Engineering nucleic acid, T cell, and applications and production method thereof

An engineering and nucleic acid technology, applied in the field of biomedicine, can solve problems such as failure to achieve results, achieve strong anti-tumor effects and reduce T cell exhaustion.
CN110423757AActive Publication Date: 2019-11-08GUANGDONG TCRCURE BIOPHARMA TECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGDONG TCRCURE BIOPHARMA TECHNOLOGY CO LTD
Publication Date
2019-11-08

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Abstract

The invention discloses an engineering nucleic acid, which comprises nucleic acid of an antigen acceptor modified by gene engineering and can specifically binds an antigen from HPV; a nucleic acid ofan immune checkpoint acceptor for preventing tumor expression, wherein the nucleic acid can code one block protein; an engineering T cell, which comprises the above mentioned nucleic acid sequence; anapplication of the engineering T cell in the preparation of drugs for treating cancers; and a method for producing the engineering T cell. According to the method, a carrier is introduced into a T cell to obtain a TCR-T cell, then amplification is carried out to obtain the engineering T cell, and the carrier comprises the nucleic acid. The invention provides an engineering nucleic acid, a T cell,and an application and production method thereof. The obtained T cell can be used to recognize HPV E6 tumor antigen and can secrete a single chain antibody that blocks programmed cell death of protein PD-1. The engineering T cell has a strong antitumor function, the failure of T cell is reduced, and the nucleic acid and the T cell can be applied to immunological therapy of cancers of HPV E6 expression.
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Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to an engineered nucleic acid, T cells and their application and production method. Background technique

[0002] Some cancers, such as cervical cancer, are primarily caused by human papillomavirus (HPV) infection. Despite the effectiveness of treatments such as chemotherapy, many cancers, including HPV-related cancers, can have a poor prognosis. Thus, there is a medical need for cancer, especially HPV-related cancers.

[0003] HPV16 is a subtype of HPV that often causes malignant tumors. HPV16 can work through its E6 protein, which can affect the cell cycle and cause cancer. The E6 protein of HPV16 can be expressed in various cancers, including but not limited to cervical cancer, oropharyngeal cancer, anal cancer, anal canal cancer, anorectal cancer, vaginal cancer, vulvar cancer and penile cancer.

[0004] T cells can specifically recognize HPV16 E6 antigen by ...

Claims

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