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Lichen sclerosus type vulvar dystrophy biologic marker, screening method and detection reagent kit

A vulvar dystrophy and biomarker technology, applied in the field of biomedicine, can solve the problem of a large age span of patients

Inactive Publication Date: 2019-08-30
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the age span of patients with this disease is very large, and it is difficult to use hormones as a factor to explain the clinical characteristics of this disease

Method used

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  • Lichen sclerosus type vulvar dystrophy biologic marker, screening method and detection reagent kit
  • Lichen sclerosus type vulvar dystrophy biologic marker, screening method and detection reagent kit
  • Lichen sclerosus type vulvar dystrophy biologic marker, screening method and detection reagent kit

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Sample collection

[0033] Criteria for sample inclusion: women of childbearing age, high hormone expression level, uterus, no cervical erosion, no HPV infection.

[0034] Number of samples collected: 40 cases of healthy people, 40 cases of vulvar lichen sclerosus.

[0035] The vaginal secretions of the volunteers were collected, and the volunteers had no record of vaginal medication 3 days before the sampling.

[0036] 2. DNA extraction

[0037] Collection and processing of vaginal secretion samples: Put the collected vaginal secretion samples into sterile 1.5ml EP tubes, immediately put them on ice to freeze the samples, then send the frozen samples to the storage point, and add 50 μl of ethanol, stored at -80°C until sequencing analysis.

[0038] DNA extraction was carried out according to the steps of the Omega kit.

[0039] 3. Sequencing library construction

[0040] The sample DNA was amplified by PCR using bacterial 16S rRNA-specific V3-V4 region amplific...

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Abstract

The invention provides a lichen sclerosus type vulvar dystrophy biologic marker, a screening method and a detection reagent kit. The biologic marker comprises Streptococcus and / or Coprocossus. The biologic marker provided by the invention can simply and effectively detect whether objects suffer from early lichen sclerosus type vulvar dystrophy symptoms or not; and besides, through detecting the changes of the level of the biologic marker after the objects are in contact with a candidate medicine, whether the candidate medicine can be used as a medicine for treating or preventing the lichen sclerosus type vulvar dystrophy or not can be determined.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a biomarker of lichen sclerosus vulvar dystrophy, a screening method and a detection kit. Background technique [0002] Vulvar dystrophy is a group of chronic diseases in which the skin and mucous membranes of female vulva undergo degeneration and pigmentation changes. Vulvar dystrophy can be divided into vulvar lichen sclerosus type and vulvar squamous cell simple hyperplasia type according to its pathological types. The typical clinical manifestations are unbearable vulvar itching or / and pain, rough vulva skin, lichen-like thickening, local hypopigmentation, and even chapped and atrophied. Sexual life seriously affects the patient's quality of life. In recent years, there is still a clear upward trend in the incidence, and at the same time, the younger age is more obvious. [0003] The cause of vulvar dystrophy is currently unknown, and the pathogenesis is unc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883
Inventor 牛晓宇陈勉之
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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