Method for producing bacillus polymyxa additive through Baijiu distillers' grains and application of bacillus polymyxa additive

A technology for Bacillus polymyxa and liquor lees is applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., and can solve the problems that the nutritional value of fermented products is not significantly improved, the fermentation process is cumbersome, and the input cost is high. Improve the content of crops, change the micro-ecological environment, and improve the effect of immunity

Active Publication Date: 2019-11-12
HUAZHONG AGRI UNIV +1
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing fermentation microorganisms using distiller's grains of liquor mostly adopt the form of mixed bacteria, but due to the differences in the suitable conditions for the fermentation of various strains, the fermentation process is cumbersome and takes a long time, and the nutritional value of the fermentation product has not been significantly improved. The number of bacteria does not have a high number of viable bacteria due to the existence of multiple strains, resulting in relatively high input costs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing bacillus polymyxa additive through Baijiu distillers' grains and application of bacillus polymyxa additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] A method for producing bacillus polymyxa additive by utilizing distiller's grains of distiller's liquor, comprises the following steps:

[0066] (1) Put the fresh distiller's grains in the blender, set the initial temperature to 100°C, and the stirring speed to 500rpm. After keeping it for 30 minutes, reduce the stirring speed to 200rpm, and use a gradient cooling method (cooling once every 20min, followed by 5°C, 10°C, 15°C...), cooling from 100°C to 45°C within two hours, keeping warm at 45°C until the water content of the distiller's grains is 20%, and then removing the rice husks in the distiller's grains to obtain pretreated distiller's grains;

[0067] (2) Add 10% puffed corn flour in mass ratio to the obtained pretreated distiller's grains, after mixing evenly, add the Bacillus polymyxa fermentation broth with a mass ratio of 1% according to the total amount of pretreated distiller's grains, and mix to form a fermented The base material is then piled up into a sq...

Embodiment 2

[0071] A method for producing bacillus polymyxa additive by utilizing distiller's grains of distiller's liquor, comprises the following steps:

[0072] (1) (1) with embodiment 1;

[0073] (2) Add 10% puffed corn flour in mass ratio to the obtained pretreated distiller's grains, after mixing evenly, add 3% of the Bacillus polymyxa fermentation broth with a mass ratio of 3% according to the total amount of pretreated distiller's grains, and mix to form a fermented The base material is then piled up into a square structure of 15cm. It is fermented aerobically at 37°C and the humidity is controlled at 50%. When the temperature rises to 40°C, the material is turned over once, and when the temperature rises to 50°C, the material is turned over every 3 hours for co-fermentation 72h, obtain the fermentation product; wherein, keep the water content of the fermentation material at 50% during the fermentation process;

[0074] (3) is the same as (3) of embodiment 1.

[0075]Measure the...

Embodiment 3

[0077] (1) (1) with embodiment 1;

[0078] (2) Adding 15% puffed corn flour in mass ratio to the obtained pretreated distiller's grains, after mixing evenly, adding 5% of the Bacillus polymyxa fermentation broth with a mass ratio of 5% according to the total amount of pretreated distiller's grains, and mixing to form a fermented The base material is then piled up into a square structure of 20cm. It is fermented aerobically at 37°C and the humidity is controlled at 45%. When the temperature rises to 40°C, the material is turned once, and when the temperature rises to 50°C, the material is turned every 3 hours for co-fermentation 96h, obtain the fermentation product; wherein, keep the water content of the fermentation material at 50% during the fermentation process;

[0079] (3) is the same as (3) of embodiment 1.

[0080] Measure the bacterium content with PCA counting method, the bacillus mutans additive bacterium content of the preparation of above-mentioned embodiment is 2....

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for producing a bacillus polymyxa additive through Baijiu distillers' grains. The method comprises the following steps of S1, pretreating distillers' grains; S2, performing activation and enlarged culture on bacillus polymyxa so as to obtain bacillus polymyxa fermentation liquid; S3, supplementing puffed corn flour of which the mass percentage is 10% to the pretreated distillers' grains, performing uniform mixing, adding the bacillus polymyxa fermentation liquid of which the mass percentage is 1-5% of the total mass of the pretreated distillers' grains, performing mixing to obtain fermentation base materials, and then performing heap fermentation for 72-96h so as to obtain fermentation products, wherein in the fermentation process, the water content of fermentation materials is kept to be 45%; and S4, drying the obtained fermentation products, and performing crushing and screening so as to obtain the bacillus polymyxa additive. The invention also discloses an application of the additive to animal feeds and planting of crops. The additive can improve immunity and growth speed of animals, can also increase total nitrogen content and total phosphorus content in soil, and can increase crop yield and reduce diseases.

Description

technical field [0001] The invention belongs to the technical field of additive preparation, and in particular relates to a method for producing a bacillus polymyxa additive by using distiller's grains of liquor and its application. Background technique [0002] Distiller's grains are the main by-products of the brewing industry. Distiller's grains refer to the by-products of grain wine brewing, mainly including distiller's grains, beer's grains and distiller's grains. As a big country of liquor production and consumption in China, the output of distiller's grains, a by-product of liquor brewing, has also increased year after year. According to reports, in recent years, my country's liquor production has reached 1.4×10 7 t, the yield of distiller's grains also reached 5.5×10 7 t. Liquor brewing is generally fermented and distilled from sorghum, wheat, and rice. Under the action of koji microorganisms, a large amount of soluble carbohydrates in the raw materials are ferment...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75C05G3/00C12R1/12
CPCC12N1/20A23K10/18A23K50/75C05G3/00C05F5/008C05F11/00C05F11/08Y02P60/87
Inventor 彭楠刘云常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap