8results about How to "Achieve curative effect" patented technology

The invention relates to the technical field of medicinal chemistry and pharmacotherapeutics, in particular to a technology and a method for confirming toxic targets of evodiamine (EVO) and accurately evaluating hepatotoxicity of evodiamine. The method comprises the following steps: determining that EVO can be directly combined through biotin labeling, pulldown, mass spectrum combination, small molecule transfection and the like, and up-regulating liver zo-1 protein to cause liver injury, while up-regulating the intestinal tract target is a key mechanism for treating the inflammatory bowel disease by EVO. By comparing the protein levels in the liver-intestine axes of animals in different states, the fact that the down-regulation of the ZO-1 protein level in the liver-intestine axis under the inflammatory bowel disease state can partially antagonize the EVO to the up-regulation of the liver ZO-1 is found, so that the liver toxicity is reduced, and only the therapeutic activity of the EVO is retained. The discovery provides an accurate target spot for understanding an EVO toxicity mechanism, and is beneficial to realizing accurate regulation and control in drug research and development and toxicity prevention in the future; meanwhile, the drug effects and toxic mechanisms of EVO in organisms in different states are compared from the aspect of ZO-1 protein expression in the liver-intestine axis, and direct evidence is provided for the theory of combating poison with poison in toxic traditional Chinese medicine.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for improving lung deficiency type primary dysmenorrhea and a preparation method and application thereof. The traditional Chinese medicine composition specifically comprises multiple traditional Chinese medicine components including rhizoma polygonati, Chinese yam, rhizoma smilacis glabrae, radix salviae miltiorrhizae, yam rhizome, codonopsis pilosula, radix ophiopogonis from Sichuan of China, exocarpium citri grandis, spina date seeds, radix bupleuri, radix clematidis, morinda officinalis, rhodiola rosea, peach kernels, caulis spatholobi, southern apricot kernels and prepared rehmannia roots. The composition effectively relieves pain and improves accompanying symptoms through multi-target synergistic effects of promoting blood circulation to remove blood stasis, reinforcing qi and nourishing lung, warming kidney and strengthening spleen, regulating qi and relieving pain and the like. The formula follows the monarch, minister, assistant and guide theory of traditional Chinese medicine, is rigorous in compatibility, can comprehensively regulate qi, blood and viscera functions of a patient, is especially suitable for dysmenorrhea caused by deficiency of lung qi and unsmooth qi and blood circulation, and shows the advantages of overall treatment of traditional Chinese medicine. The preparation method is stable in process and easy to implement, and a safe and effective new choice for traditional Chinese medicine treatment is provided for lung deficiency type primary dysmenorrhea.

The invention belongs to the technical field of biomedicine, and particularly relates to a preparation method and application of a double-targeting bionic nano diagnosis and treatment agent, the preparation method of the double-targeting bionic nano diagnosis and treatment agent comprises the following steps: step 1, synthesis of MnFe2O4 nanoparticles; step 2, loading DHA (docosahexaenoic acid) on the MnFe2O4 nano particles, so as to prepare a MnFe2O4-DHA nano drug; 3, the outer side of the MnFe2O4-DHA nano drug is wrapped with a GL261 cell membrane, and MnFe2O4-DHA (at) CM is prepared. The nano-drug can effectively penetrate through BBB and is actively targeted to a tumor site, so that the MnFe2O4-DHA nano-drug is accurately released in cells, tumor marker targeted therapy is realized, H2O2 is efficiently catalyzed by the synthesized bionic nano-drug into toxic dual ROS-hydroxyl free radicals and singlet oxygen, tumor cells are killed, and the tumor marker targeted therapy is realized. An in-vivo anti-oxidation system is jointly inhibited inside and outside, a cascade amplification ferroptosis treatment effect is generated, and a relatively good killing effect on tumors can be achieved.

This invention relates to the field of biomedical technology, providing a method for preparing a drug-eluting microsphere suspension, the drug-eluting microsphere suspension, and its applications. The method includes: S1. Removing the storage solution from the drug-eluting microspheres to obtain a microsphere precipitate; S2. Adding a chemotherapy drug solution to the microsphere precipitate to load the drug into the microspheres; S3. Mixing physiological saline and a non-ionic contrast agent at a volume ratio of 1.5:1 to prepare a dilution medium; S4. Adding the dilution medium prepared in step S3 to the microspheres after drug loading in step S2, so that the total suspension volume reaches a preset multiple of the microsphere precipitate volume, obtaining a microsphere suspension; S5. Shaking the microsphere suspension obtained in step S4 to mix thoroughly. This invention significantly reduces postoperative chemical stimulation pain by increasing the dilution factor of the drug-eluting microsphere suspension, while unexpectedly improving tumor response, achieving a synergistic effect of pain reduction and efficacy enhancement.

The application discloses a kind of cyclic peptide radioactive tracer targeting tumor HER2, labeled precursor and application, it is related to the field of biological medicine.The labeled precursor is A-B-C structure, A is chelating agent, B is connecting arm, C is cyclic peptide compound, the amino acid sequence of cyclic peptide compound is as shown in SEQ ID NO:1, and its 1st cysteine and 10th cysteine form cyclization structure by disulfide bond;Cyclic peptide radioactive tracer includes nuclide and labeled precursor;The application also provides the application of labeled precursor or cyclic peptide radioactive tracer in preparing labeled diagnostic or therapeutic nuclide or HER2 high expression tumor diagnosis and treatment product.The radioactive tracer prepared in the application has good in-vivo stability, has high binding specificity with tumor HER2, has longer tumor retention time, and is simple to synthesize, and has important significance for promoting the clinical application of HER2 targeted polypeptide in nuclear medicine tumor diagnosis and treatment integration.

The invention provides a traditional Chinese medicine composition for preventing and treating postoperative recurrence of liver cancer, and relates to the technical field of traditional Chinese medicine pharmacy. The traditional Chinese medicine composition is prepared from coix seeds, cassia twigs, herba patriniae, oldenlandia diffusa, radix salviae miltiorrhizae, selfheal and pericarpium citri reticulatae, all the medicines are combined to achieve the effects of clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, reducing swelling and removing stasis and strengthening the spleen and excreting dampness, and the curative effect of preventing and treating postoperative recurrence of liver cancer is achieved. The traditional Chinese medicine composition disclosed by the invention has a remarkable inhibiting effect on proliferation and migration of liver cancer cells, and can be used for remarkably inhibiting tumor weight and tumor volume of a liver cancer postoperative recurrent mouse model, reducing the abdominal girth of the model mouse, reducing the number of liver nodules, reducing the AFP level in serum and improving the pathological change condition of liver para-carcinoma tissues. The traditional Chinese medicine composition has the effect of preventing postoperative recurrence of liver cancer and can be used as a clinical treatment medicine for postoperative recurrence of liver cancer.

This invention belongs to the field of physical therapy technology for Parkinson's disease, and provides a non-invasive closed-loop feedback vagus nerve electrical stimulation system for the prevention and treatment of Parkinson's disease. The system includes: a high-frequency electrical stimulator hardware subsystem, transcutaneous nerve stimulation electrodes, a respiratory and heart rate signal acquisition and closed-loop feedback subsystem, and a high-frequency electrical stimulator software subsystem. This invention uses kilohertz stimulation pulses to bidirectionally modulate the vagus nerve, activating afferent C fibers and initiating the anti-inflammatory neural pathway of locus coeruleus noradrenergic neurons, thus alleviating central inflammation. Simultaneously, it blocks A and B efferent fibers, inhibiting mediated α-synuclein retrograde axonal transport. Through multimodal physiological signal monitoring and embedded intelligent algorithms, it achieves real-time, closed-loop, and personalized control of stimulation parameters and automatic switching of stimulation modes, optimizing efficacy and improving safety. Through adhesive electrodes and acquisition devices, it achieves completely non-invasive, wearable, and home-based treatment.