significantly higher than that in elderly patients. male. For example, giving estrogen replacement therapy (hormone replacement therapy, HRT) to perimenopausal women (especially women after oophorectomy) can greatly reduce the risk of AD and improve the cognitive func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K38/27A61P25/28
CPCA61K31/352A61K38/27A61P25/28
Inventor 周延萌王凯楠
Owner TAISHAN MEDICAL UNIV
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