PCR fluorescence detecting device and detection method thereof

Abstract

The invention relates to the technical field of PCR detection, and especially relates to a PCR fluorescence detecting device and a detecting method. The device solves the problems that a temperature circulation device and a flow rate control mechanism of the existing PCR fluorescence detecting device have complicated structure and high cost. The PCR fluorescence detecting device of the present invention comprises: a droplet generating unit, a micro-pipe, a temperature control unit, a collecting unit, a flow rate adjusting unit, and a fluorescence detecting unit. The droplet generating unit, the micro-pipe, the temperature control unit, the collecting unit, and the flow rate adjusting unit are sequentially connected, and the fluorescence detecting unit is located between the temperature control unit and the collecting unit. The fluorescence detecting method of the present invention comprises the steps of: pressurizing a sample in a sealed container to flow the sample into the micro-pipe; realizing temperature cycling of the sample by temperature differences at different locations of the temperature control unit; adjusting the flow rate of the sample; and detecting the sample in themicro-pipe. The method and the device can be used for DNA amplification reaction detection.

Description

technical field [0001] The invention relates to the technical field of PCR detection, in particular to a PCR fluorescence detection device and a detection method thereof. Background technique [0002] PCR (Polymerase Chain Reaction) is the abbreviation of polymerase chain reaction. After the DNA or RNA solution is diluted in large quantities by microfluidic method, it is dispersed into micro-droplets. The number of RNA templates in each droplet is less than or equal to one. DNA will denature at about 95°C, and decompose from double-stranded DNA into two single-stranded DNA; when the decomposed single-stranded DNA drops to about 60°C, the single-stranded DNA will bind to the primer; Under the action of DNA polymerase, semi-conservative replication is carried out according to the principle of complementary base pairing, and twice as much DNA can be obtained after the replication is completed. Therefore, after multiple cycles of temperature at 95°C and 60°C, a large amount of ...

AI Technical Summary

Problems solved by technology

This method is the most widely used, but it requires more sophisticated sensors and a complex temperature control system, which is costly and has a limited service life

[0003] In addition, the flow rate of liquid droplets needs to be precisely controlled during the reaction process, but the existing continuous flow PCR equipment uses a complex control system to control the liquid flow rate, the equipment structure is complex, the operation process is cumbersome, and the cost is high

Images