Method for detecting nucleic acid molecules based on cyclic amplification and cation exchange

Abstract

The invention belongs to the technical field of nucleic acid molecule detection, and discloses a method for detecting nucleic acid molecules based on cyclic amplification and cation exchange. The method comprises the following steps that a 3D nanometer walking machine is adopted to identify miRNA141 and carry out shearing amplification, magnetic beads and a shearing enzyme circulation technology are combined to release silver nanoparticles, the nanoparticles are converted to ions by an acid, cation exchange is performed with a photoelectric material CdSe, so that the CdSe photoelectric signalis reduced, and the detection of the miRNA141 is realized. The material with photoelectrochemical activity of the invention has the advantages that under the action of light, the outer electrons absorb energy to generate transitions to transfer charge, the electrode material is modified; and the material with photoelectrochemical activity can be combined with DNA, antibody, nano-gold and other substances, the application range of a photoelectrochemical sensor is greatly enriched, and the material with photoelectrochemical activity can also be combined with PCR, enzyme-linked reaction, cyclic amplification and other methods to improve the sensitivity of detection.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid molecule detection, and in particular relates to a method for detecting nucleic acid molecules based on cycle amplification and cation exchange. Background technique [0002] At present, the closest existing technology: at present, the detection of miRNA is closely related to the occurrence of tumors, so it serves as an important basis for early diagnosis of tumors. Existing fluorescence, aggregation amplification and imprinting techniques are not sensitive enough for detection and have poor specificity. Photoelectric detection technology is widely used in the detection of nucleic acid molecules due to its good stability, low background signal and wide detection range. At present, the method for detecting miRNA is mainly to combine DNA molecular machines with electrochemiluminescence sensing. First, the quantum dots are brought close to the gold nanoparticles, and resonance energy transfer o...

AI Technical Summary

Problems solved by technology

[0008] (1) Current 3D DNA molecular machines use metal particles as substrates and immobilize DNA probes on their surfaces. If the probes are disorderly and disorderly arranged, there are also many non-specific adsorptions between metal particles and probes, resulting in 3D DNA nanomachines operate less efficiently

[0009] (2) The sensitivity of existing nucleic acid molecular detection is low

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