Method for extracting and injecting adipose-derived stem cells

A technology of adipose stem cells and fat, which is applied in the field of extraction and injection of adipose stem cells, can solve problems such as puncture, affect the operation process, increase customer pain, etc., and achieve the effect of achieving precise injection, increasing the acquisition rate, and increasing the survival rate of backfilling

Inactive Publication Date: 2019-09-17
成都天府新区可纳儿医疗美容门诊部有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For this reason, in the prior art, it is often necessary to replace the small-sized syringe at the end of the injection in order to better control the injection volume, but this will cause repeated punctures, which not only affects the operation process, but also increases the pain of the customer.

Method used

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  • Method for extracting and injecting adipose-derived stem cells
  • Method for extracting and injecting adipose-derived stem cells
  • Method for extracting and injecting adipose-derived stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The extraction method of autologous adipose stem cells includes the following steps:

[0033] ① Collect 50ml of autologous fat on the abdomen or inner thigh. The collection method is suction. Since both the abdomen and inner thigh are the places where fat is easy to accumulate, the fat collection is convenient and can play a role in liposuction and weight loss. Of course, the collection volume of autologous fat should be selected according to the actual situation. The collection volume of a single site and a single time is not easy to be too large, otherwise it is prone to surface skin wrinkles and sagging.

[0034] After the collection is completed, inject the autologous fat into the collection cup and deposit it naturally in a sterile environment. After the swelling fluid and the fat tissue are naturally stratified, the lower layer of fluid is aspirated and then an equal volume of 0.9% normal saline is added. In the adipose tissue, shake the adipose tissue repeatedly and l...

Embodiment 2

[0038] The extraction protocol described in paragraphs 0016-0017 on page 2 of the patent technology CN103667187A specification is used as a control method to collect human adipose tissue and extract adipose stem cells. For the specific method, please refer to the patent technology CN103667187A.

Embodiment 3

[0040] Liposuction was performed from the body of volunteers, and the adipose stem cells were extracted according to the methods of Example 1 and Example 2. Repeated 4 times, 5 samples were extracted each time, and the number of autologous adipose stem cells and survival rate were counted. The results are detailed in the table 1.

[0041] Table 1

[0042]

[0043] According to Table 1, we can clearly see that the number and survival rate of adipose stem cells extracted in Example 1 were significantly higher than those in Example 2, and the experiment was repeated 4 times. The data between each group was similar, relatively stable, and sufficient Evidence supports the effect of the invention.

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Abstract

The present invention relates to the technical field of cosmetics and aims to provide a method for extracting and injecting adipose-derived stem cells. The method can improve extraction amount and survival rate of adipose and can achieve precise injection during an injection phase. A used technical scheme is as follows: cultured autologous adipose-derived stem cells are filled in an adipose precision syringe, and then injection is conducted in recipient areas in a first rough injection and then fine injection manner; a rough injection amount is obtained by reducing 5% based on an estimated injection amount and a fine injection amount is obtained by slow push of the syringe by cosmetic doctors after completion of the rough injection according to obtained actual effects based on the actual injection amount; and the estimated injection amount is obtained according to expected cosmetic effects. The method can improve acquisition rate and backfilling survival rate of the autologous adipose-derived stem cells, conveniently controls the injection amount of the autologous adipose and facilitates the precise injection.

Description

Technical field [0001] The present invention relates to the field of cosmetic technology, in particular to methods for extracting and injecting fat stem cells. Background technique [0002] Adipose-derivedstemcells (ADSCs) are a kind of stem cells with multi-differentiation potential isolated from adipose tissue in recent years. They have the advantages of rapid expansion and resistance to senescence. Autologous fat transplantation is one of the important items in the cosmetic project. It is widely used in facial plastic surgery, breast augmentation, buttocks augmentation and other plastic surgery. It has the advantages of no incision, no rejection, real shape and natural feel. [0003] The process of autologous fat transplantation mainly includes two major steps: one is the extraction of adipose stem cells, and the other is the injection of adipose stem cells. In the process of extracting adipose stem cells, traditional methods not only have low extraction rate and survival rate,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61M5/178A61M5/315
CPCA61M5/178A61M5/31511A61M5/31556A61M2202/09C12N5/0667C12N2509/00A61M2202/0021
Inventor 赵俊伟
Owner 成都天府新区可纳儿医疗美容门诊部有限公司
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