High-throughput detecting system for obtaining trace mass and molecular structure information

Abstract

The invention discloses a high-throughput detecting system for obtaining trace mass and molecular structure information. A micro cantilever array fixed on a piezoelectric ceramic actuator is driven bya voice coil motor to translate along a width direction of a micro cantilever beam, so that laser beams are projected one by one onto the micro cantilever array, the laser reflected by a tip is detected by a photoelectric position sensitive detector to obtain a bending signal of a free end of each micro cantilever beam, and the vibration frequency of the micro cantilever beam is adjusted by the piezoelectric ceramic actuator; a micro Raman spectrum unit is disposed and is composed of an observation optical path sub-unit and a Raman laser optical path sub-unit, which work in a time division manner; the microstructure of a sample on the surface of the free end of the micro cantilever beam is obtained by using the observation optical path sub-unit; and a Raman spectrum is obtained by using the Raman laser optical path sub-unit, and then the molecular structure information of the sample is obtained. The high-throughput detecting system disclosed by the invention combines the micro cantilever beam sensing and micro Raman technology to realize the detection of trace mass changes, and is used for observing the appearance of the sample, and detecting the change of a molecular structure ina reaction process in real time.

Description

technical field [0001] The invention relates to the field of micro-cantilever beam detection and Raman spectrum analysis, and more specifically relates to a high-throughput detection system for obtaining trace mass and molecular structure information. Background technique [0002] Micro-cantilever sensing technology is a new sensing method developed rapidly after the appearance of atomic force microscope and microsystem. As the simplest micro-mechanical component, it has always been a hot spot in the research of micro-nano sensing technology. The microbeam sensor can measure the specific biochemical reaction process in real time. When the biochemical reaction occurs on the surface of the microbeam, the stress difference between the upper and lower surfaces of the microbeam causes it to bend and deform. As a real-time, high-sensitivity, non-calibration sensing method, this sensing technology has been widely studied in the fields of biology and chemistry. [0003] Generally, ...

AI Technical Summary

Problems solved by technology

[0006] In the existing technology, from the perspective of equipment, the continuous update and improvement of the system is aimed at detecting the change of trace mass of the micro-cantilever; from the perspective of detection mechanism, no matter how good the repeatability of the bending signal of the micro-cantilever is, it can only Obtaining a single bending response signal at the free end of the micro-cantilever beam cannot detect other information of the tested material in real time, such as structural information, so the mechanism cannot be analyzed from the perspective of structure and mechanical properties

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