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Compounds with anticancer activity and preparation method thereof

An anti-cancer activity and compound technology, applied in the field of medicine, can solve problems such as hindering the development and utilization of the medicinal value of Caolong, and achieve the effect of high purity

Active Publication Date: 2019-09-20
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few researches on the chemical constituents and pharmacological effects of Aurora at home and abroad, which hinders the development and utilization of the medicinal value of Aurora.

Method used

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  • Compounds with anticancer activity and preparation method thereof
  • Compounds with anticancer activity and preparation method thereof
  • Compounds with anticancer activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for extracting a compound with anticancer activity, comprising the following steps:

[0046] 1) using solvent extraction method to extract the medicinal material Herba Aurora, and obtain the extract of Aurora Herba;

[0047]2) adding water to the Herba Dragon extract for suspension, extracting with petroleum ether to obtain a petroleum ether extract and a water isolate; adding ethyl acetate to the water isolate for extraction to obtain an ethyl acetate extract;

[0048] 3) Adsorbing the petroleum ether extract and the ethyl acetate extract with a macroporous resin respectively, and performing gradient elution with an alcohol-water mixed solution as an eluent to obtain a first fraction and a second fraction;

[0049] 4) Adsorbing the first fraction and the second fraction with a silica gel chromatographic column respectively, and performing gradient elution with dichloromethane-methanol mixed solvent as the eluent to obtain the third fraction and the fourth frac...

Embodiment 2

[0052] A method for extracting a compound with anticancer activity, comprising the following steps:

[0053] 1) Use 80% ethanol solution to impregnate the grass dragon with a solid-liquid ratio of 1:8 for 2 days, and filter to obtain the grass dragon extract;

[0054] 2) adding water to the Herba Dragon extract for suspension, extracting with petroleum ether to obtain a petroleum ether extract and a water isolate; adding ethyl acetate to the water isolate for extraction to obtain an ethyl acetate extract;

[0055] 3) Adsorb the petroleum ether extract and ethyl acetate extract with a macroporous resin respectively, use ethanol:water with a volume ratio of 1:9 to start eluting, and gradually increase the ratio of ethanol until the volume ratio of ethanol:water is 95 : 5, collect the cut that contains the first flow fraction and the second flow fraction, obtain the first flow fraction and the second flow fraction;

[0056] 4) Adsorb the first fraction and the second fraction wi...

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Abstract

The invention provides compounds with anticancer activity. The compounds have a general structural formula I as follows, wherein the R group is CH3 or H. The compounds are extracted from ludwigia hyssopifolia. The invention further provides a preparation method of the compounds. The preparation method comprises the following steps: 1) extracting medicine ludwigia hyssopifolia by using a solvent extraction method to obtain a ludwigia hyssopifolia extract; 2) separately extracting petroleum ether and ethyl acetate to obtain a first concentrate and a second concentrate; 3) separately adsorbing the petroleum ether extract and the ethyl acetate extract by using macroporous resin to obtain a first stream and a second stream; 4) separately adsorbing the first stream and the second stream by using a silica gel chromatographic column to obtain a third stream and a fourth stream; and 5) separately purifying the third stream and the fourth stream by adopting a preparative high-performance liquid chromatography method to obtain a compound with the R group of CH3 and a compound with the R group of H. The compounds have anti-tumor activity.

Description

technical field [0001] The present invention relates to the field of medicine. More specifically, the present invention relates to a compound with anticancer activity and its preparation method. Background technique [0002] Zhuang medicine Caolong (strong name: Gvahgya croak clip) is the dried whole herb of Ludwigia hyssopifolia (G.Don) Exell of the willow vegetable family. "Guangxi Zhuang Autonomous Region Zhuang Medicine Quality Standard" records that it has heat-clearing and poisonous properties, and can pass water. , the effect of reducing swelling and pain, used to treat diseases such as Ayimi (dysentery), infectious hepatitis, and stupid floating (edema). However, at present, there are few researches on the chemical constituents and pharmacological effects of Herba Astronaus at home and abroad, which hinders the development and utilization of Herba Dragon's medicinal value. Contents of the invention [0003] An object of the present invention is to solve at least ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D313/00A61P35/00A61K31/365
CPCA61P35/00C07D313/00
Inventor 卢汝梅张进燕霍丽妮韦建华黎云清
Owner GUANGXI UNIV OF CHINESE MEDICINE
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