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Unwinding albumin hydrogel and preparation method thereof

A technology of hydrogel and albumin, applied in the field of medical hydrogel, can solve the problems of poor biocompatibility of hydrogel and poor mechanical properties of hydrogel, and achieve non-toxic raw materials, low synthesis cost and biocompatibility good sex effect

Inactive Publication Date: 2019-10-08
TIANJIN UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art. In order to solve the problem of poor mechanical properties of hydrogels prepared from natural polymers and poor biocompatibility of hydrogels prepared from synthetic polymers, it is proposed to use BSA as a raw material. Method for preparing injectable hydrogel from raw materials

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  • Unwinding albumin hydrogel and preparation method thereof
  • Unwinding albumin hydrogel and preparation method thereof
  • Unwinding albumin hydrogel and preparation method thereof

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0023] Choose PBS buffer with pH=8.0 to dissolve and react urea, bovine serum albumin and glutathione, first prepare and characterize the unwound structure of BSA, and use Jobin Yvin Fluorolog 3-21 steady-state fluorescence spectrometer for characterization , the specific process is as follows: (1) dissolve urea with PBS of pH = 8.0, and the urea concentration is 0-9M; (2) dissolve 10mgBSA in 1ml of urea solutions with different concentrations, and shake for 10min; (3) take 1ml ( 2) Add 0.003 g of glutathione to the BSA solution in , and shake for 10 minutes; (4) Test the maximum emission wavelength and absorption intensity of the solution under the condition of an excitation wavelength of 280 nm. Such as figure 1 As shown, it can be seen from the figure that the maximum emission wavelength of the absorption peak does not change significantly when the ...

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Abstract

The invention discloses unwinding albumin hydrogel and a preparation method thereof. Urea serves as a denaturing agent, glutathione serves as a reducing agent, and an intrachain disulfide bond in a bovine serum albumin is reduced into sulfydryl, so that the sulfydryl is reassembled into inter-chain disulfide bond. The hydrogel prepared by the method has good biocompatibility, is simple in preparation process and good in mechanical property and can be injected under physiological conditions.

Description

technical field [0001] The invention belongs to the field of medical hydrogels with good biocompatibility, and more specifically relates to a helicin hydrogel prepared by using urea as a denaturant and glutathione as a reducing agent and a preparation method thereof. Background technique [0002] Hydrogel has a three-dimensional network structure and high water content, which has strong variability. In situ injectable hydrogel can minimize surgical trauma and has been widely used in drug delivery and regenerative medicine. Materials used to make gels can be divided into natural materials and synthetic materials, but natural materials often have poor mechanical properties due to their own structural limitations. In recent years, in order to change this situation, researchers usually adopt two methods: one is to prepare double network hydrogels by combining two proteins or proteins with other macromolecules, and the other is to design polypeptides to self-assemble to form hydr...

Claims

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Application Information

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IPC IPC(8): C08J3/075A61L27/52A61L27/22A61L27/50
CPCA61L27/227A61L27/50A61L27/52A61L2400/06C08J3/075C08J2389/00C08L89/00
Inventor 赵瑾白钰李思迪李学平侯信原续波
Owner TIANJIN UNIV
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