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A kind of mixed-source terpenoid compound with hepatoprotective effect and use thereof

A technology of mixed-source terpenoids and compounds is applied in the field of mixed-source terpenoids and in the field of preparing liver-protecting and protecting drugs, which can solve the problems of little research on biological activity, no liver-protecting and liver-protecting effects, etc. The effect of good research and development prospects

Active Publication Date: 2022-06-21
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the biogenic pathway of 3,5-dimethylorsanic acid mixed-source terpene can be obtained from the fermentation product of P. In the three aspects of antibacterial, anti-inflammatory and anti-tumor, there are no reports about the liver-protecting effect of this kind of mixed-source terpene compounds

Method used

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  • A kind of mixed-source terpenoid compound with hepatoprotective effect and use thereof
  • A kind of mixed-source terpenoid compound with hepatoprotective effect and use thereof
  • A kind of mixed-source terpenoid compound with hepatoprotective effect and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of mixed-source terpenoids, comprises the steps:

[0033] (1) An endophytic fungus was isolated from Edgeworthia chrysantha, a traditional Chinese medicine of the genus Rupiaceae Mold Penicillium purpurogenum.

[0034] (2) Preparation of strain liquid seed liquid: the culture liquid formula is 200g of potato, 10g of yeast extract, 6g of peptone, 30g of glucose, KH 2 PO 4 2g, MgSO 4 0.5g, dilute to 1L, and adjust pH to 6.8. Dispense into 500mL conical flasks, each bottle contains about 150mL, 115℃, 68kPa, sterilize for 30 minutes. After inoculation, the cells were cultured at 28°C and 155 rpm for 3 days. Then the culture solution was diluted 100 times and used as seed solution for later use.

[0035] (3) Strain solid fermentation: 150g of rice and 180mL of purified water per 1L triangular bottle. Sterilize at 115°C, 68 kPa for 30 minutes. Add 20 mL of seed solution to each bottle and cultivate at room temperature for 40 days.

[0036] (4...

experiment example 1

[0049] Experimental Example 1: Evaluation of the protective effect of compounds on human hepatocyte injury caused by acetaminophen (APAP) in vitro

[0050] 1. Effects of compounds on the proliferation of HepG2 cells

[0051] The MTT method was used. Human hepatoma HepG2 cells (the cells better retain the characteristics of normal human hepatocytes and are often used in liver injury research) were seeded in 96-well cell culture plates, and after culturing for 24 hours, different concentrations of the samples or compounds to be tested were added. A solvent control group was set, and three parallel wells were set for each drug concentration. After the cells were treated with the drug for 48 hours, the culture medium was discarded, and 100 μL of MTT (0.5 mg / mL) solution was added to each well, and the culture was continued for 4 hours. The MTT solution was discarded, and 150 μL of DMSO was added to each well. The absorbance value was measured at the wavelength. Cell viability (...

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PUM

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Abstract

The invention discloses a mixed-source terpenoid compound with the function of protecting the liver. The mixed-source terpenoid compound has the structures shown in formula (I) and formula (II): experiments prove that the formula (I) and formula (II) of the present invention The mixed-source terpenoids shown in (II) have significant protective effects on the liver cell damage caused by paracetamol, suggesting that the mixed-source terpenoids shown in formula (I) and formula (II) can be used as liver-protecting drugs with good research and development prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine, and particularly relates to a mixed-source terpenoid compound and its use in preparing a liver-protecting drug. Background technique [0002] The liver is an important organ with metabolic functions in the body, and has the functions of storing glycogen and synthesizing secretory proteins. At the same time, it is the largest digestive gland in the human digestive system. It produces bile and secretes it into the intestine to assist digestion, and is responsible for the human body's important physiological functions. However, due to factors such as drug use, drinking, improper diet, and excessive fatigue, it is very easy to cause liver damage. Among them, liver damage caused by drugs is the most serious and is one of the most common and serious adverse drug reactions. It has risen to the No. 1 cause of death in the world. Five, so far there is still a lack of simple, objective and specific diagn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/22C07D493/16A61K31/366A61P1/16
CPCC07D493/22C07D493/16A61P1/16
Inventor 林生孙华石建功夏桂阳武玉卓张丹王玲燕张景芳李梅张凡
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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