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Production process for producing tricholoma matsutake mycelium from corn powder fermentation

A production process, corn flour technology, applied in horticulture, application, botanical equipment and methods, etc., to achieve the effects of low cost, increased dry weight of mycelium, and short production cycle

Inactive Publication Date: 2019-09-06
LINYI XINBANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a production process for producing matsutake mycelium by fermenting corn flour, which has the advantage of high dry matter yield of matsutake mycelium, and solves the problem of matsutake mycelium in the prior art. The dry matter yield of mycelium is only about 18g / L, and there is still a lot of room for increasing the dry matter yield of mycelium per unit volume of culture medium

Method used

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  • Production process for producing tricholoma matsutake mycelium from corn powder fermentation
  • Production process for producing tricholoma matsutake mycelium from corn powder fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] see figure 1 , a production process utilizing corn flour fermentation to produce matsutake mycelium, comprising the following steps:

[0035] 1. Strain cultivation on slant: Take matsutake sporocarp that has not broken the bacterium film, and after repeated surface sterilization with 70% ethanol by mass, remove a small piece with sterile tweezers and a knife, and insert it into the slant medium Seal it well, put it in an incubator, and cultivate it under a constant temperature condition of 25°C. After the mycelium grows all over the slope, it will be transferred to the next step of cultivation;

[0036] 2. Liquid seed culture: insert the slant bacterial classification cultivated in step 1 into the corn flour liquid seed medium in a shaker bottle, cultivate it on a shaker at 25° C. for 8 days at 190 r / min, filter aseptically to obtain mycelium, and use Rinse with sterile water, add four times the volume of normal saline and a small amount of glass beads to dilute, smash...

experiment example 1

[0049] Experimental example one: according to the mycelia fermentation method in the embodiment one of this program and the mycelia fermentation method in the prior art, get the matsutake mycelia of one week and carry out mycelium dry weight experiment contrast, obtain figure 2 middle data;

[0050] Depend on figure 2 It can be seen that after 1 day of fermentation in this scheme, the dry weight of mycelia in this scheme tends to increase in the first four days, and the dry weight of mycelium in this scheme reaches the highest value on the 4th day, and the dry weight of mycelia at this time is 24.88g / L, after the 6th day of fermentation, the dry weight of the mycelium began to decrease significantly. From this, it can be seen that matsutake bacteria began to grow in several phases on the second day of fermentation, and all indicators increased rapidly, and the metabolism was very active. After reaching the maximum value, matsutake bacteria The growth and metabolism are rel...

experiment example 2

[0053] Experimental example 2, take the same size about 2cm 2 5 pieces of bacterium block, respectively numbered as A, B, C, D, E, cultivate respectively by the method for embodiment one, and get the mycelia of the 4th day and carry out dry matter weight measurement, the result is as follows:

[0054] Experimental example A B C D E Mycelium dry matter yield (g / L) 25.03 24.82 24.95 24.91 24.97

[0055] It can be seen from the above table that the highest yield of mycelium dry matter fermentation produced by this process is above 24.82g / L, while the dry matter yield of mycelium in the prior art is only about 18g / L, so compared with the existing The production process of mycelium dry matter of the technology has been significantly improved. It can be seen that the present invention is suitable for the fermentation and cultivation of matsutake fungus. The yield of mycelium dry matter is stable and the output is high, and it can be produced on a large s...

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Abstract

The invention discloses a production process for producing tricholoma matsutake mycelium from corn powder fermentation and belongs to the field of production of tricholoma matsutake mycelium. The production process includes the steps of 1, culture of inclined bacterial; 2, culture of liquid seeds; 3, fermentation culture; 4, dry weight of the mycelium. The experimental results show that the highest yield of dry matter fermentation of the mycelium produced by the process is above 24.82g / L while the dry matter yield of the mycelium is only about 18g / L in the existing technology, so that comparedwith the prior art, the production process for producing the tricholoma matsutake mycelium has the advantage of significant improvement; the production process is suitable for fermentation and culture of the tricholoma matsutake, the dry material yield of the mycelium is stable and high in yield, large-scale production can be achieved, and the production process is worth popularizing.

Description

technical field [0001] The invention relates to the field of matsutake mycelium production, in particular to a production process for producing matsutake mycelium by fermenting corn flour. Background technique [0002] Matsutake mushroom, also known as matsutake mushroom, is a precious fungus. It is delicious, nutritious, and has a high medicinal and therapeutic effect. Matsutake mushrooms are born in pine forests or mixed coniferous and broad-leaved forests in autumn. They are distributed in Northeast China and Southwest my country, and also in Japan and Korea abroad. Matsutake cannot be cultivated artificially. It is a rare and precious natural edible fungus. [0003] Due to the high nutritional value and medicinal value of matsutake mycelium, the annual output is decreasing year by year, so it is necessary to strengthen the research on matsutake conservation and promotion technology, especially the breeding of matsutake mycelium obtained by liquid fermentation in odor an...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/20A01G18/40
Inventor 陈勇勇张彦华袁中信刘德朋安桂云
Owner LINYI XINBANG BIOTECH CO LTD
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