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Marine crustacean water ecological toxicity test method

A technology of toxicity test and water ecology, which is applied in the direction of testing water, material inspection products, measuring devices, etc.

Active Publication Date: 2019-09-06
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Algae, crustaceans and fish toxicity test methods exist in the prior art, but the selected species to be tested are freshwater species, and there is no aquatic ecotoxicity test method applicable to saline conditions in China at present, and there is no test for biological toxicity test Most of the organisms choose freshwater fish, shellfish, etc. These methods are only applicable to the freshwater field, and the selection of suitable test organisms for seawater toxicity tests has become a research focus

Method used

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  • Marine crustacean water ecological toxicity test method
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  • Marine crustacean water ecological toxicity test method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] At different naphthalene concentrations: 0 μg / L, 20 μg / L, 33.1 μg / L, 54.8 μg / L, 90.6 μg / L, 150 μg / L, the changes of pH value, dissolved oxygen and temperature during the mysis acute toxicity test are shown in the table As shown in 1, the mortality rate of mysis after 96h is as follows figure 1 shown.

[0040] Table 1: Water quality parameters of naphthalene acute toxicity test on mysis

[0041]

Embodiment 2

[0043] In different sodium sulfate concentrations: 0g / L, 3g / L, 3.95g / L, 5.20g / L, 6.84g / L, 9g / L, the changes of pH value, dissolved oxygen and temperature during the acute toxicity test of mysid shrimp are as follows: As shown in table 2, the mortality rate of mysis after 96h is as follows figure 2 shown.

[0044] Table 2: Water quality parameters of sodium sulfate acute toxicity test on mysis

[0045]

[0046]

[0047] According to the calculation of the mysis acute toxicity test data of embodiment 1 and 2, it can be obtained: the 96h LC of naphthalene to mysis 50 (Half lethal concentration) is 65.27μg / L, and its 95% confidence interval range is 51.42~82.85μg / L; The 96h LC of sodium sulfate to mysis 50 It was 5.73g / L, and its 95% confidence interval ranged from 5.02 to 6.54g / L. By measuring the pH value, dissolved oxygen and temperature in the test, the test data are shown in Table 1 and Table 2. The pH value, dissolved oxygen and temperature fluctuate slightly within ...

Embodiment 3

[0049] After the test was carried out for 7 days, the survival number and average Changes in body weight, average body length, number of females, and average reproduction number are shown in Table 3-Table 6.

[0050] Table 3: Survival numbers of Mysis shrimp chronic toxicity test

[0051]

[0052] Table 4: Average body weight and body length of mysis chronic toxicity test

[0053]

[0054]

[0055] Table 5: Number of females in mysis chronic toxicity test

[0056] Concentration (μg / L) mean ± standard deviation Significant differences control group 2.00±1.41 none 5 2.50±1.29 P=0.97>0.05, the difference is not significant 10 2.00±0.82 P=1>0.05, the difference is not significant 20 2.25±1.26 P=0.999>0.05, the difference is not significant 50 2.75±1.71 P=0.871>0.05, the difference is not significant 70 2.25±0.96 P=0.999>0.05, the difference is not significant

[0057] Table 6: Average reproduction number of My...

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Abstract

The present invention discloses a marine crustacean water ecological toxicity test method. The method comprises: selecting the mysis stage as a tested crustacean, and taming the mysis stage in standard water with the same water quality as a tested water sample; preparing to-be-tested water sample reserve liquid; by performing acute toxicity and chronic toxicity pre-tests of the mysis stage, respectively selecting a concentration gradient range, and setting the concentration gradient with a small gap; taking the seawater as a comparison group, and the to-be-tested water sample as a test group,and simultaneously performing acute toxicity and chronic toxicity tests of the mysis stage, recording the change of the mysis stage and the environment, performing variance analysis on the data, and drawing a mortality curve of the mysis stage; and in combination of the signs of the mysis stage and the change of water indicators, determining the water toxicity. According to the method provided bythe present invention, the defect that there is no water ecological toxicity test method applicable to salty conditions in the prior art is overcome, and formulating national standards for marine sewage discharge can be facilitated.

Description

technical field [0001] The invention relates to a marine crustacean water ecotoxicity test method, in particular to a method for detecting seawater quality toxicity through acute toxicity and chronic toxicity tests. Background technique [0002] Marine wastewater and effluent discharges, such as oil leakage, discharge of ship exhaust desulfurization waste liquid, discharge of ship ballast water, discharge of domestic sewage, etc., contain a large amount of toxic and harmful substances, such as heavy metals and polycyclic aromatic hydrocarbons in the washing water of ship exhaust desulfurization, etc. Disinfection by-products are produced during the treatment of ship ballast water. There are various types of marine pollutants, strong toxicity and wide spread, which will make it difficult to control marine pollution. After a long period of accumulation, the outbreak will cause great harm to the marine ecological environment. [0003] Biotoxicity test is one of the commonly us...

Claims

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Application Information

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IPC IPC(8): G01N33/18
CPCG01N33/186
Inventor 张拿慧凌云彭自然朱荧
Owner SHANGHAI OCEAN UNIV
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