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Method for quantitatively detecting main components of Shuangshen Pingfei granules in plasma at same time
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A quantitative detection and plasma technology, which can be used in the research of in vivo metabolism of Chinese patent medicines and the field of medicine, which can solve problems such as unreported
Active Publication Date: 2019-09-13
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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However, there is no report on the pharmacokinetics of these nine components in rat plasma after oral administration of Shuangshen Pingfei Granules
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Embodiment 1
[0076] Example 1: Establishment of a method for determining the content of main components in rat plasma after oral administration of Shuangshen Pingfei Granules
[0079] The rats were fed adaptively for one week, fasted for 12 hours before the experiment, had free access to water, and ate uniformly after 8 hours of administration. The next day, the experimental animals were randomly divided into 2 groups, 10 in each group, administered with Shuangshen Pingfei Granules by intragastric administration respectively at doses of 5 g / kg and 15 g / kg, respectively at 5, 15, 30 min and 1, 1.5, 2, 4, 8, 12, and 24 hours, 0.3 mL of blood was collected from the venous plexus behind the eyes. The collected blood samples were collected in EP tubes filled with sodiumheparin, incubated in a 37°C water bath for 30 minutes, centrifuged at 5000rmp for 10 minutes, separated from the plasma, and stored...
[0122] 1. Administration and collection of plasma samples
[0123] The rats were fed adaptively for one week, fasted for 12 hours before the experiment, had free access to water, and ate uniformly after 8 hours of administration. The next day, the experimental animals were randomly divided into 2 groups, 10 in each group, and the above-mentioned Shuangshen Pingfei Granules were administered by intragastric administration at doses of 5g / kg and 15g / kg respectively. After administration, 5, 15, 30min and 1 , 1.5, 2, 4, 8, 12, and 24 hours, 0.3 mL of blood was collected from the venous plexus behind the eyes. The collected blood samples were collected in EP tubes filled with sodiumheparin, incubated in a 37°C water bath for 30 minutes, centrifuged at 5000rmp for 10 minutes, separated from the plasma, and stored in a -80°C refrigerator for testing.
[0124] 3.2 Pre...
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Abstract
The invention discloses a method for quantitatively detecting main components of Shuangshen Pingfei granules in plasma at the same time. According to the method, an ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) is used for rapidly detecting the contents of mangiferin, tanshinone IIA, ginsenoside RgI, salvianolic acid B, naringin, liquiritin, cryptotanshinone, timosaponin BII and glycyrrhizic acid in the rat plasma at the same time after oral medication of the Shuangshen Pingfei granules. The method comprises the preparation of standard substance stock solution, the preparation of internal standard working solution, the preparation of a standard curve, the processing of a plasma sample and the measurement of the contents. The method provided by the invention is high in sensitivity, strong in specificity, good in reproducibility and simple and rapid to operate, accords with the in-vivo biological sample measurement requirements and can be applied to the pharmacokinetic researches after oral medication of the Shuangshen Pingfei granules.
Description
technical field [0001] The invention relates to the field of medicine, in particular to the technical field of in vivometabolism research of Chinese patent medicines, in particular to a method capable of rapidly and simultaneously quantitatively detecting the main components of Shuangshen Pingfei Granules in plasma. Background technique [0002] Shuangshen Pingfei Prescription is a classic addition and subtraction prescription preferred by Professor Fan Xinsheng of Nanjing University of Traditional Chinese Medicine. The granules with the functions of clearing the lungs and moistening dryness, antitussive and anti-vomiting are used to treat pulmonary fibrosis caused by deficiency of qi and yin, obstruction of phlegm and blood stasis, and the clinical curative effect is remarkable. [0003] Studies have found that the main components of Shuangshen Pingfei Granules are saponins, phenolic acids, tanshinones, flavonoids and other compounds. Ginsenoside Rg1, tanshinone IIA, cryp...
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