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Method for inducing embryogenic callus of coix lacryma-jobi

A technology of embryogenic callus and callus, applied in the field of induction of embryogenic callus, can solve the problems of backward research depth, domestication degree of cultivated varieties, high quality and high yield and other economic traits that have not been studied in depth, and have reached a huge Use prospects, accelerated research and application effects

Inactive Publication Date: 2019-09-06
安徽省农业科学院棉花研究所
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Problems solved by technology

At present, the technical level and research depth of coix planting lag behind other gramineous crops in an all-round way, especially in terms of germplasm resources, and the domestication degree, high-quality and high-yield economic traits of cultivated and applied varieties have not been studied in depth

Method used

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Embodiment Construction

[0014] The technical scheme of the present invention will be further described below in conjunction with specific examples:

[0015] A method for inducing embryogenic callus of Job's tears, comprising the following steps:

[0016] 1. Aseptic embryo culture

[0017] Coix seeds from which the involucre was removed were soaked in 75% alcohol for 2 minutes, rinsed with sterile water for 3 times, and cultured on a sterile water paper bed. After germination, sterilize with 0.1% mercury liter for 8 minutes, rinse with sterile water for 5 to 8 times, cut off the germ, and inoculate it in N 6 The culture medium is sterile cultured, the temperature is (22±1)°C, and the light intensity is 2000lx.

[0018] 2. Callus induction

[0019] After the buds are 3cm long, slice them obliquely and transfer them to the culture medium (N 6 +2.0mg / L 2,4-D+500mg / L proline+300mg / L hydrolyzed casein+1.0mg / L AgNO 3 +30g / L sucrose), pH 5.8, temperature (22±1)°C, light for 13h, 1200lx, subculture 2 to ...

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Abstract

The invention discloses a method for inducing an embryogenic callus of coix lacryma-jobi. The method comprises the following steps: (1) sterile germ culture; (2) callus induction; (3) callus proliferation culture; and (4) embryogenic callus transformation culture. A scientific method for inducing the embryogenic callus of the coix lacryma-jobi is established, and the rapid operation cycle can meetthe establishment and research of high-quality coix lacryma-jobi germplasm resources, a remarkable promotion effect on accelerating the research and application of the coix lacryma-jobi resources isachieved, and the application prospect is huge.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for inducing embryogenic callus of Job's tears. Background technique [0002] The induction of embryogenic callus is the key to achieve morphogenesis and redifferentiation, and the induction of compact embryogenic callus to form new plants is an important way to establish most plant regeneration systems. At present, Coix planting lags behind other gramineous crops in terms of production and application technology and research depth, especially in terms of germplasm resources, and the domestication degree, high-quality and high-yield economic traits of cultivated and applied varieties have not been studied in depth. The application of modern cell engineering technology can quickly improve resources through genetic transformation, and accelerate the research and application of Coix resources. Contents of the invention [0003] The object of the pre...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 江本利闫晓明程福如朱加保於春路献勇王红娟胡积送
Owner 安徽省农业科学院棉花研究所
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