KASP molecular marker method for identifying rice blast resistance gene Pigm

A rice blast and molecular marker technology, applied in the field of biotechnology engineering, can solve the problems of cumbersome detection process, poor polymorphism of molecular markers, and low level, and achieve the effects of improving selection efficiency and speeding up the breeding process.

Active Publication Date: 2019-09-20
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0010] Technical problem: The present invention aims at the time-consuming and labor-intensive breeding of rice blast resistance containing the traditional Pigm gene and is highly susceptible to environmental interference and the accuracy is not high, and the existing molecular markers have poor polymorphism in different parents, the accuracy is not high, and the detection process is relative cumbersome and other disadvantages

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  • KASP molecular marker method for identifying rice blast resistance gene Pigm
  • KASP molecular marker method for identifying rice blast resistance gene Pigm
  • KASP molecular marker method for identifying rice blast resistance gene Pigm

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Embodiment Construction

[0047] "A KASP molecular marker method for identifying rice blast resistance gene Pigm" its specific implementation steps are as follows:

[0048] (1) Experimental materials (the following materials are publicly known and public, and the medium-term bank of agricultural germplasm resources in Jiangsu Province can provide them to the outside world)

[0049] Materials containing Pigm homozygous genotype: Gumei No. 4, a Sichuan indica landrace.

[0050]Pigm-resistant homozygous genotype materials: four near-isogenic lines IRBL9-W(Pi9), IRBLz5-CA-1(Pi2), IRBLz-Fu(Piz), IRBLz-Fu(Piz), IRBLzt-T (Piz-t) (cited by International Rice Research Institute, IRRI), indica rice restorer line materials Zhenhui 084 (Zhenjiang Agricultural Science Research Institute of Jiangsu Hilly Region), 93-11 (Jiangsu Lixiahe Regional Agricultural Science Research Institute), Zhonghui 8006 (China Rice Research Institute-Zhejiang), Chenghui 727 (Crop Research Institute of Sichuan Academy of Agricultural Sc...

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Abstract

The invention relates to a KASP molecular marker method for identifying rice blast resistance gene Pigm and belongs to the technical field of bioengineering. KASP molecular marker primers are designed according to the fact that Gumei 4 and indica rice and sequenced round-grained nonglutinous rice varieties have G-C base mutation at upstream 515bp of Pigm site Pigm-R2 gene initiation codon, fluorescent quantitative fluorescent amplification is performed on the DNA of different rice materials, and the Pigm genotype of the rice materials is judged. The method has the advantages that whether rice germplasm resources or breeding population contains the Pigm gene or not can be fast and accurately identified, and high-throughput detection of multiple sample materials can be achieved at the same time; the method is fast and efficient as compared with the conventional molecular marker technology; the method can further increase the selection efficiency of Pigm-containing disease-resistant homozygous genotype during breeding and accelerate the breeding process of rice-blast-resistant rice varieties.

Description

[0001] 1. Technical field [0002] The invention relates to a KASP molecular marker method for identifying the rice blast resistance gene Pigm, which belongs to the field of biotechnology engineering and is specially used for the identification, screening and variety selection of rice blast resistance germplasm resources containing the Pigm gene. [0003] 2. Background technology [0004] Rice blast is a fungal disease caused by Ascomycetes (Magnaporthe oryzae (Hebert) Barr., asexual Pyriculariaoryae Cav.), and the global annual yield loss due to rice blast accounts for 10-30% of the total rice output (Wilson R A et al., Under pressure: investigating the biology of plantinfection by Magnaporthe oryzae, Nature Reviews Microbiology, 2009, 7(3):185-195). In my country, rice blast occurs to varying degrees every year, and the annual occurrence area is about 4.0×10 6 ~6.0×10 6 hm 2 , loss of paddy 6.0×10 5 ~1.45×10 6 t, has become an important factor restricting rice production...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2563/107C12Q2537/161C12Q2535/137
Inventor 陈涛张亚东梁文化张善磊孙旭超姚姝赵庆勇周丽慧赵春芳朱镇赵凌王才林
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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