A kind of gold silver nano flower particle with core-shell structure and its preparation method and application

A core-shell structure and nanoparticle technology, which is applied in the preparation of nanomaterials and biomedicine, can solve the problems of complex synthesis steps of gold nanoflower particles, limit the application of gold nanoflower particles, and cumbersome post-modification process, and achieve good photothermal conversion Efficiency, sensitive surface, effect of enhanced Raman activity

Active Publication Date: 2021-11-02
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the synthesis methods of gold nanoflower particles reported in the literature generally have problems such as complex synthesis steps, harsh synthesis conditions, cumbersome post-modification process, and the use of biotoxic surfactants as stabilizers, which limit the use of gold nanoflower particles. Further applications in biomedicine

Method used

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  • A kind of gold silver nano flower particle with core-shell structure and its preparation method and application
  • A kind of gold silver nano flower particle with core-shell structure and its preparation method and application
  • A kind of gold silver nano flower particle with core-shell structure and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of gold and silver nanoflower particles:

[0032] (1) Preparation of tea polyphenol-coated silver-core nanoparticles: Add 20 mg of tea polyphenol powder into a 150 mL beaker, add 15 mL of water to dissolve it, then add 176 μL of 20 mM silver nitrate solution, stir and mix evenly Finally, cover the beaker with a watch glass. As for the microwave oven, react at high temperature (100-150 ℃, 1000-1500 W) for 2 minutes. After the reaction, centrifuge at 8000 rpm for 10 minutes, and then wash with water for 3 minutes. Second, obtain silver core nanoparticles;

[0033] (2) Preparation of gold and silver nanoflower particles with a core-shell structure: Add 10 mL of the silver core nanoparticle solution obtained in step (1) into a round bottom flask, and add 400 μL of 17.6 mM chloroauric acid under rapid stirring The solution was reacted at room temperature for 5 minutes. After the reaction, it was centrifuged at 8000 rpm for 10 minutes, and then washe...

Embodiment 2

[0037] Example 2 Photothermal heating performance of gold and silver nanoflower particles

[0038] Prepare different concentrations (0-50 μg mL -1 ) of gold and silver nanoflower particle solution, placed in a quartz cuvette, the wavelength of the laser is adjusted to 808 nm, and the power is 2 watts / square centimeter. The solution was irradiated with laser light for 10 minutes. During the period, the temperature of the solution was recorded by a thermocouple probe inserted in it, and the results were as follows: Figure 4 shown. Depend on Figure 4 It can be seen that the gold and silver nanoflower particles can quickly absorb light energy and generate heat under laser irradiation, which makes the water heat up rapidly. Moreover, the greater the concentration of gold and silver nanoflower particles, the more significant the heating effect, which is expected to be used in photothermal therapy.

Embodiment 3

[0039] Example 3 Photothermal Cytotoxicity of Gold and Silver Nanoflower Particles

[0040] Preparation of gold and silver nanoflower particles modified with mercaptopolyethylene glycol 2000: Add mercaptopolyethylene glycol 2000 (purchased from Xi'an Ruixi Biotechnology Co., Ltd.) to 2 mL of 1 mg mL -1 In the gold and silver nanoflower particle solution, a mixed solution with a final concentration of mercapto polyethylene glycol 2000 of 20 mM was prepared, reacted for 12 hours, and the product was washed with water for 3 times, and finally stored at 4 °C.

[0041] The photothermal cytotoxicity of gold and silver nanoflower particles was evaluated by CCK-8 method. HeLa cells were first incubated in a 96-well plate for 24 hours, and different concentrations (0-50 μg mL -1) surface-modified gold and silver nanoflower particle solution, and then incubated for 6 hours. Afterwards, each well was irradiated with a laser for 5 minutes, and continued to incubate for 12 hours, and the...

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Abstract

The invention discloses a gold-silver nano-flower particle with a core-shell structure and its preparation method and application. It uses tea polyphenols as a reducing agent and a stabilizer to rapidly synthesize gold-silver nano-flower particles without using additional surfactants and Seed crystal, low energy and environmental protection. The surface of the obtained gold-silver nanoflower particles is distributed with dense multi-branched gold petals, which make it exhibit remarkable near-infrared light absorption properties and good photothermal heating performance, and can be applied to photothermal therapy based on near-infrared light; At the same time, the obtained gold and silver nanoflower particles also exhibit excellent stability and good surface-enhanced Raman performance, and are expected to be used for Raman imaging of cells, so their application prospects in cancer diagnosis and treatment are good.

Description

technical field [0001] The invention belongs to the fields of nano-material preparation and biomedicine, and in particular relates to a gold-silver nano-flower particle with a core-shell structure and a preparation method and application thereof. Background technique [0002] Cancer is a general term for a large class of malignant tumors, which has become a major global problem that endangers public health and hinders the development of the global economy. In recent years, the rise of nanomedicine has brought new opportunities for the development of new methods for efficient and targeted cancer treatment. Due to their unique optical, thermal, acoustic and magnetic properties, nanomaterials are widely used in photoimaging (such as photoacoustic imaging, optical imaging) and phototherapy (such as photothermal therapy, photodynamic therapy, photoinduced chemotherapy) A platform for the integration of disease diagnosis and treatment can be established, which can greatly improve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/00B22F9/24B82Y30/00B82Y40/00
CPCB22F9/24B82Y30/00B82Y40/00B22F1/0553B22F1/07B22F1/054
Inventor 郑琤李娟李婧影杨黄浩
Owner FUZHOU UNIV
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