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Blood donepezil drug concentration monitoring kit and detection method thereof

A technology of donepezil and kit, which is applied in the field of blood donepezil therapeutic drug monitoring kit, can solve the problems of high technical requirements for experimenters, inaccurate qualitative and quantitative, complex pre-treatment, etc., to reduce errors and personnel operation errors, and improve the detection process Simple and fast, the effect of reducing the cost of experiments

Inactive Publication Date: 2019-10-22
苏州艾迪迈医疗科技有限公司
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  • Claims
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AI Technical Summary

Problems solved by technology

Previous methods have problems such as complicated pre-treatment, inaccurate qualitative and quantitative results, high cost, long analysis time, or high technical requirements for experimenters.

Method used

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  • Blood donepezil drug concentration monitoring kit and detection method thereof
  • Blood donepezil drug concentration monitoring kit and detection method thereof
  • Blood donepezil drug concentration monitoring kit and detection method thereof

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Embodiment Construction

[0035] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with illustrations and specific embodiments.

[0036] One, the preparation of donepezil therapeutic drug monitoring kit:

[0037] Standard curve reagent: Donepezil is formulated with animal serum containing 2% stabilizer and 2% plasticizer to prepare a series of concentrations, respectively 0.010mg / L, 0.030mg / L, 0.050mg / L, 0.100mg / L, 0.150mg / L, 0.250mg / L, 1.0ml per bottle. The above components were freeze-dried and stored at the bottom of the assay bottle.

[0038] Quality control reagents: donepezil is formulated with animal serum containing 2% stabilizers and 2% plasticizers to three concentrations of high, medium and low, respectively 0.020mg / L, 0.080mg / L, and 0.200mg / L. Contains 1.0ml. The above components were freeze-dried and stored at the bottom of the assay bottl...

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Abstract

The invention relates to the field of medical detection and in particular to a blood donepezil therapeutic drug monitoring kit developed based on multi-dimensional online solid-phase extraction liquidchromatography analytical technology, more specifically to a method for accurately and quantitatively detecting donepezil drug concentration in blood by online solid-phase extraction and multi-dimensional liquid chromatography analytical technology. The kit includes a calibration reagent, a quality control reagent, a processing liquid, an extract liquid, a washing liquid, and an eluent. The therapeutic drug monitoring kit of the invention only needs to centrifuge a blood sample, achieves low repeated detection variability, high accuracy and stability, and low detection cost, has various methodological indicators that satisfy the requirement for monitoring the blood donepezil therapeutic drugs, and is easy to generalize.

Description

technical field [0001] The present invention relates to the field of medical testing, in particular to a kit for monitoring donepezil therapeutic drug in blood developed based on multi-dimensional online solid-phase extraction liquid chromatography analysis technology, more specifically, it adopts online solid-phase extraction and multi-dimensional liquid chromatography analysis A method for accurate and quantitative monitoring of donepezil drug concentration in blood. Background technique [0002] Donepezil belongs to hexapyridine oxides and is a second-generation specific reversible central acetylcholinesterase (AChE) inhibitor. The mechanism of action of donepezil is to reversibly inhibit acetylcholinesterase (AchE), reduce the hydrolysis of acetylcholine, and increase the content of acetylcholine at the receptor site. There may be other mechanisms, including effects on peptides, direct effects on neurotransmitter receptors, or Ca2+ channels. The effective blood concent...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/16G01N30/34G01N30/86
CPCG01N30/06G01N30/16G01N30/34G01N30/8631
Inventor 石功名何伶秀孙丹
Owner 苏州艾迪迈医疗科技有限公司
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