An electrochemiluminescent microfluidic chip with a shared bipolar electrode cathode and its application
A microfluidic chip and bipolar electrode technology, which is applied in the field of microfluidic analysis, can solve the problems of reducing the accuracy and increasing the difficulty of luminescence detection, and achieves the effects of high accuracy, few types of reagents, and simple operation process.
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Embodiment 1
[0050] An ECL microfluidic chip (referred to as chip A in this embodiment) that shares the cathode of the bipolar electrode, its production and detection application processes are as follows:
[0051] (1) Use Adobe Illustrator CS5 drawing software to design the configuration of chip A, use carbon screen printing technology to make cloth chip electrodes (that is, driving electrodes and C-BPE), and wax screen printing technology to make the dams that constitute the cloth chip reaction pool.
[0052] The structure of the resulting chip is as figure 1 As shown, the integrated bipolar electrode on the chip has three branches, one of which is the cathode 1 of the bipolar electrode, and the other two branches are the anodes 2 and 3 of the bipolar electrode;
[0053] In one-to-one correspondence with the cathode and anode of the bipolar electrode, the positive electrode 4, negative electrode 5 and 6 of the driving electrode are distributed around the integrated bipolar electrode;
[...
Embodiment 2
[0064] An ECL microfluidic chip (called chip B in this embodiment) that shares the cathode of the bipolar electrode. On the basis of chip A, chip B increases the number of C-BPE anodes, and the manufacturing and detection process is similar to chip A.
[0065] image 3 It is a schematic diagram of the structure of chip B, there are 7 reporting reaction pools 2-8 and 1 supporting reaction pool 1.
[0066] Set up several experimental groups to investigate the ECL intensity when the same concentration of glucose is detected on chip B at the same time, modify 4.5U / μL GOD on the C-BPE anode a-g of the chip (corresponding to the reporting reaction pool 2-8 in turn), and report the reaction pool 2-8 A mixed solution containing 1 mM Luminol and 0.4 mM glucose was added respectively, and 10×PBS buffer solution was added to support reaction cell 1. The experiment was repeated five times, and the relationship between the ECL intensity value and the glucose concentration was as follows: ...
Embodiment 3
[0069] An ECL microfluidic chip (called chip C in this embodiment) that shares the cathode of the bipolar electrode is improved on the basis of chip B;
[0070] Figure 4 It is a schematic diagram of the structure of chip B, there are 7 reporting reaction pools 2-8 and 1 supporting reaction pool 1.
[0071] When chip B is used for multiple detection, 8 wires need to be connected at the same time to drive C-BPE to work. In order to make the operation of the chip easier and more efficient without affecting the function of the chip B, chip C can be obtained by connecting the negative electrodes of the 7 driving electrodes of the chip B with a ring electrode.
[0072] When chip C is used for multi-element detection, only 2 wires need to be connected to drive C-BPE to work. The negative pole of the stabilized power supply is connected to the position of the negative pole of the driving electrode facing the periphery of the reaction cell 5, and the positive pole is connected to the...
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