Application and preparation method of roe polypeptide

A technology for roe and application, applied to the application of roe polypeptide and the field of preparation thereof, can solve the problems of toxic and side effects, hemolysis, unsatisfactory specificity and activity of anti-tumor polypeptide, etc., and achieve the effects of alleviating physical pain and high safety and reliability.

Inactive Publication Date: 2019-11-01
SUN YAT SEN UNIV +1
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  • Claims
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Problems solved by technology

Many antibacterial peptides with antitumor activity have been clinically tested, among which lactoferrin (Lactoferrin) has been used in the treatment of lung non-small cell carcinoma; however, due to the diversity of tumor cell types, the same peptide is not effective for all tumors The cells are all effective. Moreover, some anti-tumor polypeptides are not ideal in terms of specificity and activity, and have toxic side effects such as hemolysis, so the screening of polypeptides with anti-tumor functions should continue to be strengthened; in addition, for anti-tumor experiments. detection, the detection results are not ideal and accurate, and it is necessary to strengthen the operability of the experiment and the accuracy of the data

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  • Application and preparation method of roe polypeptide
  • Application and preparation method of roe polypeptide
  • Application and preparation method of roe polypeptide

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specific Embodiment approach

[0025] A use of caviar polypeptide, the use is in the preparation of anti-tumor, prevention and treatment of tumor food, health products or medicines, the active ingredient of the caviar polypeptide is fish egg extract.

[0026] A preparation method of caviar polypeptide, the preparation method mainly includes the following steps:

[0027] 1) First, collect fish egg cells; fish egg cells can be obtained from deep-sea sturgeon roe;

[0028] 2) Secondly, the fish egg cells are sequentially washed → cell homogenate → freeze-thaw → precipitation → supernatant taken → interception and removal of impurities → detection; the molecular weight cut-off for interception and removal of impurities is 10,000 Daltons;

[0029] 3) Next, the caviar polypeptide stock solution is obtained after filtering and sterilizing;

[0030] 4) Freeze-drying and concentrating the caviar polypeptide stock solution to prepare caviar polypeptide powder;

[0031] 5) Prepare caviar polypeptide high-concentrati...

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Abstract

The invention relates to the field of antitumor food, in particular to application and a preparation method of roe polypeptide. The application refers to application of the roe polypeptide in preparation of antitumor and tumor prevention and treatment food. The preparation method of the roe polypeptide comprises the steps as follows: 1) collection of roe cells; 2) thorough cleaning, cell homogenization, freezing and thawing, sedimentation, taking of supernatant, interception and impurity removal and detection treatment of the roe cells; 3) preparation of a roe polypeptide stock solution; and 4) preparation of roe polypeptide powder, mother liquor or solution. Roes play antitumor and anticancer functions by the inhibition effect on melanoma and tumor. Fish byproducts are efficiently utilized in medicines and food and create social benefits for economic development. Compared with a traditional antitumor drug preparation, as bioactive peptides naturally exist in the roe polypeptide stocksolution obtained by roe extraction, the components of the roe polypeptide have certain safety, as an antitumor drug or health food, the roe polypeptide has safety and reliability higher than that oftraditional antitumor drug preparation.

Description

technical field [0001] The invention relates to the field of an anti-tumor food, in particular to the use of caviar polypeptide and its preparation method. Background technique [0002] In recent years, anti-tumor drugs have become a topic of concern to modern people, and their research and development work has also attracted public attention. As we all know, malignant tumors seriously threaten human life, health and safety, but most of the current anti-tumor drugs cannot completely resist viruses. , and there are disadvantages such as low selectivity, strong toxic and side effects, and easy drug resistance. Therefore, the research and development of new anti-tumor drugs has always been the focus of research and development in the biological field. At present, research has found that antimicrobial peptides have broad-spectrum antibacterial activity and can quickly kill targets, and many of them are pure natural peptides, making them quickly become potential therapeutic drugs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/04A23J3/04A23L33/18A61K38/02A61P35/00C07K1/36C07K1/34C07K1/30
CPCA23J1/04A23J3/04A23V2002/00A61K38/02A23L33/18A61P35/00C07K1/30C07K1/34C07K1/36A23V2200/308A23V2250/55
Inventor 陆家海郭中敏潘兴华薛攀攀李载红
Owner SUN YAT SEN UNIV
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