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Immobilization method of β-glucosidase

A technology of glucosidase and nano-silicon carbide is applied in the field of immobilization of β-glucosidase, which can solve the problems of low repeated utilization rate of β-glucosidase, low enzyme activity retention rate, etc., so as to solve the problem of low repeated utilization rate. , Solve the effect of low enzyme activity retention rate and increase immobilization capacity

Active Publication Date: 2021-01-12
SHAANXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] An object of the present invention is to provide a method for immobilizing β-glucosidase, which improves the activity of β-glucosidase and solves the technical problems of low reutilization rate and low enzyme activity retention rate of β-glucosidase

Method used

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  • Immobilization method of β-glucosidase
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  • Immobilization method of β-glucosidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The invention provides a method for immobilizing β-glucosidase, comprising the following steps:

[0058] S01 preparation of nano-silicon carbide carrier includes: taking 0.5g of nano-silicon carbide powder (average particle diameter 40 nanometers) raw material, adding 80mL concentration of 30% hydrogen peroxide, magnetic stirring, 105 ℃ constant temperature reaction for 4h, 9000rpm centrifugation for 10min, and using After repeated washing with distilled water to neutrality, and vacuum drying at 80°C for 12 hours, hydroxylated nano-silicon carbide powder was obtained; Fourier transform infrared spectrometer (FTIR) was used to analyze the raw materials of nano-silicon carbide and hydroxylated nano-carbides. Silicon powder is tested, such as figure 1 Shown:

[0059] Nano-silicon carbide raw materials only contain a small amount of groups such as carboxyl and hydroxyl groups. The present invention uses nano-silicon carbide as an immobilized material, and needs to activate...

Embodiment 2

[0067] The invention provides a method for immobilizing β-glucosidase, comprising the following steps:

[0068] S01 preparation of nano-silicon carbide carrier includes: taking 0.5g of nano-silicon carbide powder (average particle diameter 40 nanometers) raw material, adding 80mL concentration of 30% hydrogen peroxide, magnetic stirring, 105 ℃ constant temperature reaction for 4h, 9000rpm centrifugation for 10min, and using After repeated washing with distilled water to neutrality, and vacuum drying at 80°C for 12 hours, hydroxylated nano-silicon carbide powder was obtained; Fourier transform infrared spectrometer (FTIR) was used to analyze the raw materials of nano-silicon carbide and hydroxylated nano-carbides. Silicon powder is tested, such as figure 1 Shown: See Example 1 for details figure 1 instruction of;

[0069] To activate the hydroxylated nano-silicon carbide, take 0.5 g of the hydroxylated nano-silicon carbide powder and dissolve it in a sodium acetate buffer sol...

Embodiment 3

[0076] The invention provides a method for immobilizing β-glucosidase, comprising the following steps:

[0077] S01 preparation of nano-silicon carbide carrier includes: taking 0.5g of nano-silicon carbide powder (average particle diameter 40 nanometers) raw material, adding 80mL concentration of 30% hydrogen peroxide, magnetic stirring, 105 ℃ constant temperature reaction for 4h, 9000rpm centrifugation for 10min, and using After repeated washing with distilled water to neutrality, and vacuum drying at 80°C for 12 hours, hydroxylated nano-silicon carbide powder was obtained; Fourier transform infrared spectrometer (FTIR) was used to analyze the raw materials of nano-silicon carbide and hydroxylated nano-carbides. silicon powder for detection, figure 1 See Example 1 for explanation figure 1 explanation of;

[0078] To activate the hydroxylated nano-silicon carbide, take 0.5 g of the hydroxylated nano-silicon carbide powder and dissolve it in a sodium acetate buffer solution w...

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Abstract

The invention belongs to the technical field of immobilization of biological enzymes, and particularly relates to an immobilization method of beta-glucosidase. The immobilization method comprises the following steps: preparing a nanometer silicon carbide carrier; mixing the beta-glucosidase, the nanometer silicon carbide carrier and a crosslinking agent to obtain immobilized beta-glucosidase; and mixing the immobilized beta-glucosidase and Fe3O4 nanoparticles to obtain a nanometer silicon carbide@ beta-glucosidase@Fe3O4 complex. By the immobilization method, the activity of the beta-glucosidase is improved and the technical problems of low beta-glucosidase reutilization rate and low enzyme activity retention rate are solved.

Description

technical field [0001] The invention belongs to the technical field of immobilization of biological enzymes, and in particular relates to an immobilization method of β-glucosidase. Background technique [0002] β-glucosidase (β-D-Glucosidase, EC3.2.1.21), also known as β-D-glucoside glucohydrolase, is also known as gentiobiase, cellobiase and amygdalinase. It belongs to cellulase and is an important component of cellulolytic enzyme system. It mainly acts on the β-(1,4) glycosidic bond, and can hydrolyze the non-reducing β-D-glucose bond bound to the terminal, while releasing Out of β-D-glucose and the corresponding ligand. β-glucosidase can also act on the glycosidic bond between the glycosyl atomic group and the aromatic group or hydrocarbon group to degrade the corresponding glycoside compounds to generate ligands and glucose. Another main application of β-glucosidase is to degrade cellulose. By cracking the cell wall rich in fiber, the nutrients contained in it, such as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N9/42
CPCC12N9/2445C12N11/14C12Y302/01021
Inventor 邓勖李日升张逸美党琳惠媛
Owner SHAANXI UNIV OF CHINESE MEDICINE