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A technical method for increasing the number of eggs laid by female silkworm moths

A technology for quantity and female moths, applied in the direction of recombinant DNA technology, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of increasing the number of eggs laid by silkworm female moths and lack of research

Active Publication Date: 2021-03-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a lack of research on how the silkworm lipokinetic hormone receptor mediates the action of silkworm lipokinetic hormone, thereby regulating the energy distribution of the silkworm body, and increasing the number of eggs laid by the silkworm female moth.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The fat body of silkworm chrysalis on the third day of pupation was dissected and collected, and the total RNA was extracted using Trizol kit, and then the total RNA was reverse-transcribed with the first-strand cDNA synthesis reagent for reverse transcription to obtain the first-strand cDNA; the reverse transcription conditions were: Denature at 30°C for 10 minutes, react at 50°C for 60 minutes, and inactivate at 95°C for 5 minutes.

[0032] Using the first-strand cDNA as a template, the forward primer 5'-ATGGATATAGACGAGAAAGTGTC-3' and the reverse primer 5'-TTAACCATACCGTTCGTTACG-3' were used to amplify by reverse transcription polymerase chain reaction (RT-PCR), and cloned to obtain BmAKHR cDNA; the RT-PCR amplification conditions are: pre-denaturation at 94°C for 2min, denaturation at 94°C for 15s, annealing at 50°C for 30s, extension at 68°C for 80s, and 30 cycles.

[0033] Using BmAKHR cDNA as a template, the forward primer 5'-ATGGATATAGACGAGAAAGTGTCCG-3' and the re...

Embodiment 2

[0038] The fat body of silkworm chrysalis on the third day of pupation was dissected and collected, and the total RNA was extracted using Trizol kit, and then the total RNA was reverse-transcribed with the first-strand cDNA synthesis reagent for reverse transcription to obtain the first-strand cDNA; the reverse transcription conditions were: Denature at 30°C for 10 minutes, react at 50°C for 60 minutes, and inactivate at 95°C for 5 minutes.

[0039] Using the first-strand cDNA as a template, the forward primer 5'-ATGGATATAGACGAGAAAGTGTC-3' and the reverse primer 5'-TTAACCATACCGTTCGTTACG-3' were used to amplify by reverse transcription polymerase chain reaction (RT-PCR), and cloned to obtain BmAKHR cDNA; the RT-PCR amplification conditions are: pre-denaturation at 94°C for 2min, denaturation at 94°C for 15s, annealing at 50°C for 30s, extension at 68°C for 80s, and 30 cycles.

[0040] Using BmAKHR cDNA as a template, the forward primer 5'-ATGGATATAGACGAGAAAGTGTCCG-3' and the re...

Embodiment 3

[0045] The fat body of silkworm chrysalis on the third day of pupation was dissected and collected, and the total RNA was extracted using Trizol kit, and then the total RNA was reverse-transcribed with the first-strand cDNA synthesis reagent for reverse transcription to obtain the first-strand cDNA; the reverse transcription conditions were: Denature at 30°C for 10 minutes, react at 50°C for 60 minutes, and inactivate at 95°C for 5 minutes.

[0046] Using the first-strand cDNA as a template, the forward primer 5'-ATGGATATAGACGAGAAAGTGTC-3' and the reverse primer 5'-TTAACCATACCGTTCGTTACG-3' were used to amplify by reverse transcription polymerase chain reaction (RT-PCR), and cloned to obtain BmAKHR cDNA; the RT-PCR amplification conditions are: pre-denaturation at 94°C for 2min, denaturation at 94°C for 15s, annealing at 50°C for 30s, extension at 68°C for 80s, and 30 cycles.

[0047] Using BmAKHR cDNA as a template, the forward primer 5'-ATGGATATAGACGAGAAAGTGTCCG-3' and the re...

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Abstract

The invention discloses a technical method for increasing oviposition quantity of bombyx mori female moths. The technical method comprises the steps of performing extraction on bombyx mori pupa fat bodies to obtain total RNA, performing reverse transcription so as to obtain first-chain cDNA, performing RT-PCR amplification by using the first-chain cDNA as a formwork, performing cloning so as to obtain BmAKHR cDNA, performing PCR amplification by using the BmAKHR cDNA as a formwork, after enzyme cutting, connecting a DNA fragment to a carrier to obtain pFLAG-BmAKHR, then performing PCR amplification to obtain a reaction formwork, and performing further treatment to obtain BmAKHR siRNA; removing bacteria from BmAKHR siRNA liquid through a filter membrane, injecting the BmAKHR siRNA liquid after bacterium removal into abdomens of silkworms, raising the silkworms with mulberry leaves until the silkworms are mature and cocooned, and after the silkworms pupate to become moths, performing normal mating so that the quantity of produced silkworm ova. Through the adoption of the technical method disclosed by the invention, the oviposition quantity of the bombyx mori female moths can be increased through adjusting and controlling energy metabolism of bombyx mori instead of increasing the quantity of the mulberry leaves eaten by the silkworms, and the technical method has positive effects on researching the substance energy metabolism mechanism of the bombyx mori, further increasing the variety production quantity of the bombyx mori, further promoting the physiological functions of the bombyx mori and the like.

Description

technical field [0001] The invention relates to a technical method for increasing the number of eggs laid by female silkworm moths. Background technique [0002] The silkworm (Bombyx mori) originated in China and is the largest economic resource insect reared indoors on the earth today. Sericulture has a history of more than 5,000 years in my country as a characteristic and advantageous industry. At present, my country is still the largest sericulture country in the world. Bombyx mori absorb various nutrients by eating mulberry leaves in the larval stage, and supply various tissues and organs in silkworms at various stages through metabolic physiological processes for growth, development, reproduction, etc. Nutrients are supplied to ovaries for silkworm egg production. The large-scale breeding of silkworms is divided into two types: silkworm cocoon production and silkworm egg production. The main purpose of silkworm egg production is to obtain high-quality and high-yield si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/12A01K67/04
CPCA01K67/04A01K2227/706A01K2267/02C07K14/43586C12N15/85
Inventor 时连根沈张飞屠振力
Owner ZHEJIANG UNIV
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