Method for detecting anti-mold performance of antibacterial leather by ATP bioluminescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for detecting anti-mold performance of antibacterial leather. The method comprises detection steps as follows: preparation and pretreatment of samples; model selection of equipment and preparation of a reagent and a culture medium; collection and activation of strains and preparation of a spore solution; construction of an OD value-viable bacteria content log value lgCB standard curve; construction of an ATP concentration log value lgCA-relative fluorescence intensity log value igIA standard curve and viable bacteria ATP concentration CA calibration of an inoculation bacterial solution; sample inoculation, culture and elution recovery; IA determination of a recovery solution and calculation of viable bacteria ATP concentration CA and TA of the recovery solution; calculation of an antibacterial rate R or antibacterial activity value A; and result evaluation. The method is characterized by applying an ATP fluorophotometer to precisely and quantitatively test the anti-mold performance of the leather represented by R or A. The method elutes and recovers control samples and antibacterial samples after 0h contact and 48 h culture, determines IA of the recovery solution and represents and calculates R or A by lgIA. The researched and developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the anti-mold performance of the leather will support product quality improvement by the scientific and advanced detection technology.

Description

technical field [0001] The invention relates to a method for testing the antifungal properties of antibacterial leather, in particular to an ATP bioluminescence lgC for accurate and quantitative detection of antifungal properties of antibacterial leather characterized by antibacterial rate R or antibacterial activity value A by using an ATP fluorescence photometer A -lgI A The standard curve method belongs to the technical field of leather antibacterial function detection. Background technique [0002] Leather is a necessities of human life. Due to the introduction of a large number of fatliquoring agents in the tanning process and the raw hides used are rich in nutrients such as protein and fat, they are highly susceptible to bacteria, fungi and other microorganisms. With the improvement of people's living standards and the enhancement of health awareness, the requirements for antibacterial properties of leather products continue to rise; antibacterial leather came into be...

AI Technical Summary

Problems solved by technology

In general, the existing anti-mold performance testing methods have the following common problems in technical content and practical application: First, because the mycelium formed during the growth of mold spores cannot be accurately counted, only qualitative judgments can be made, and quantitative tests cannot be performed; The second is that the mold growth cycle is long, and the cultivation time is at least 2 to 4 weeks, resulting in a long test cycle and high time and economic costs; the third is that the experimental process is cumbersome and technically difficult; related operations are greatly affected by human factors, making Large test error, lack of comparability

Driven by the demand, foreign antibacterial material performance testing technology research field aimed at the current development trend of quantification, rapidity and simplification, has drawn on the principle of ATP fluorescence analysis to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity oftextile products (textiles- Determination of Antibacterial Performance of Antibacterial Finished Textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorescence assay to characterize anti-bacteria / mold performance, but it is only applicable to textile or microporous materials with water absorption and control sample / mold growth value > 0, in key technical aspects such as viable bacteria recovery method and effective test conditions Not applicable to leather, plastic and other materials with non-absorbent hard surface and mold growth value of the control sample <0, and no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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